{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T16:23:54Z","timestamp":1778689434672,"version":"3.51.4"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Doxycycline is used for treatment of Mansonella perstans infection. Immune modulatory effects of both M. perstans and doxycycline have been described but long-term implications on host immune response are not defined. Here we determined multiple immune parameters of M. perstans\u2013infected individuals before and after doxycycline treatment to characterize doxycycline effects on host T-cell immunity.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Immune characterization of doxycycline-treated M. perstans\u2013infected individuals was performed as part of an open-label randomized clinical trial. Immune cell population phenotyping by flow cytometry and functional in vitro T-cell assays were performed at baseline, 6 months, and \u201clong term\u201d (18\u201324 months) after treatment start. Treatment efficacy, based on peripheral blood microfilaria (mf) burden, was correlated with immune parameters and effects on immune response against concomitant Mycobacterium tuberculosis infection were determined.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Immune population phenotyping indicated changes in functional T-cell responses after doxycycline treatment. Constitutive and superantigen-induced T-cell activation and polarization towards T-helper type (TH) 1 phenotype at baseline declined after doxycycline treatment, whereas low proportions of TH17 and TH1* cells at baseline increased significantly at follow-up. In accordance, long-term decline in antigen-specific TH1 responses against concomitant M. tuberculosis infection was seen. Notably, only TH17 and TH1* changes after 6 months and TH17 at baseline were negatively correlated with M. perstans microfilaria burden or reduction, whereas long-term changes were not associated with treatment efficacy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We found long-term immune modulatory effects of doxycycline treatment leading to decreased constitutive T-cell activation, polarization towards TH17\/TH1*, and impaired immune response against concomitant M. tuberculosis infection.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciac428","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T11:14:24Z","timestamp":1654254864000},"page":"e1399-e1407","source":"Crossref","is-referenced-by-count":3,"title":["Doxycycline Treatment of <i>Mansonella perstans<\/i>\u2013Infected Individuals Affects Immune Cell Activation and Causes Long-term T-cell Polarization"],"prefix":"10.1093","volume":"76","author":[{"given":"Wilfred","family":"Aniagyei","sequence":"first","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan Kofi","family":"Adjei","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernest","family":"Adankwah","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"},{"name":"Department of Medical Diagnostics, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST) , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Seyfarth","sequence":"additional","affiliation":[{"name":"Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital D\u00fcsseldorf, Heinrich-Heine-University , D\u00fcsseldorf , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ertan","family":"Mayatepek","sequence":"additional","affiliation":[{"name":"Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital D\u00fcsseldorf, Heinrich-Heine-University , D\u00fcsseldorf , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Antwi Berko","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Asamoah","family":"Sakyi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, School of Medicine and Dentistry, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST) , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Batsa Debrah","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander Yaw","family":"Debrah","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"},{"name":"Faculty of Allied Health Sciences of Kwame Nkrumah University of Science and Technology , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achim","family":"Hoerauf","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital of Bonn , Bonn , Germany"},{"name":"German Center for Infection Research (DZIF) , Partner Site Bonn-Cologne, Bonn-Cologne , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorcas O","family":"Owusu","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard O","family":"Phillips","sequence":"additional","affiliation":[{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine , Kumasi , Ghana"},{"name":"Department of Medical Diagnostics, College of Health Sciences, Kwame Nkrumah University of Science and Technology (KNUST) , Kumasi , Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, University Hospital D\u00fcsseldorf, Heinrich-Heine-University , D\u00fcsseldorf , 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