{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:15:44Z","timestamp":1783689344118,"version":"3.55.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"10","funder":[{"name":"PROEP-IGM-Fiocruz Bahia","award":["001\/2020- ID61"],"award-info":[{"award-number":["001\/2020- ID61"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI136032"],"award-info":[{"award-number":["AI136032"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tropical Medicine Research Center"},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brasil","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Intramural Research Program"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>We have previously shown that seropositivity to rLinB-13, a salivary protein from Lutzomyia intermedia, predicted sand fly exposure and was associated with increased risk of developing cutaneous leishmaniasis (CL).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Here, we investigated the cellular immune response to saliva from Lu. intermedia, using rLinB-13 as a surrogate antigen in naturally exposed individuals presenting positive serology to LinB-13. We also investigated the response to rLinB-13 in leishmaniasis patients, displaying active ulcers and positive PCR for Leishmania braziliensis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Peripheral blood mononuclear cells (PBMCs) stimulated in vitro with rLinB-13 secreted elevated levels of interleukin-10 (IL-10), IL-4, IL-1\u03b2, IL-1\u03b1, IL-6, and chemokines (CCL3, CCL4, CCL5, and CXCL5). CL and disseminated leishmaniasis (DL) patients displayed a significantly higher immunoglobulin G (IgG) response to rLinB-13 compared with healthy subjects, and anti\u2013rLinB-13 IgG was positively correlated with the number of lesions in DL patients. Positive serology to rLinB-13 was also associated with chemotherapy failure. PBMCs from DL patients stimulated with rLINB-13 secreted significantly higher levels of IL-10 and IL-1\u03b2 compared with CL individuals.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In this study, we observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in L. braziliensis infection and results indicate that positive serology to rLinB-13 IgG can be used as a marker of DL, an emerging and severe form of disease caused by L. braziliensis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciac258","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T16:52:10Z","timestamp":1649263930000},"page":"1754-1762","source":"Crossref","is-referenced-by-count":5,"title":["Immune Response to LinB13, a <i>Lutzomyia Intermedia<\/i> Salivary Protein Correlates With Disease Severity in Tegumentary Leishmaniasis"],"prefix":"10.1093","volume":"75","author":[{"given":"Augusto M","family":"Carvalho","sequence":"first","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz , FIOCRUZ, Salvador, Bahia , Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT) , Salvador, Bahia , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayonara M","family":"Viana","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz , FIOCRUZ, Salvador, Bahia , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno B","family":"Andrade","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz , FIOCRUZ, Salvador, Bahia , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Rockville, Maryland , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesus G","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Vector Molecular Biology Section, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Rockville, Maryland , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar M","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz , FIOCRUZ, Salvador, Bahia , Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT) , Salvador, Bahia , Brazil"},{"name":"Immunology Service of the University Hospital Professor Edgard Santos, Federal University of Bahia , Salvador, Bahia , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7868-5164","authenticated-orcid":false,"given":"Camila I","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Instituto Gon\u00e7alo Moniz , FIOCRUZ, Salvador, Bahia , Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia em Doen\u00e7as Tropicais (INCT-DT) , Salvador, Bahia , 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