{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:57:56Z","timestamp":1791215876049,"version":"4.1.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2020A1515110075"],"award-info":[{"award-number":["2020A1515110075"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>This study was designed to investigate the association between donor-derived cell-free DNA (dd-cfDNA) and renal allograft injuries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This single-center study enrolled 113 adult kidney transplant recipients with kidney biopsies. Plasma and urine dd-cfDNA was detected by target region capture sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Plasma dd-cfDNA fraction was increased in multiple types of injuries, but most significantly in antibody-mediated rejection. Plasma dd-cfDNA fraction in isolated antibody-mediated rejection (1.94%, IQR: 1.15%, 2.33%) was higher than in T cell-mediated rejection (0.55%, IQR: 0.50%, 0.73%, P\u2009=\u20090.002) and negative biopsies (0.58%, IQR: 0.42%, 0.78%, P\u2009&amp;lt;\u20090.001), but lower than in mixed rejection (2.49%, IQR: 1.16%, 4.90%, P\u2009=\u20090.342). Increased urine dd-cfDNA concentration was associated with several types of injury, but most significantly with BK polyomavirus-associated nephropathy. Urine dd-cfDNA concentration in BK polyomavirus-associated nephropathy (12.22\u2005ng\/mL, IQR: 6.53\u2005ng\/mL, 31.66\u2005ng\/mL) was respectively higher than that in T cell-mediated rejection (5.24\u2005ng\/mL, IQR: 3.22\u2005ng\/mL, 6.99 ng\/mL, P\u2009=\u20090.001), borderline change (3.93\u2005ng\/mL, IQR: 2.45\u2005ng\/mL, 6.30\u2005ng\/mL, P\u2009&amp;lt;\u20090.001), and negative biopsies (3.09\u2005ng\/mL, IQR: 1.94\u2005ng\/mL, 5.05\u2005ng\/mL, P\u2009&amp;lt;\u20090.001). Plasma dd-cfDNA fraction was positively associated with glomerulitis (r\u2009=\u20090.365, P\u2009&amp;lt;\u20090.001) and peri-tubular capillaritis (r\u2009=\u20090.344, P\u2009&amp;lt;\u20090.001), while urine dd-cfDNA concentration correlated with tubulitis (r\u2009=\u20090.302, P\u2009=\u20090.002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both plasma and urine dd-cfDNA are sensitive markers for renal allograft injuries. The interpretation of a specific disease by dd-cfDNA should be combined with other clinical indicators.<\/jats:p><\/jats:sec>","DOI":"10.1093\/clinchem\/hvac053","type":"journal-article","created":{"date-parts":[[2022,3,18]],"date-time":"2022-03-18T12:13:10Z","timestamp":1647605590000},"page":"814-825","source":"Crossref","is-referenced-by-count":18,"title":["Using Both Plasma and Urine Donor-Derived Cell-Free DNA to Identify Various Renal Allograft Injuries"],"prefix":"10.1093","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7433-3447","authenticated-orcid":false,"given":"Xu-Tao","family":"Chen","sequence":"first","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Qiu","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi-Xuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Chen","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi-Cong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Dong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"He","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Shen","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Quan","family":"Luo","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Huang","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Xi","family":"Wang","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Zhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Lin","family":"Wu","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8111-6694","authenticated-orcid":false,"given":"Gang","family":"Huang","sequence":"additional","affiliation":[{"name":"Organ Transplant Center, The First Affiliated Hospital of Sun Yat-sen University , 58# Zhongshan Rd. 2, Guangzhou, Guangdong Province, China , 510080"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,1]]},"reference":[{"key":"2022060113231921500_hvac053-B1","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1097\/00000478-200306000-00012","article-title":"International variation in histologic grading is large, and persistent feedback does not improve reproducibility","volume":"27","author":"Furness","year":"2003","journal-title":"Am J Surg Pathol"},{"key":"2022060113231921500_hvac053-B2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3390\/jcm10020193","article-title":"Donor-derived 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transplant recipients: a prospective observational study","volume":"11","author":"Zhang","year":"2020","journal-title":"Front Immunol"},{"key":"2022060113231921500_hvac053-B9","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1111\/ajt.15289","article-title":"Early clinical experience using donor-derived cell-free DNA to detect rejection in kidney transplant recipients","volume":"19","author":"Huang","year":"2019","journal-title":"Am J Transplant"},{"key":"2022060113231921500_hvac053-B10","doi-asserted-by":"crossref","first-page":"2221","DOI":"10.1681\/ASN.2016091034","article-title":"Cell-free DNA and active rejection in kidney allografts","volume":"28","author":"Bloom","year":"2017","journal-title":"J Am Soc Nephrol"},{"key":"2022060113231921500_hvac053-B11","doi-asserted-by":"crossref","first-page":"3087","DOI":"10.1111\/ajt.15416","article-title":"Absolute quantification of donor-derived cell-free DNA as a marker of rejection and graft injury in kidney transplantation: 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