{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T06:35:09Z","timestamp":1778394909931,"version":"3.51.4"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Healthcare Group Clinician-Scientist Career Scheme","award":["CSCS\/15001"],"award-info":[{"award-number":["CSCS\/15001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum \u03b2-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.<\/jats:p>","DOI":"10.1093\/cid\/ciab848","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T11:55:28Z","timestamp":1632225328000},"page":"1850-1854","source":"Crossref","is-referenced-by-count":2,"title":["Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum \u03b2-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles"],"prefix":"10.1093","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8570-436X","authenticated-orcid":false,"given":"Sean Wei Xiang","family":"Ong","sequence":"first","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Department of Infectious Diseases, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei Yun","family":"Hon","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon Syn Hui","family":"Wee","sequence":"additional","affiliation":[{"name":"Clinical Research and Innovation Office, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan Wei Zhong","family":"Chia","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shehara","family":"Mendis","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ezlyn","family":"Izharuddin","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray Junhao","family":"Lin","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Department of Infectious Diseases, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Po Ying","family":"Chia","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Department of Infectious Diseases, Tan Tock Seng Hospital , Singapore"},{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rees Chin Swee","family":"Sim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9369-5830","authenticated-orcid":false,"given":"Mark I-Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Saw Swee Hock School of Public Health, National University of Singapore , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4063-736X","authenticated-orcid":false,"given":"Angela","family":"Chow","sequence":"additional","affiliation":[{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore"},{"name":"Saw Swee Hock School of Public Health, National University of Singapore , Singapore"},{"name":"Department of Clinical Epidemiology, Office of Clinical Epidemiology, Analytics, and Knowledge (OCEAN), Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Yoong","sequence":"additional","affiliation":[{"name":"Saw Swee Hock School of Public Health, National University of Singapore , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David Chien","family":"Lye","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Department of Infectious Diseases, Tan Tock Seng Hospital , Singapore"},{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore"},{"name":"Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine B","family":"Teng","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, National University of Singapore , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul Anantharajah","family":"Tambyah","sequence":"additional","affiliation":[{"name":"Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore , Singapore"},{"name":"Division of Infectious Disease, National University Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritu","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Division of Pediatric Infectious Diseases, Vanderbilt University , Nashville, Tennessee , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Divisions of Clinical Microbiology and Infectious Diseases, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Partha Pratim","family":"De","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Tan Tock Seng Hospital , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn","family":"Vasoo","sequence":"additional","affiliation":[{"name":"National Centre for Infectious Diseases , Singapore"},{"name":"Department of Infectious Diseases, Tan Tock Seng Hospital , Singapore"},{"name":"Lee Kong Chian School of Medicine, Nanyang Technological University , Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"2022053122505900900_CIT0001","doi-asserted-by":"crossref","first-page":"1930","DOI":"10.1007\/s00134-012-2695-9","article-title":"Characteristics and determinants of outcome of hospital-acquired bloodstream infections in intensive care units: the EUROBACT International Cohort Study","volume":"38","author":"Tabah","year":"2012","journal-title":"Intensive Care Med"},{"key":"2022053122505900900_CIT0002","doi-asserted-by":"crossref","first-page":"e202899","DOI":"10.1001\/jamanetworkopen.2020.2899","article-title":"Prevalence of antibiotic-resistant pathogens in culture-proven sepsis and outcomes associated with inadequate and broad-spectrum empiric antibiotic use","volume":"3","author":"Rhee","year":"2020","journal-title":"JAMA Netw Open"},{"key":"2022053122505900900_CIT0003","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jinf.2016.11.001","article-title":"Attributable mortality of ICU-acquired bloodstream infections: impact of the source, causative micro-organism, resistance profile and antimicrobial therapy","volume":"74","author":"Adrie","year":"2017","journal-title":"J Infect"},{"key":"2022053122505900900_CIT0004","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1186\/s12879-018-3524-8","article-title":"A systematic review of the association between delayed appropriate therapy and mortality among patients hospitalized with infections due to Klebsiella pneumoniae or Escherichia coli: how long is too long?","volume":"18","author":"Lodise","year":"2018","journal-title":"BMC Infect Dis"},{"key":"2022053122505900900_CIT0005","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1093\/cid\/civ447","article-title":"Randomized trial of rapid multiplex polymerase chain reaction-based blood culture identification and susceptibility testing","volume":"61","author":"Banerjee","year":"2015","journal-title":"Clin Infect Dis"},{"key":"2022053122505900900_CIT0006","doi-asserted-by":"crossref","first-page":"1879","DOI":"10.1007\/s10096-017-3008-6","article-title":"Impact of antimicrobial stewardship and rapid microarray testing on patients with gram-negative bacteremia","volume":"36","author":"Rivard","year":"2017","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2022053122505900900_CIT0007","first-page":"2314","article-title":"Simultaneous evaluation of diagnostic assays for pharyngeal and rectal Neisseria gonorrhoeae and Chlamydia trachomatis using a master protocol","volume":"71","author":"Doernberg","year":"2020","journal-title":"Clin Infect Dis"},{"key":"2022053122505900900_CIT0008","doi-asserted-by":"crossref","first-page":"4130","DOI":"10.1128\/JCM.01835-13","article-title":"Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles","volume":"51","author":"Altun","year":"2013","journal-title":"J Clin Microbiol"},{"key":"2022053122505900900_CIT0009"},{"key":"2022053122505900900_CIT0010"},{"key":"2022053122505900900_CIT0011","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"Poirel","year":"2011","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2022053122505900900_CIT0012","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1093\/jac\/dkp498","article-title":"Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae","volume":"65","author":"Dallenne","year":"2010","journal-title":"J Antimicrob Chemother"},{"key":"2022053122505900900_CIT0013","author":"Stevenson"},{"key":"2022053122505900900_CIT0014","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1080\/09674845.2015.11666790","article-title":"Formation of a carbapenemase resistance detection algorithm for use in the routine laboratory","volume":"72","author":"Boran","year":"2015","journal-title":"Br J Biomed Sci"},{"key":"2022053122505900900_CIT0015","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1128\/JCM.01853-16","article-title":"Comparative evaluation of four phenotypic tests for detection of carbapenemase-producing gram-negative bacteria","volume":"55","author":"No\u00ebl","year":"2017","journal-title":"J Clin Microbiol"},{"key":"2022053122505900900_CIT0016","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1128\/JCM.02068-14","article-title":"Evaluation of five chromogenic agar media and the Rosco Rapid Carb screen kit for detection and confirmation of carbapenemase production in gram-negative bacilli","volume":"53","author":"Simner","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2022053122505900900_CIT0017","doi-asserted-by":"crossref","first-page":"e0054321","DOI":"10.1128\/JCM.00543-21","article-title":"Usefulness of BioFire FilmArray BCID2 for blood culture processing in clinical practice","volume":"59","author":"Berinson","year":"2021","journal-title":"J Clin Microbiol"}],"container-title":["Clinical Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/cid\/advance-article-pdf\/doi\/10.1093\/cid\/ciab848\/40980073\/ciab848.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cid\/article-pdf\/74\/10\/1850\/43893659\/ciab848.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cid\/article-pdf\/74\/10\/1850\/43893659\/ciab848.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,31]],"date-time":"2022-05-31T22:56:11Z","timestamp":1654037771000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cid\/article\/74\/10\/1850\/6374445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,23]]},"references-count":17,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2021,9,23]]},"published-print":{"date-parts":[[2022,5,30]]}},"URL":"https:\/\/doi.org\/10.1093\/cid\/ciab848","relation":{},"ISSN":["1058-4838","1537-6591"],"issn-type":[{"value":"1058-4838","type":"print"},{"value":"1537-6591","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,5,15]]},"published":{"date-parts":[[2021,9,23]]}}}