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The tissue diagnosis subcommittee was tasked with determining how and when species can be determined from tissue in the absence of culture. The subcommittee reached a consensus decision that polymerase chain reaction (PCR) from tissue, but not immunohistochemistry or in situ hybridization, can be used for genus or species determination under the new EORTC\/MSGERC guidelines, but only when fungal elements are identified by histology. Fungal elements seen in tissue samples by histopathology and identified by PCR followed by sequencing should fulfill the definition of a proven fungal infection, identified to genus\/species, even in the absence of culture. This summary discusses the issues that were deliberated by the subcommittee to reach the consensus decision and outlines the criteria a laboratory should follow in order to produce data that meet the EORTC\/MSGERC definitions.<\/jats:p>","DOI":"10.1093\/cid\/ciaa1836","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:02:34Z","timestamp":1607126554000},"page":"S109-S113","source":"Crossref","is-referenced-by-count":47,"title":["Molecular Techniques for Genus and Species Determination of Fungi From Fresh and Paraffin-Embedded Formalin-Fixed Tissue in the Revised EORTC\/MSGERC Definitions of Invasive Fungal Infection"],"prefix":"10.1093","volume":"72","author":[{"given":"Shawn R","family":"Lockhart","sequence":"first","affiliation":[{"name":"Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Bialek","sequence":"additional","affiliation":[{"name":"LADR GmbH MVZ Dr. Kramer und Kollegen, Geesthacht, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher C","family":"Kibbler","sequence":"additional","affiliation":[{"name":"Centre for Medical Microbiology, University College London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Cuenca-Estrella","sequence":"additional","affiliation":[{"name":"Laboratorio de referencia e investigaci\u00f3n en Micolog\u00eda, Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik E","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios P","family":"Kontoyiannis","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Infection Control and Employee Health, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,3,12]]},"reference":[{"key":"2021031205091091600_CIT0001","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1309\/AJCP99OOOZSNISCZ","article-title":"Challenges and pitfalls of morphologic identification of fungal infections in histologic and cytologic specimens: a ten-year retrospective review at a single institution","volume":"131","author":"Sangoi","year":"2009","journal-title":"Am J Clin Pathol"},{"key":"2021031205091091600_CIT0002","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1309\/AJCPO8VTSK3HRNUT","article-title":"Diagnostic accuracy of histopathologic and cytopathologic examination of Aspergillus species","volume":"139","author":"Shah","year":"2013","journal-title":"Am J Clin Pathol"},{"key":"2021031205091091600_CIT0003","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1309\/EXBVYAUPENBM285Y","article-title":"Diagnosis of invasive septate mold infections. 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