{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:22:50Z","timestamp":1788348170823,"version":"build-2803163510"},"reference-count":30,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Echinococcosis Clinical Medical Research Center of Qinghai Province"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Alveolar echinococcosis (AE) is a serious parasitic disease caused by the larvae of Echinococcus multilocularis. It is the less common but substantially more deadly of the 2 major echinococcosis diseases that can occur globally but are concentrated in central Asia.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We analyzed parasite circulating cell-free DNA (cfDNA) in 149 plasma samples using a DNA sequencing\u2013based method (105 AE, 16 cystic echinococcosis, 4 liver cancer, 4 gallstones, and 20 healthy volunteers). After identifying the Echinococcus-specific cfDNA (Em-cfDNA) sequences in the samples, we determined whether Em-cfDNA could be used for AE diagnosis and as a potential indicator of the effectiveness of surgical treatment. We also examined potential associations between Em-cfDNA levels and clinical features of AE patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our work demonstrates that varying reads of Em-cfDNA were detectable in the plasma of 100% of preoperative AE patients and that all of the non-AE patients and healthy volunteers were negative. Em-cfDNA has good sensitivity and specificity for the diagnosis of AE. We also found that Em-cfDNA levels apparently have reference value for evaluating the therapeutic efficacy of surgery interventions for AE lesions. Finally, our analysis revealed that Em-cfDNA levels can reflect meaningful information about lesion size in preoperative AE patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We demonstrate that sequencing-based monitoring of Em-cfDNA can be used in the clinic as a powerful diagnostic indicator for AE. We also note that there is a strong potential for use of this liquid-biopsy method to monitor ongoing disease status in postintervention AE patients.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciaa1679","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T20:13:10Z","timestamp":1603915990000},"source":"Crossref","is-referenced-by-count":23,"title":["Parasite Circulating Cell-free DNA in the Blood of Alveolar Echinococcosis Patients as a Diagnostic and Treatment-Status Indicator"],"prefix":"10.1093","author":[{"given":"Haining","family":"Fan","sequence":"first","affiliation":[{"name":"Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gai","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China, National Engineering Research Center for Beijing Biochip Technology, Beijing, China, CapitalBio Corporation, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingqiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Ma","sequence":"additional","affiliation":[{"name":"Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijiu","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai University Affiliated Hospital, Xining, China, Qinghai Province Key Laboratory of Hydatid Disease Research, Xining, 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