{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T04:24:08Z","timestamp":1777004648882,"version":"3.51.4"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T00:00:00Z","timestamp":1691020800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Syphilis is a complex, multistage, sexually transmitted infection (STI) caused by the bacterium Treponema pallidum subspecies pallidum (TP). New diagnostic tools are needed to minimize transmission. In this study, we aimed to assess the additional value of an investigational transcription-mediated amplification test for TP (TP-TMA) for routine diagnostics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Between September 2021 and August 2022, visits by all participants of the national preexposure prophylaxis (PrEP) program at the sexual health center (SHC) in Amsterdam were included. Anal, pharyngeal, vaginal, and urine samples collected for Chlamydia trachomatis and Neisseria gonorrhoeae screening were additionally tested with the TP-TMA assay based on detection of 23S rRNA of TP.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In total, 9974 SHC visits by 3283 participants were included. There were 191 infectious syphilis cases diagnosed: 26 (14%) primary syphilis, 54 (29%) secondary syphilis, and 111 (58%) early latent syphilis. In 79 of the 191 (41%) syphilis cases, at least 1 sample was TP-TMA\u2013positive. For 16 participants, the positive TP-TMA result was not concordant with routine diagnostics. Of those, 2 participants were treated for syphilis within a week before the visit. Eight participants were treated for a syphilis notification at the visit or for another STI. Five participants were diagnosed with syphilis at the following visit, and 1 participant was lost to follow-up.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>By adding the TP-TMA assay to routine diagnostics, we identified 14 of 191 (7%) additional syphilis infections among participants of the national PrEP program. The TP-TMA assay is a useful diagnostic tool to increase syphilis case finding and thus limit the transmission of syphilis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciad455","type":"journal-article","created":{"date-parts":[[2023,8,3]],"date-time":"2023-08-03T22:09:06Z","timestamp":1691100546000},"page":"1717-1722","source":"Crossref","is-referenced-by-count":7,"title":["Timely Diagnosis of Incubating Syphilis Infections Using <i>Treponema pallidum<\/i> Transcription-Mediated Amplification Assay"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9230-7488","authenticated-orcid":false,"given":"Helene C A","family":"Zondag","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Public Health Laboratory, Public Health Service of Amsterdam , Amsterdam , The Netherlands"},{"name":"Department of Medical Microbiology, University of Amsterdam, Amsterdam Institute for Infection & Immunity, Amsterdam Medical Center , Amsterdam , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alje P","family":"van Dam","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Public Health Laboratory, Public Health Service of Amsterdam , Amsterdam , The Netherlands"},{"name":"Department of Medical Microbiology, University of Amsterdam, Amsterdam Institute for Infection & Immunity, Amsterdam Medical Center , Amsterdam , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Bosch","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Public Health Laboratory, Public Health Service of Amsterdam , Amsterdam , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Damon","family":"Getman","sequence":"additional","affiliation":[{"name":"Hologic Inc , San Diego, California , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley","family":"Nenninger","sequence":"additional","affiliation":[{"name":"Hologic Inc , San Diego, California , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry J C","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Amsterdam, Amsterdam Institute for Infection & Immunity, Amsterdam Medical Center , Amsterdam , The Netherlands"},{"name":"Department of Infectious Diseases, Center for Sexual Health, Public Health Service Amsterdam , Amsterdam , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia M","family":"Bruisten","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Public Health Laboratory, Public Health Service of Amsterdam , Amsterdam , The Netherlands"},{"name":"Department of Medical Microbiology, University of Amsterdam, Amsterdam Institute for Infection & Immunity, Amsterdam Medical Center , Amsterdam , The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,8,3]]},"reference":[{"key":"2023121605173173600_ciad455-B1","author":"Rijksinstituut voor Volksgezondheid en Milieu-Centrum Infectieziektebestrijding-Centrum Epidemiologie en Surveillance van Infectieziekten (RIVM-CIb-EPI)","year":"2022"},{"key":"2023121605173173600_ciad455-B2","doi-asserted-by":"crossref","first-page":"17073","DOI":"10.1038\/nrdp.2017.73","article-title":"Syphilis","volume":"3","author":"Peeling","year":"2017","journal-title":"Nat Rev Dis Primers"},{"key":"2023121605173173600_ciad455-B3","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1128\/JCM.00720-09","article-title":"Clinical value of Treponema pallidum real-time PCR for diagnosis of syphilis","volume":"48","author":"Heymans","year":"2010","journal-title":"J Clin 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