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This is a result of considerable efforts to standardize methodology, the availability of commercial assays and external quality control programs, and additional clinical validation. This supporting article provides both clinical and technical justifications for its inclusion while also summarizing recent advances and likely future developments in the molecular diagnosis of invasive aspergillosis.<\/jats:p>","DOI":"10.1093\/cid\/ciaa1865","type":"journal-article","created":{"date-parts":[[2021,2,5]],"date-time":"2021-02-05T03:51:09Z","timestamp":1612497069000},"page":"S95-S101","source":"Crossref","is-referenced-by-count":51,"title":["<i>Aspergillus<\/i> Polymerase Chain Reaction\u2014An Update on Technical Recommendations, Clinical Applications, and Justification for Inclusion in the Second Revision of the EORTC\/MSGERC Definitions of Invasive Fungal Disease"],"prefix":"10.1093","volume":"72","author":[{"given":"P Lewis","family":"White","sequence":"first","affiliation":[{"name":"Public Health Wales Mycology Reference Laboratory, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Bretagne","sequence":"additional","affiliation":[{"name":"Mycology Laboratory, Saint Louis Hospital, Paris and Universit\u00e9 de Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M","family":"Caliendo","sequence":"additional","affiliation":[{"name":"Department of Medicine, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juergen","family":"Loeffler","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Infection, University Hospital Wuerzburg, Medical Hospital II, Wuerzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F","family":"Patterson","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Texas Health San Antonio and the South Texas Veterans Health Care System, 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