{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T23:54:40Z","timestamp":1781826880910,"version":"3.54.5"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The genus Tabebuia is widely used in traditional medicine of tropical and subtropical countries. We recently reported the antitumor activity toward several human cell lines of the ethyl acetate extract of the leaves of Tabebuia hypoleuca, a species endemic to Cuba, together with the high content of triterpenic acids. This work describes the development and the validation of a reversed-phase high-performance liquid chromatography with diode array detection method for the simultaneous quantitation of the contents of oleanolic acid (OA) and ursolic acid (UA). The method was carried out on a C18 column, using methanol and water as mobile phase (ratio 95:5\u00a0v\/v), at the flow rate of 0.4\u00a0mL\/min, recorded at \u03bb\u2009=\u2009210\u00a0nm. The retention times were 16.371\u2009\u00b1\u20090.045 and 16.943\u2009\u00b1\u20090.052\u00a0min for OA and UA, respectively, with a resolution between peaks of 1.29. The average contents of OA and UA within three sets of the ethyl acetate extract, considered as an active pharmaceutical ingredient (API) were 102.93 and 244.28\u00a0mg\/g, respectively. The linearity of the response was found to be 10\u2013700\u00a0\u03bcg\/mL for OA and its equation was y\u2009=\u20090.2946x \u2212 2.0432 (r2\u2009=\u20090.9924). The linearity range for UA was 5\u20131000\u00a0\u03bcg\/mL and its equation was y\u2009=\u20090.3284x \u2212 17.508 (r2\u2009=\u20090.9865). The limit of detection and limit of quantification of these constituents were 6.50 and 19.81\u00a0\u03bcg\/mL for OA, 0.75 and 1.98\u00a0\u03bcg\/mL for UA. OA and UA may be used as markers for the quality assessment of formulations containing this API. The developed method is accurate, specific, precise, reproducible and may be applied as a simple and rapid quality-control procedure for medicinal plant extracts with similar triterpene patterns.<\/jats:p>","DOI":"10.1093\/chromsci\/bmag021","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:40:52Z","timestamp":1779277252000},"source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous quantitation of oleanolic and ursolic acids by RP-HPLC-DAD on active ingredient from\n                    <i>Tabebuia hypoleuca<\/i>\n                    leaves"],"prefix":"10.1093","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2390-3299","authenticated-orcid":false,"given":"Luz Mar\u00eda S\u00e1nchez","family":"Perera","sequence":"first","affiliation":[{"name":"Biopharmaceutical Development Department, National Center of Animal Health, CENSA , Apdo 10 CP, San Jos\u00e9 de las Lajas, Mayabeque 32700 ,","place":["Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susandy Hern\u00e1ndez","family":"M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Biopharmaceutical Development Department, National Center of Animal Health, CENSA , Apdo 10 CP, San Jos\u00e9 de las Lajas, Mayabeque 32700 ,","place":["Cuba"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chouaha","family":"Bouzidi","sequence":"additional","affiliation":[{"name":"Team Natural Products, Analysis, Synthesis, UMR CNRS 8038 Cibles th\u00e9rapeutiques et conception de m\u00e9dicaments, Facult\u00e9 de Pharmacie de Paris, Universit\u00e9 Paris Cit\u00e9 , 4 Av. de l'Observatoire , 75006 Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00ebl","family":"Grougnet","sequence":"additional","affiliation":[{"name":"Team Natural Products, Analysis, Synthesis, UMR CNRS 8038 Cibles th\u00e9rapeutiques et conception de m\u00e9dicaments, Facult\u00e9 de Pharmacie de Paris, Universit\u00e9 Paris Cit\u00e9 , 4 Av. de l'Observatoire , 75006 Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"2026061819371573000_ref1","first-page":"257","article-title":"Anti-infectious activity in plants of the genus Tabebuia\/Actividad anti-infecciosa en plantas del genero Tabebuia\/Atividade antiinfecciosa em plantas do genero Tabebuia","volume":"18","author":"Jimenez-Gonzalez","year":"2013","journal-title":"Revista Universitas Scientarum"},{"key":"2026061819371573000_ref2","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/BF02805542","article-title":"A revision of Tabebuia (bignoniaceae) in Central America","volume":"22","author":"Gentry","year":"1970","journal-title":"Brittonia"},{"key":"2026061819371573000_ref3","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/978-94-024-1552-0_40","volume-title":"Medicinal and aromatic plants of South America","author":"Bussmann","year":"2018"},{"issue":"9","key":"2026061819371573000_ref4","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.3390\/molecules28093801","article-title":"Anti-inflammatory effect of ethanolic extract from Tabebuia rosea (Bertol.) 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