{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:04:18Z","timestamp":1787889858859,"version":"build-2784847793"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Application Basic Research Programs of Qinghai Province","award":["2015-ZI-729"],"award-info":[{"award-number":["2015-ZI-729"]}]},{"name":"Development Project of Qinghai Key Laboratory","award":["2017-ZJ-Y08"],"award-info":[{"award-number":["2017-ZJ-Y08"]}]},{"name":"CAS Key Technology Talent Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Oxytropis falcata Bunge, known as \u201cthe King of Herbs\u201d in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation and anthrax. However, it is difficult to isolate compound with high-purity from O. falcata because of its complexity. In this work, an efficient method was successfully established for the separation of 3-hydroxy-3-methylglutaryl (HMG) flavonoid glycosides from O. falcata by 2D preparative chromatography (2D-HPLC). An ODS C18 preparative column was used for the first-dimension preparation, and the XCharge C18 preparative column with different separation selectivity to the ODS C18 stationary phase was used for the second-dimension preparation. Four HMG flavonoid glycosides (oxytroflavosides A\u2013D) and two flavonoid glycosides (oxytroflavosides F and G) were separated with purities over 98%. Their structures were elucidated by nuclear magnetic resonance spectroscopy. The 2D-HPLC method used in this study is effective for preparative separation of HMG flavonoid glycosides from O. falcata. Additionally, this method shows great potential for the separation of flavonoid glycosides from other plant extracts.<\/jats:p>","DOI":"10.1093\/chromsci\/bmz091","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T07:13:32Z","timestamp":1570000412000},"page":"211-216","source":"Crossref","is-referenced-by-count":4,"title":["Separation of 3-Hydroxy-3-Methylglutaryl Flavonoid Glycosides from Oxytropis falcata Using 2D Preparative Chromatography"],"prefix":"10.1093","volume":"58","author":[{"given":"Chen","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning road, Xining 810001, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyang","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning road, Xining 810001, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilin","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Tibetan Medicine Pharmacology and Safety Evaluation, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xinning road, Xining 810001, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Chi","sequence":"first","affiliation":[{"name":"Institute of Light Industry in Qinghai Province, 2 Qingnian road, Xining 810008, P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchao","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Light Industry in Qinghai Province, 2 Qingnian road, Xining 810008, P.R. 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