{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:10:17Z","timestamp":1793315417607,"version":"4.5.0"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AR056647"],"award-info":[{"award-number":["R01AR056647"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Next-generation sequencing (NGS) is increasingly used for periprosthetic joint infection (PJI) diagnosis, but its clinical utility is poorly defined. Shotgun metagenomic sequencing (sNGS) has been reported to identify PJI pathogens undetected by culture in sonicate fluid. However, sNGS is complex and costly. Here, 16S ribosomal RNA (rRNA) gene-based targeted metagenomic sequencing (tNGS) was compared to sNGS of sonicate fluid for microbial detection and identification in patients with total hip arthroplasty (THA) and total knee arthroplasty (TKA) failure.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A convenience sample of sonicate fluids derived from patients who had undergone THA or TKA removal, enriched with culture negative PJI cases, was tested. Samples had been previously tested by sNGS. For tNGS, samples were extracted, amplified by polymerase chain reaction targeting the V1 to V3 regions of the 16S rRNA gene, and sequenced on an Illumina MiSeq.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>A total of 395 sonicate fluids, including 208 from subjects with PJI, were studied. Compared with sonicate fluid culture, tNGS had higher positive percent agreement (72.1 vs 52.9%, P &amp;lt; .001), detecting potential pathogens in 48.0% of culture-negative PJIs. There was no difference between the positive percent agreement of tNGS (72.1%) and sNGS (73.1%, P = .83).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>16S rRNA gene-based tNGS is a potential diagnostic tool for PJI pathogen identification in sonicate fluid from failed THAs and TKAs in culture-negative cases, with similar performance characteristics to sNGS.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciac646","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T18:38:20Z","timestamp":1660070300000},"page":"e1456-e1462","source":"Crossref","is-referenced-by-count":75,"title":["Targeted Versus Shotgun Metagenomic Sequencing-based Detection of Microorganisms in Sonicate Fluid for Periprosthetic Joint Infection Diagnosis"],"prefix":"10.1093","volume":"76","author":[{"given":"Hyo-Lim","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota , USA"},{"name":"Department of Internal Medicine, Daegu Catholic University School of Medicine , Daegu , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Flurin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota , USA"},{"name":"Department of Intensive Care, University Hospital of Guadeloupe , Pointe-\u00e0-Pitre , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J","family":"Thoendel","sequence":"additional","affiliation":[{"name":"Division of Public Health, Infectious Diseases, and Occupational Medicine, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P","family":"Abdel","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerryl E","family":"Greenwood-Quaintance","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-4141","authenticated-orcid":false,"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Division of Clinical Microbiology, Mayo Clinic , Rochester, Minnesota , USA"},{"name":"Division of Public Health, Infectious Diseases, and Occupational Medicine, Mayo Clinic , Rochester, Minnesota , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"2023053119003005000_ciac646-B1","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1016\/j.arth.2020.12.005","article-title":"Projected economic burden of periprosthetic joint infection of the hip and knee in the United States","volume":"36","author":"Premkumar","year":"2021","journal-title":"J Arthroplasty"},{"key":"2023053119003005000_ciac646-B2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1128\/CMR.00111-13","article-title":"Prosthetic joint infection","volume":"27","author":"Tande","year":"2014","journal-title":"Clin Microbiol 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