{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T15:42:00Z","timestamp":1781624520709,"version":"3.54.5"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"F. Amant, Research Fund Flanders","award":["G080217N"],"award-info":[{"award-number":["G080217N"]}]},{"name":"Vermeesch, Research Fund Flanders","award":["G080217N"],"award-info":[{"award-number":["G080217N"]}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["C14\/18\/092"],"award-info":[{"award-number":["C14\/18\/092"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005026","name":"Stichting tegen Kanker","doi-asserted-by":"publisher","award":["FAF-C\/2018\/1209"],"award-info":[{"award-number":["FAF-C\/2018\/1209"]}],"id":[{"id":"10.13039\/501100005026","id-type":"DOI","asserted-by":"publisher"}]},{"name":"D. Villela, FAPESP scholarship","award":["2017\/23448-8"],"award-info":[{"award-number":["2017\/23448-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Numerous publications have reported the incidental detection of occult malignancies upon routine noninvasive prenatal testing (NIPT). However, these studies were not designed to evaluate the NIPT performance for cancer detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We investigated the sensitivity of a genome-wide NIPT pipeline, called GIPSeq, for detecting cancer-specific copy number alterations (CNAs) in plasma tumor DNA (ctDNA) of patients with breast cancer. To assess whether a pregnancy itself, with fetal cell-free DNA (cfDNA) in the maternal circulation, might influence the detection of ctDNA, results were compared in pregnant (n\u2009=\u200925) and nonpregnant (n\u2009=\u200925) cancer patients. Furthermore, the ability of GIPSeq to monitor treatment response was assessed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Overall GIPSeq sensitivity for detecting cancer-specific CNAs in plasma cfDNA was 26%. Fifteen percent of detected cases were asymptomatic at the time of blood sampling. GIPSeq sensitivity mainly depended on the tumor stage. Also, triple negative breast cancers (TNBC) were more frequently identified compared to hormone-positive or HER2-enriched tumors. This might be due to the presence of high-level gains and losses of cfDNA or high ctDNA loads in plasma of TNBC. Although higher GIPSeq sensitivity was noted in pregnant (36%) than in nonpregnant women (16%), the limited sample size prohibits a definite conclusion. Finally, GIPSeq profiling of cfDNA during therapy allowed monitoring of early treatment response.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>The results underscore the potential of NIPT-based tests, analyzing CNAs in plasma cfDNA in a genome-wide and unbiased fashion for breast cancer detection, cancer subtyping and treatment monitoring in a pregnant and nonpregnant target population.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/clinchem\/hvaa196","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T11:23:34Z","timestamp":1597749814000},"page":"1414-1423","source":"Crossref","is-referenced-by-count":19,"title":["Breast Cancer Detection and Treatment Monitoring Using a Noninvasive Prenatal Testing Platform: Utility in Pregnant and Nonpregnant Populations"],"prefix":"10.1093","volume":"66","author":[{"given":"Liesbeth","family":"Lenaerts","sequence":"first","affiliation":[{"name":"Department of Oncology, Laboratory of Gynecological Oncology, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiwen","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Brison","sequence":"additional","affiliation":[{"name":"Centre of Human Genetics, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Neofytou","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, Belgium"},{"name":"Cancer Research UK Cambridge Institute, Molecular and Computational Diagnostics, University of Cambridge, Cambridge, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Jatsenko","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Lefr\u00e8re","sequence":"additional","affiliation":[{"name":"Department of Oncology, Laboratory of Gynecological Oncology, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Maggen","sequence":"additional","affiliation":[{"name":"Department of Oncology, Laboratory of Gynecological Oncology, KU Leuven, Leuven, Belgium"},{"name":"Department of Gynecology and Obstetrics, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darine","family":"Villela","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, Belgium"},{"name":"Departamento de Gen\u00e9tica e Biologia Evolutiva, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Verheecke","sequence":"additional","affiliation":[{"name":"Gynaecology and Obstetrics Department, General Hospital Turnhout, Turnhout, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Dehaspe","sequence":"additional","affiliation":[{"name":"Genomics Core Facility, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca","family":"Croitor","sequence":"additional","affiliation":[{"name":"Department of Imaging and Pathology, Unit of Biomedical MRI, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigrid","family":"Hatse","sequence":"additional","affiliation":[{"name":"Department of Oncology, Laboratory of Experimental Oncology, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Wildiers","sequence":"additional","affiliation":[{"name":"Department of Oncology, Laboratory of Experimental Oncology, KU Leuven, Leuven, Belgium"},{"name":"Department of General Medical Oncology, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Neven","sequence":"additional","affiliation":[{"name":"Department of Oncology, Laboratory of Gynecological Oncology, KU Leuven, Leuven, Belgium"},{"name":"Department of Gynecology and Obstetrics, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Vandecaveye","sequence":"additional","affiliation":[{"name":"Department of Imaging and Pathology, Unit of Translational MRI, KU Leuven, Leuven, Belgium"},{"name":"Department of Radiology, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Floris","sequence":"additional","affiliation":[{"name":"Department of Imaging and Pathology, Unit of Translational Cell & Tissue Research, KU Leuven, Leuven, Belgium"},{"name":"Department of Pathology, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris Robert","family":"Vermeesch","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, Belgium"},{"name":"Centre of Human Genetics, University Hospitals Leuven, Leuven, 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