{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:09:00Z","timestamp":1793146140611,"version":"4.5.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T00:00:00Z","timestamp":1626307200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"National Cancer Institute of the National Institutes of Health","award":["R00CA190783"],"award-info":[{"award-number":["R00CA190783"]}]},{"name":"National Cancer Institute of the National Institutes of Health","award":["1R33CA229068-01A1"],"award-info":[{"award-number":["1R33CA229068-01A1"]}]},{"DOI":"10.13039\/100000936","name":"Gordon and Betty Moore Foundation","doi-asserted-by":"publisher","award":["GBMF8049"],"award-info":[{"award-number":["GBMF8049"]}],"id":[{"id":"10.13039\/100000936","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["F-1895"],"award-info":[{"award-number":["F-1895"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intraoperative tissue analysis and identification are critical to guide surgical procedures and improve patient outcomes. Here, we describe the clinical translation and evaluation of the MasSpec Pen technology for molecular analysis of in vivo and freshly excised tissues in the operating room (OR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An Orbitrap mass spectrometer equipped with a MasSpec Pen interface was installed in an OR. A \u201cdual-path\u201d MasSpec Pen interface was designed and programmed for the clinical studies with 2 parallel systems that facilitated the operation of the MasSpec Pen. The MasSpec Pen devices were autoclaved before each surgical procedure and were used by surgeons and surgical staff during 100 surgeries over a 12-month period.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Detection of mass spectral profiles from 715 in vivo and ex vivo analyses performed on thyroid, parathyroid, lymph node, breast, pancreatic, and bile duct tissues during parathyroidectomies, thyroidectomies, breast, and pancreatic neoplasia surgeries was achieved. The MasSpec Pen enabled gentle extraction and sensitive detection of various molecular species including small metabolites and lipids using a droplet of sterile water without causing apparent tissue damage. Notably, effective molecular analysis was achieved while no limitations to sequential histologic tissue analysis were identified and no device-related complications were reported for any of the patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study shows that the MasSpec Pen system can be successfully incorporated into the OR, allowing direct detection of rich molecular profiles from tissues with a seconds-long turnaround time that could be used to inform surgical and clinical decisions without disrupting tissue analysis workflows.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/clinchem\/hvab098","type":"journal-article","created":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T23:14:43Z","timestamp":1621293283000},"page":"1271-1280","source":"Crossref","is-referenced-by-count":37,"title":["Clinical Translation and Evaluation of a Handheld and Biocompatible Mass Spectrometry Probe for Surgical Use"],"prefix":"10.1093","volume":"67","author":[{"given":"Jialing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Sans","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel J","family":"DeHoog","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyana Y","family":"Garza","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary 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TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney C","family":"Povilaitis","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitesh","family":"Katta","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E","family":"Milner","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, The University of Texas at Austin , Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendong","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology, Baylor College of Medicine , Houston, 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