{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T18:07:21Z","timestamp":1788890841067,"version":"build-2803163510"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study aimed to develop and validate a simple, rapid and stability-indicating high-performance liquid chromatographic (HPLC) method for the simultaneous quantification of nystatin (Nys), neomycin sulfate (NS), triamcinolone acetonide (TAC) and gramicidin D (Gr) in bulk drug samples and transferosomal gel formulations. Chromatographic separation was achieved on an Agilent Eclipse C18 (250\u2009\u00d7 4.6\u00a0mm, 5\u00a0\u03bcm) using a mobile consisting of methanol and 0.1% orthophosphoric acid (30:70, v\/v). The flow rate was maintained at 1.0\u00a0mL\/min, and detection was carried out at 260\u00a0nm using a Photo Diode Analyzer (PDA) detector. Under the optimized chromatographic conditions, all four analytes were eluted with symmetrical peaks, satisfactory resolution and acceptable theoretical plate counts within a run time of 12\u00a0min. The method exhibited excellent linearity over concentration ranges of 25\u2013150\u00a0\u03bcg\/mL for TAC, 62.5\u2013375\u00a0\u03bcg\/mL for Nys, 250\u20131,500\u00a0\u03bcg\/mL for NS and 6.25\u201337.5\u00a0\u03bcg\/mL for Gr, with correlation coefficients greater than 0.999 for all analytes. The developed method was validated according to International Council for Harmonization guidelines and demonstrated acceptable accuracy, with percentage recoveries ranging from 98 to 102% and precision, with percentage relative standard deviation values below 2%. Forced degradation studies were performed under acidic, alkaline, oxidative, reductive, thermal, photolytic, peroxide and hydrolytic conditions. The analytes exhibited degradation ranging from ~11 to 12.1%, confirming the stability-indicating capability of the method. The proposed method is simple, accurate, precise, robust and suitable for the routine quantification of Nys, NS, TAC and Gr in bulk drug substances, transferosomal gel formulations and marketed pharmaceutical products.<\/jats:p>","DOI":"10.1093\/chromsci\/bmag016","type":"journal-article","created":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T11:39:06Z","timestamp":1776598746000},"source":"Crossref","is-referenced-by-count":0,"title":["\u201cSimultaneous quantification of nystatin, triamcinolone acetonide, neomycin sulfate and gramicidin D in transferosomal gel by rapid stability indicating HPLC method with PDA detector\u201d"],"prefix":"10.1093","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9583-9834","authenticated-orcid":false,"given":"Sasikala","family":"Allam","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, Maharajah\u2019s College of Pharmacy , Phool Baugh, Vizianagaram, Andhra Pradesh 535002 ,","place":["India"]},{"name":"Department of Pharmaceutics, GITAM School of Pharmacy, GITAM (Deemed to be University) , Rushikonda, Visakhapatnam, Andhra Pradesh 530045 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6670-7071","authenticated-orcid":false,"given":"Annammadevi","family":"G S","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, GITAM School of Pharmacy, GITAM (Deemed to be University) , Rushikonda, Visakhapatnam, Andhra Pradesh 530045 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5203-7839","authenticated-orcid":false,"given":"Gowri","family":"Gollu","sequence":"additional","affiliation":[{"name":"Department of Analysis, Raghu College of Pharmacy , Dakamarri Village, Bheemunipatnam Mandal, Visakhapatnam District, Andhra Pradesh-531162 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandeepchary","family":"Padakanti","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, NIPER , Hyderabad, Telangana - 500037, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nalini","family":"Mathala","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, P P Savani (Deemed to be University) , Dhamdod Village, Mangrol, Kosamba, Surat, Gujarat - 394125 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lekhya","family":"Gurijala","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Maharajah\u2019s College of Pharmacy , Phool Baugh, Vizianagaram, Andhra Pradesh 535002 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepthi","family":"Vemuri","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics , Avanti Institute of Pharmaceutical Sciences, Cherukupally, Bhogapuram, Visakhapatnam, Andhra Praadesh-531162 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janardhan Swamy","family":"Adabala","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Saveetha College of Pharmacy, SIMATS , Saveetha Nagar, Thandalam, Chennai, Tamil Nadu - 602105 ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,8]]},"reference":[{"issue":"3","key":"2026090813184829400_ref1","doi-asserted-by":"publisher","first-page":"1532","DOI":"10.1016\/C2013-0-19020-4","article-title":"Antifungal agents. Principles and practice of pediatric infectious diseases","volume":"293","author":"Rachel","year":"2018","journal-title":"Clinical Infectious Diseases"},{"issue":"1","key":"2026090813184829400_ref2","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1111\/j.1749-6632.2002.tb04200.x","article-title":"Mechanisms of corticosteroid resistance in rheumatoid arthritis: a putative role for the corticosteroid receptor beta isoform","volume":"966","author":"Chikanza","year":"2002","journal-title":"Annals of New York Academy of Sciences"},{"issue":"1","key":"2026090813184829400_ref3","doi-asserted-by":"publisher","first-page":"116","DOI":"10.25004\/IJPSDR.2009.010214","article-title":"Formulation and evaluation of neomycin sulphate ointment containing natural wound healing agent curcuma longa","volume":"8","author":"Akanksha","year":"2009","journal-title":"International Journal of Pharmaceutical Sciences and Drug Research"},{"key":"2026090813184829400_ref4","first-page":"17","article-title":"Formulation and evaluation of neomycin sulphate ointment containing natural wound healing agent couroupita guianensis","volume":"1(1)","author":"Sandhyarani","year":"2014","journal-title":"European Journal of Pharmaceutical Sciences and Research"},{"issue":"3","key":"2026090813184829400_ref5","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1016\/j.jpba.2006.09.041","article-title":"Simultaneous determination of neomycin sulfate and polymyxin b sulfate by capillary electrophoresis with indirect UV detection","volume":"43","author":"Srisom","year":"2007","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"key":"2026090813184829400_ref6","volume-title":"From molecules to diseases","author":"Alvarez-Leefmans","year":"2009"},{"issue":"4","key":"2026090813184829400_ref7","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/s1386-1425(02)00216-0","article-title":"Ion-association method for the colorimetric determination of neomycin sulphate in pure and dosage forms","volume":"59","author":"Amin","year":"2003","journal-title":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"},{"issue":"1","key":"2026090813184829400_ref8","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1081\/SL-120013136","article-title":"Fluorimetric determination of aminoglycoside antibiotics in pharmaceutical preparations and biological fluids","volume":"35","author":"El-Shabrawy","year":"2002","journal-title":"Spectroscopy Letters"},{"issue":"1","key":"2026090813184829400_ref9","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.vibspec.2011.01.003","article-title":"A near-infrared spectroscopy method to determine aminoglycosides in pharmaceutical formulations","volume":"56","author":"Sarragu\u00eda","year":"2011","journal-title":"Vibrational Spectroscopy"},{"key":"2026090813184829400_ref10","doi-asserted-by":"publisher","first-page":"305\u2013309","DOI":"10.1007\/BF01244771","article-title":"Spectrophotometric determination of amikacin, kanamycin, neomycin and tobramycin","volume":"102","author":"Confino","year":"1990","journal-title":"Microchimica Acta"},{"issue":"6","key":"2026090813184829400_ref11","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1080\/00032719008053442","article-title":"Extraction and spectrophotometric determination of neomycin sulfate in pharmaceutical oral suspensions","volume":"23","author":"Marques","year":"1990","journal-title":"Analytical Letters"},{"issue":"4","key":"2026090813184829400_ref12","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1016\/j.jpba.2004.08.011","article-title":"Development of a liquid chromatographic method for ear drops containing neomycin sulphate, polymyxin B sulphate and dexamethasone sodium phosphate","volume":"36","author":"Pendela","year":"2004","journal-title":"Journal of Pharmaceutical Biomedical Analysis"},{"issue":"4","key":"2026090813184829400_ref13","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1016\/s0731-7085(96)01881-x","article-title":"Liquid chromatographic analysis of a formulation containing polymyxin, gramicidin and neomycin","volume":"l15","author":"Adams","year":"1997","journal-title":"Journal of Pharmaceutical Biomedical Analysis"},{"issue":"1\u20132","key":"2026090813184829400_ref14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.chroma.2003.10.111","article-title":"Ion-chromatographic determination of carbocysteine in pharmaceuticals based on non-suppressed conductimetric detection","volume":"1026","author":"Megoulas","year":"2004","journal-title":"Journal of Chromatography A"},{"issue":"4","key":"2026090813184829400_ref15","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/S0731-7085(00)00438-6","article-title":"Simultaneous identification and quantitative determination of neomycin sulfate, polymixin B sulfate, zinc bacitracin and methyl and propyl hydroxybenzoates in ophthalmic ointment by TLC","volume":"24","author":"Krzek","year":"2001","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"issue":"4","key":"2026090813184829400_ref16","doi-asserted-by":"publisher","first-page":"1068","DOI":"10.1093\/jaoac\/92.4.1068","article-title":"Determination of neomycin in the form of neomycin derivative with dabsyl chloride by thin layer chromatography and densitometry","volume":"92","author":"Hubicka","year":"2009","journal-title":"Journal of AOAC International"},{"issue":"1","key":"2026090813184829400_ref17","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.1080\/10717544.2022.2053761","article-title":"Hyaluronan-modified transfersomes based hydrogel for enhanced transdermal delivery of indomethacin","volume":"29","author":"Yuan","year":"2022","journal-title":"Drug Delivery"},{"issue":"5","key":"2026090813184829400_ref18","doi-asserted-by":"publisher","first-page":"711","DOI":"10.1080\/22297928.2019.1696227","article-title":"A stability indicating reversed phase HPLC method for estimation of trans-resveratrol in oral capsules and nanoliposomes","volume":"9","author":"Jagwani","year":"2019","journal-title":"Analytical Chemistry Letters"},{"issue":"3","key":"2026090813184829400_ref19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5530\/ijper.56.3.140","article-title":"Development and validation of UV-spectrophotometric method for estimation of berberine hydrochloride in tree turmeric, marketed formulation & PLGA nanoparticles","volume":"22","author":"Tavade","year":"2022","journal-title":"Indian Journal of Pharmaceutical Education and Research"}],"container-title":["Journal of Chromatographic Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/64\/8\/bmag016\/70997970\/bmag016.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/64\/8\/bmag016\/70997970\/bmag016.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T17:18:52Z","timestamp":1788887932000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/chromsci\/article\/doi\/10.1093\/chromsci\/bmag016\/8788169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,31]]}},"URL":"https:\/\/doi.org\/10.1093\/chromsci\/bmag016","relation":{},"ISSN":["0021-9665","1945-239X"],"issn-type":[{"value":"0021-9665","type":"print"},{"value":"1945-239X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,8,31]]},"article-number":"bmag016"}}