{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:02:09Z","timestamp":1790186529336,"version":"4.1.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T00:00:00Z","timestamp":1553040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005073","name":"Agency for Defense Development","doi-asserted-by":"publisher","award":["UD160022ID"],"award-info":[{"award-number":["UD160022ID"]}],"id":[{"id":"10.13039\/501100005073","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2017M3A9E4061992"],"award-info":[{"award-number":["NRF-2017M3A9E4061992"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014035","name":"Armed Forces Health Surveillance Branch","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014035","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Global Emerging Infections Surveillance and Response System","award":["P0025-15-ME"],"award-info":[{"award-number":["P0025-15-ME"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Endemic outbreaks of hantaviruses pose a critical public health threat worldwide. Hantaan orthohantavirus (HTNV) causes hemorrhagic fever with renal syndrome (HFRS) in humans. Using comparative genomic analyses of partial and nearly complete sequences of HTNV from humans and rodents, we were able to localize, with limitations, the putative infection locations for HFRS patients. Partial sequences might not reflect precise phylogenetic positions over the whole-genome sequences; finer granularity of rodent sampling reflects more precisely the circulation of strains.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Five HFRS specimens were collected. Epidemiological surveys were conducted with the patients during hospitalization. We conducted active surveillance at suspected HFRS outbreak areas. We performed multiplex polymerase chain reaction\u2013based next-generation sequencing to obtain the genomic sequence of HTNV from patients and rodents. The phylogeny of human- and rodent-derived HTNV was generated using the maximum likelihood method. For phylogeographic analyses, the tracing of HTNV genomes from HFRS patients was defined on the bases of epidemiological interviews, phylogenetic patterns of the viruses, and geographic locations of HTNV-positive rodents.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The phylogeographic analyses demonstrated genetic clusters of HTNV strains from clinical specimens, with HTNV circulating in rodents at suspected sites of patient infections.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study demonstrates a major shift in molecular epidemiological surveillance of HTNV. Active targeted surveillance was performed at sites of suspected infections, allowing the high-resolution phylogeographic analysis to reveal the site of emergence of HTNV. We posit that this novel approach will make it possible to identify infectious sources, perform disease risk assessment, and implement preparedness against vector-borne viruses.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cid\/ciz234","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T17:13:04Z","timestamp":1553015584000},"page":"464-473","source":"Crossref","is-referenced-by-count":32,"title":["Active Targeted Surveillance to Identify Sites of Emergence of Hantavirus"],"prefix":"10.1093","volume":"70","author":[{"given":"Won-Keun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Sun","family":"No","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, 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Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung-Ho","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujin","family":"Kim","sequence":"first","affiliation":[{"name":"Armed Forces Medical Center, Seongnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunhye","family":"Park","sequence":"first","affiliation":[{"name":"4th Research and Development Institute, Agency for Defense Development, Daejeon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungchan","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geum-Young","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyun","family":"Song","sequence":"first","affiliation":[{"name":"4th Research and Development Institute, Agency for Defense Development, Daejeon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se Hun","family":"Gu","sequence":"first","affiliation":[{"name":"4th Research and Development Institute, Agency for Defense Development, Daejeon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kkothanahreum","family":"Park","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heung-Chul","family":"Kim","sequence":"first","affiliation":[{"name":"65th Medical Brigade\/MEDDAC-Korea, Unit, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R","family":"Wiley","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick S G","family":"Chain","sequence":"first","affiliation":[{"name":"Bioscience Division, Los Alamos National Laboratory, New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Tae","family":"Jeong","sequence":"first","affiliation":[{"name":"4th Research and Development Institute, Agency for Defense Development, Daejeon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry A","family":"Klein","sequence":"first","affiliation":[{"name":"65th Medical Brigade\/MEDDAC-Korea, Unit, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Palacios","sequence":"first","affiliation":[{"name":"Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Won","family":"Song","sequence":"first","affiliation":[{"name":"Department of Microbiology, College of Medicine, Korea University, Seoul"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,20]]},"reference":[{"key":"2020031921575729800_CIT0001","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jcv.2014.08.033","article-title":"Hantaviruses\u2013globally emerging pathogens","volume":"64","author":"Kruger","year":"2015","journal-title":"J Clin Virol"},{"key":"2020031921575729800_CIT0002","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1128\/CMR.00062-09","article-title":"A global perspective on hantavirus ecology, epidemiology, and disease","volume":"23","author":"Jonsson","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"2020031921575729800_CIT0003","unstructured":"Korea Centers for Disease Control and Prevention. 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