{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T10:21:51Z","timestamp":1771669311014,"version":"3.50.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T00:00:00Z","timestamp":1561680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Vision Group of Science and Technology, Government of Karnataka","award":["GRD-308"],"award-info":[{"award-number":["GRD-308"]}]},{"DOI":"10.13039\/501100004828","name":"Grand Challenges Canada","doi-asserted-by":"publisher","award":["R-ST-POC-1707-07275"],"award-info":[{"award-number":["R-ST-POC-1707-07275"]}],"id":[{"id":"10.13039\/501100004828","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000023","name":"Government of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000023","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methyl malonic acid and branched-chain keto acids are important biomarkers for the diagnosis of cobalamin deficiencies and maple syrup urine disease. We report the development and validation of a HILIC-ESI-MS2 method for the quantification of these organic acids from neonatal urine. The samples were 100 times diluted and analyzed on a ZIC-HILIC column with 25-mM formic acid in water: 25-mM formic acid in acetonitrile (45:55) at a flow rate of 0.8\u00a0mL\/min with a runtime of only 6\u00a0minutes. The method demonstrated a lower limit of detection of 10\u00a0ng\/mL, Limit of Quantification (LOQ) of 50\u00a0ng\/mL, linearity of r2\u00a0\u2265\u00a00.990 and recoveries of 87\u2013105% for all analytes. The intraday and interday precision CV\u2019s were &lt;10% and 12%, respectively. Extensive stability studies demonstrated the analytes to be stable in stock and in matrix with a percent change within \u00b115%. The Bland\u2013Altman analysis of the developed method with the gold standard GCMS method demonstrated a bias of 0.44, 0.11, 0.009 and \u20130.19 for methyl malonic acid, 3-methyl-2-oxovaleric acid, 2-hydroxy-3methylbutyric acid and 4-methyl-2-oxovaleric acid, respectively, proving the methods are comparable. The newly developed method involves no derivatization and has a simple sample preparation and a low runtime, enabling it to be easily automated with a high sample throughput in a cost-effective manner.<\/jats:p>","DOI":"10.1093\/chromsci\/bmz045","type":"journal-article","created":{"date-parts":[[2019,5,21]],"date-time":"2019-05-21T11:10:33Z","timestamp":1558437033000},"page":"715-723","source":"Crossref","is-referenced-by-count":9,"title":["Novel HILIC-ESI-MS method for urinary profiling of MSUD and methylmalonic aciduria biomarkers"],"prefix":"10.1093","volume":"57","author":[{"given":"Elizabeth Mary","family":"Mathew","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pragna","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Nalini","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asha","family":"Kamath","sequence":"additional","affiliation":[{"name":"Department of Statistics, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudheer","family":"Moorkoth","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,6,28]]},"reference":[{"key":"2019091012290916900_ref1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.2147\/TACG.S125962","article-title":"Maple syrup urine disease: Mechanisms and management","volume":"10","author":"Blackburn","year":"2017","journal-title":"The Application of Clinical Genetics"},{"key":"2019091012290916900_ref2","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s13023-014-0130-8","article-title":"Proposed guidelines for the diagnosis and management of methylmalonic and propionic 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