{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:28:03Z","timestamp":1792481283827,"version":"4.5.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":2,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>New insights into community-acquired pleural infection microbiology may enable development of more rapid and accessible diagnostics that can streamline treatment. This study systematically compared molecular approaches for pathogen detection in pleural fluid from patients with such infections. Considering the need for rapid results with minimum hands-on time and test complexity, off-label use of the cassette-based, random-access BIOFIRE Joint Infection Panel (BF-JIP, bioM\u00e9rieux) with and without supplementary in-house polymerase chain reaction (PCR) assays was evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pleural fluids from 162 subjects from the Mayo Clinic (Rochester, Minnesota) and Haukeland University Hospital (Bergen, Norway), where molecular tests are routinely used for analyzing pleural fluid, were studied. The performance of the BF-JIP was compared to 16S rRNA gene PCR followed by Sanger and\/or next-generation sequencing (16S rRNA gene PCR\/sequencing), an in-house PCR panel specifically designed for pleural space infections, and culture.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The BF-JIP supplemented with 3 in-house PCR assays targeting Fusobacterium nucleatum group, Streptococcus intermedius\/constellatus, and Aggregatibacter aphrophilus detected 96.8% of pleural infections, providing complete microbial profiles in 70.1%. In comparison, the detection rate of 16S rRNA gene PCR\/sequencing was 92.2%, providing complete microbial profiles in 87.0%. Culture detected bacteria in only 31.9% of pleural fluids, providing complete microbial profiles in 15.3%.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results underscore the importance of molecular approaches in diagnosing pleural space infections and demonstrate the feasibility of panel-based PCR-based diagnostics for these sometimes-complex infections. The BF-JIP, designed for testing synovial fluid, could be modified to provide a useful diagnostic for testing pleural fluid.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cid\/ciag050","type":"journal-article","created":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:48:21Z","timestamp":1769690901000},"page":"e1345-e1352","source":"Crossref","is-referenced-by-count":3,"title":["Microbial Detection in Community-Acquired Pleural Space Infections Using the BIOFIRE Joint Infection Panel and Individual Polymerase Chain Reaction Assays"],"prefix":"10.1093","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4093-2809","authenticated-orcid":false,"given":"Portia","family":"Mira","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2486-5426","authenticated-orcid":false,"given":"Ruben","family":"Dyrhovden","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Haukeland University Hospital , Bergen ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marit Gjerde","family":"Tellevik","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Haukeland University Hospital , Bergen ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8725-9960","authenticated-orcid":false,"given":"Tomas Mikal","family":"Eagan","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, University of Bergen , Bergen ,","place":["Norway"]},{"name":"Department of Thoracic Medicine, Haukeland University Hospital , Bergen ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abigail","family":"Krause","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Lopez-Martinez","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Wolf","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4551-6559","authenticated-orcid":false,"given":"Kaiser G","family":"Lim","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6311-2881","authenticated-orcid":false,"given":"Ryan M","family":"Kern","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva Carmona","family":"Porquera","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4390-0370","authenticated-orcid":false,"given":"Oyvind","family":"Kommedal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Haukeland University Hospital , Bergen ,","place":["Norway"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6344-4141","authenticated-orcid":false,"given":"Robin","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]},{"name":"Department of Medicine, Mayo Clinic , Rochester, Minnesota ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,31]]},"reference":[{"key":"2026070722331034000_ciag050-B1","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1136\/thoraxjnl-2020-215267","article-title":"Epidemiology and prognostic factors of pleural empyema","volume":"76","author":"Bobbio","year":"2021","journal-title":"Thorax"},{"key":"2026070722331034000_ciag050-B2","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.cmi.2018.11.030","article-title":"The bacterial aetiology of pleural empyema. 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