{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T14:30:21Z","timestamp":1789223421724,"version":"build-2803163510"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K01TW012170"],"award-info":[{"award-number":["K01TW012170"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006034","name":"University of Southern California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006034","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000888","name":"W. M. Keck Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000888","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Antimicrobial-resistant Neisseria gonorrhoeae infections are a threat to public health. Novel strategies for combating such resistance include the development of molecular assays to facilitate real-time prediction of antimicrobial susceptibility. Resistance to ciprofloxacin is determined by the presence of a single mutation at codon 91 of the gyrase A gene; molecular assays to guide therapy are commercially available. Resistance to cefixime is conferred via 1 of 6 critical mutations in either the mosaic penA gene or specific loci in the nonmosaic region. Resistance to ceftriaxone is conferred through mutations in 1 of 4 genes: penA, ponA, penB, and mtr; however, the ability to predict reduced susceptibility based on those genes varies by geographic region. Here, we highlight the work done toward the development of 3 such assays for ciprofloxacin, cefixime, and ceftriaxone, discuss the status of our current understanding and ongoing challenges, and suggest future directions.<\/jats:p>","DOI":"10.1093\/cid\/ciac371","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T05:05:57Z","timestamp":1657602357000},"page":"1655-1660","source":"Crossref","is-referenced-by-count":33,"title":["Resistance-Guided Therapy for <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4077-3476","authenticated-orcid":false,"given":"Lao-Tzu","family":"Allan-Blitz","sequence":"first","affiliation":[{"name":"Division of Global Health Equity, Department of Medicine, Brigham and Women\u2019s Hospital , Boston, Massachusetts , USA"},{"name":"Department of Pediatrics, Boston Children\u2019s Hospital , Boston, Massachusetts , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul C","family":"Adamson","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, University of California , Los Angeles, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D","family":"Klausner","sequence":"additional","affiliation":[{"name":"Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California , Los Angeles, California , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,7,12]]},"reference":[{"key":"2023053018550193800_ciac371-B1","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"Centers for Disease Control and Prevention","year":"2013"},{"key":"2023053018550193800_ciac371-B2","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1001\/jama.2015.10347","article-title":"Trends in Neisseria gonorrhoeae susceptibility to cephalosporins in the United States, 2006\u20132014","volume":"314","author":"Kirkcaldy","year":"2015","journal-title":"JAMA"},{"key":"2023053018550193800_ciac371-B3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15585\/mmwr.rr7004a1","article-title":"Sexually transmitted infections treatment guidelines, 2021","volume":"70","author":"Workowski","year":"2021","journal-title":"MMWR Recomm Rep"},{"key":"2023053018550193800_ciac371-B4","doi-asserted-by":"crossref","first-page":"e0229421","DOI":"10.1128\/aac.02294-21","article-title":"Markedly increasing antibiotic resistance and dual treatment of Neisseria gonorrhoeae isolates in Guangdong, China, from 2013 to 2020","volume":"66","author":"Lin","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"key":"2023053018550193800_ciac371-B5","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1128\/CMR.00010-14","article-title":"Antimicrobial resistance in Neisseria gonorrhoeae in the 21st century: past, evolution, and future","volume":"27","author":"Unemo","year":"2014","journal-title":"Clin Microbiol Rev"},{"key":"2023053018550193800_ciac371-B6","author":"World Health Organization"},{"key":"2023053018550193800_ciac371-B7","doi-asserted-by":"crossref","first-page":"4598","DOI":"10.1128\/JCM.00004-10","article-title":"Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus","volume":"48","author":"Malhotra-Kumar","year":"2010","journal-title":"J Clin Microbiol"},{"key":"2023053018550193800_ciac371-B8","doi-asserted-by":"crossref","first-page":"794","DOI":"10.3389\/fmicb.2019.00794","article-title":"Molecular diagnosis of drug-resistant tuberculosis; a literature review","volume":"10","author":"Nguyen","year":"2019","journal-title":"Front Microbiol"},{"key":"2023053018550193800_ciac371-B9","doi-asserted-by":"crossref","first-page":"373","DOI":"10.2165\/00003495-198835040-00003","article-title":"Ciprofloxacin. 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