{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:00:35Z","timestamp":1790402435442,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["AMR\/160\/2018-ECD-II"],"award-info":[{"award-number":["AMR\/160\/2018-ECD-II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,7,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The objective of the study was to elucidate antifungal resistance and associated azole resistance mechanisms in Candida (Candidozyma) auris isolates across India over a period of 13 years (2009\u20132021).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 596 C. auris isolates from 37 healthcare centers were collected. Antifungal susceptibility was performed and ERG11 gene was sequenced for 45 representative isolates. The ERG11 and efflux pump (MDR1 and CDR1) expression under fluconazole drug pressure was assessed by quantitative reverse-transcription polymerase chain reaction (n = 20) along with cell wall ergosterol content estimation. Short tandem repeat (STR) genotyping was done on 235 isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The highest number of isolates (50.1%) was recovered from northern India, followed by eastern (27.6%) and southern India (13.6%). The majority of the isolates were recovered from private sector hospitals. A total of 80.0% and 27.9% of isolates were resistant to fluconazole and voriconazole, respectively. A notable increase in resistance to amphotericin B and caspofungin was observed, with 3.2% of isolates being multidrug resistant. In ERG11, Y132F and K143R mutations were present in both fluconazole-susceptible and -resistant isolates; in addition, 50% of fluconazole-susceptible isolates showed elevated ERG11 expression and ergosterol content upon fluconazole treatment. Basal level cell wall ergosterol was higher in fluconazole-susceptible isolates. All isolates belonged to clade I with STR genotyping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study demonstrates high levels of antifungal resistance in the largest collection of Indian C. auris clinical isolates reported to date. Besides mutations and gene expressions, other pathways conferring drug resistance, such as stress response, must be studied to further understand the evolution of this pathogen.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cid\/ciag257","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T11:41:20Z","timestamp":1777030880000},"page":"89-99","source":"Crossref","is-referenced-by-count":2,"title":["Trends in Antifungal Resistance and Mechanistic Insights Into Azole Resistance in\n                    <i>Candida (Candidozyma) auris<\/i>\n                    : A 13-Year Study of a Comprehensive Set of Clinical Isolates From India"],"prefix":"10.1093","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9286-4193","authenticated-orcid":false,"given":"Sourav","family":"Das","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shreya","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-510X","authenticated-orcid":false,"given":"Yamini","family":"Tawde","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research , Chandigarh ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6734-361X","authenticated-orcid":false,"given":"Bram","family":"Spruijtenburg","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Radboud University Medical Center\/CWZ Hospital, Nijmegen ,","place":["The 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of Expertise for Mycology , Radboud University Medical Center\/CWZ Hospital, Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology, Radboudumc , Nijmegen ,","place":["The Netherlands"]},{"name":"Institute of Translational Research, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases and Excellence Center for Medical Mycology, University of Cologne , Cologne ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theun","family":"de Groot","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Radboud University Medical Center\/CWZ Hospital, Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital\/Dicoon , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eelco F J","family":"Meijer","sequence":"additional","affiliation":[{"name":"Radboudumc-CWZ Center of Expertise for Mycology , Radboud University Medical Center\/CWZ Hospital, Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology and Immunology, Canisius-Wilhelmina Hospital\/Dicoon , Nijmegen ,","place":["The Netherlands"]},{"name":"Department of Medical Microbiology, Radboudumc , Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamini","family":"Walia","sequence":"additional","affiliation":[{"name":"Division of Epidemiology and Communicable Diseases, Indian Council of Medical Research , New Delhi ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4871-8113","authenticated-orcid":false,"given":"Harsimran","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Postgraduate Institute of Medical 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