{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T04:22:31Z","timestamp":1777868551576,"version":"3.51.4"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The co-administration of ibuprofen (IBU) and phenyramidol HCl (PHE) is frequently preferred for the management of musculoskeletal disorders to enhance therapeutic efficacy. This study aimed to develop and validate a rapid, sensitive and cost-effective high\u2013performance liquid chromatography\u2013photodiode array (HPLC\u2013PDA) method for the simultaneous determination of IBU and PHE in human plasma and pharmaceutical preparations, and to demonstrate its applicability in pharmacokinetic studies. Chromatographic separation was achieved on a C18 column using a mobile phase consisting of phosphate buffer (pH\u00a06.0\u2009\u00b1\u20090.05) and methanol (30:70, v\/v) at a flow rate of 1.0\u00a0mL\u00b7min \u22121. The retention times of IBU and PHE were 3.7 and 2.7\u00a0min, respectively, with a total analysis time of 6\u00a0min. The method showed excellent linearity within the range of 0.05\u201340.0\u00a0\u03bcg\u00b7mL \u22121 for both drugs, with LLOQ values of 0.05\u00a0\u03bcg\u00b7mL\u22121 for IBU and 0.0025\u00a0\u03bcg\u00b7mL\u22121 for PHE. The plasma samples were collected at four time points (0.0, 1.0, 4.0 and 8.0\u00a0h) after oral administration of IBU and PHE. Cmax, Tmax and AUC0\u20138 were calculated, with Cmax of 16.14\u00a0\u03bcg\u00b7mL\u22121 for IBU and 3.71\u00a0\u03bcg\u00b7mL\u22121 for PHE, while Tmax was 1.0\u00a0h for both. Despite limitations in the number of sampling time points, the method proved highly applicable for determining pharmacokinetic profiles and for routine quality control analyses. Its short analysis time, high sensitivity and low sample volume requirement make it a powerful tool for clinical research and therapeutic monitoring. This is the first validated HPLC\u2013PDA method applied to the simultaneous pharmacokinetic evaluation of IBU and PHE in human plasma.<\/jats:p>","DOI":"10.1093\/chromsci\/bmag015","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T11:21:42Z","timestamp":1776079302000},"source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous bioanalytical method development and validation of ibuprofen and phenyramidol HCl in human plasma using high\u2013performance liquid chromatography with photodiode array detection and its application in a pharmacokinetic study"],"prefix":"10.1093","volume":"64","author":[{"given":"Nurgul","family":"K\u0131z\u0131ltas","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Ataturk University , University Road, Yakutiye, Erzurum Province 25240 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