{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T21:40:27Z","timestamp":1789681227444,"version":"4.0.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T00:00:00Z","timestamp":1597708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Faculty of Medicine, University of Szeged"},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["GINOP-2.3.2-15-2016-00039"],"award-info":[{"award-number":["GINOP-2.3.2-15-2016-00039"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["GINOP-2.3.2-15-2016-00048"],"award-info":[{"award-number":["GINOP-2.3.2-15-2016-00048"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["K119992"],"award-info":[{"award-number":["K119992"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["EFOP-3.6.1-16-2016-00008"],"award-info":[{"award-number":["EFOP-3.6.1-16-2016-00008"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Human Capacities Hungary","award":["20391-3\/2018\/FEKUSTRAT"],"award-info":[{"award-number":["20391-3\/2018\/FEKUSTRAT"]}]},{"name":"J\u00e1nos Bolyai Research Scholarship of the Hungarian Academy of Sciences"},{"name":"Faculty of Medicine, University of Szeged"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Subunit interactions at the cytoplasmic domain interface (CD-I) have recently been shown to control gating in inward rectifier potassium channels. Here we report the novel KCNJ2 variant p.Glu293Lys that has been found in a patient with Andersen\u2013Tawil syndrome type 1 (ATS1), causing amino acid substitution at the CD-I of the inward rectifier potassium channel subunit Kir2.1. Neither has the role of Glu293 in gating control been investigated nor has a pathogenic variant been described at this position. This study aimed to assess the involvement of Glu293 in CD-I subunit interactions and to establish the pathogenic role of the p.Glu293Lys variant in ATS1.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>The p.Glu293Lys variant produced no current in homomeric form and showed dominant-negative effect over wild-type (WT) subunits. Immunocytochemical labelling showed the p.Glu293Lys subunits to distribute in the subsarcolemmal space. Salt bridge prediction indicated the presence of an intersubunit salt bridge network at the CD-I of Kir2.1, with the involvement of Glu293. Subunit interactions were studied by the NanoLuc\u00ae Binary Technology (NanoBiT) split reporter assay. Reporter constructs carrying NanoBiT tags on the intracellular termini produced no bioluminescent signal above background with the p.Glu293Lys variant in homomeric configuration and significantly reduced signals in cells co-expressing WT and p.Glu293Lys subunits simultaneously. Extracellularly presented reporter tags, however, generated comparable bioluminescent signals with heteromeric WT and p.Glu293Lys subunits and with homomeric WT channels.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Loss of function and dominant-negative effect confirm the causative role of p.Glu293Lys in ATS1. Co-assembly of Kir2.1 subunits is impaired in homomeric channels consisting of p.Glu293Lys subunits and is partially rescued in heteromeric complexes of WT and p.Glu293Lys Kir2.1 variants. These data point to an important role of Glu293 in mediating subunit assembly, as well as in gating of Kir2.1 channels.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cvr\/cvaa249","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T11:22:14Z","timestamp":1597231334000},"page":"1923-1934","source":"Crossref","is-referenced-by-count":3,"title":["Impaired cytoplasmic domain interactions cause co-assembly defect and loss of function in the p.Glu293Lys <i>KNCJ2<\/i> variant isolated from an Andersen\u2013Tawil syndrome patient"],"prefix":"10.1093","volume":"117","author":[{"given":"Szilvia","family":"D\u00e9ri","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, D\u00f3m t\u00e9r 12, PO Box 427, Szeged 6720, Hungary"},{"name":"Department of Pharmacology and Pharmacotherapy, Interdisciplinary Excellence Centre, University of Szeged, D\u00f3m t\u00e9r 12, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1nos","family":"Borb\u00e1s","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine and Cardiology Centre, University of Szeged, Semmelweis u. 8, 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teod\u00f3ra","family":"Hartai","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, D\u00f3m t\u00e9r 12, PO Box 427, Szeged 6720, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Hategan","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine and Cardiology Centre, University of Szeged, Semmelweis u. 8, 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Be\u00e1ta","family":"Cs\u00e1nyi","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine and Cardiology Centre, University of Szeged, Semmelweis u. 8, 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1d\u00e1m","family":"Visnyovszki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, D\u00f3m t\u00e9r 12, PO Box 427, Szeged 6720, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Mad\u00e1csy","sequence":"additional","affiliation":[{"name":"1st Department of Internal Medicine, University of Szeged, Kor\u00e1nyi fasor 8-10, 6720 Szeged, Hungary, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Mal\u00e9th","sequence":"additional","affiliation":[{"name":"1st Department of Internal Medicine, University of Szeged, Kor\u00e1nyi fasor 8-10, 6720 Szeged, Hungary, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Heged\u0171s","sequence":"additional","affiliation":[{"name":"Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, Temesv\u00e1ri krt. 62, 6726 Szeged, Hungary"},{"name":"Department of Biochemistry and Medical Chemistry, University of P\u00e9cs, Szigeti \u00fat 12, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2528-9173","authenticated-orcid":false,"given":"Istv\u00e1n","family":"Nagy","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry, Biological Research Centre the Hungarian Academy of Sciences, Temesv\u00e1ri krt. 62, 6726 Szeged, Hungary"},{"name":"Seqomics Biotechnology Ltd, V\u00e1llalkoz\u00f3k \u00fatja 7, 6782 M\u00f3rahalom, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4670-4447","authenticated-orcid":false,"given":"Rohit","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain J","family":"Labro","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium"},{"name":"Department of Basic Medical Sciences, University of Ghent, C. Heymanslaan 10, 9000 Ghent, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"K\u00f6rnyei","sequence":"additional","affiliation":[{"name":"Gottsegen Gy\u00f6rgy National Institute of Cardiology, Haller u. 9, 1096 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e1s","family":"Varr\u00f3","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, D\u00f3m t\u00e9r 12, PO Box 427, Szeged 6720, Hungary"},{"name":"Department of Pharmacology and Pharmacotherapy, Interdisciplinary Excellence Centre, University of Szeged, D\u00f3m t\u00e9r 12, 6720 Szeged, Hungary"},{"name":"MTA-SZTE Research Group for Cardiovascular Pharmacology, Hungarian Academy of Sciences, D\u00f3m t\u00e9r 12, 6720 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4964-1661","authenticated-orcid":false,"given":"R\u00f3bert","family":"Sepp","sequence":"additional","affiliation":[{"name":"2nd Department of Internal Medicine and Cardiology Centre, University of Szeged, Semmelweis u. 8, 6725 Szeged, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-3079","authenticated-orcid":false,"given":"Bal\u00e1zs","family":"\u00d6rd\u00f6g","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pharmacotherapy, University of Szeged, D\u00f3m t\u00e9r 12, PO Box 427, Szeged 6720, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,8,18]]},"reference":[{"key":"2021070715551624400_cvaa249-B1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1111\/j.1651-2227.1971.tb06990.x","article-title":"Intermittent muscular weakness, extrasystoles, and multiple developmental anomalies. A new syndrome?","volume":"60","author":"Andersen","year":"1971","journal-title":"Acta Paediatr Scand"},{"key":"2021070715551624400_cvaa249-B2","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/ana.410350313","article-title":"Andersen's syndrome: potassium-sensitive periodic paralysis, ventricular ectopy, and dysmorphic features","volume":"35","author":"Tawil","year":"1994","journal-title":"Ann Neurol"},{"key":"2021070715551624400_cvaa249-B3","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1002\/ajmg.a.31092","article-title":"Andersen-Tawil syndrome: prospective cohort analysis and expansion of the phenotype","volume":"140A","author":"Yoon","year":"2006","journal-title":"Am J Med Genet A"},{"key":"2021070715551624400_cvaa249-B4","doi-asserted-by":"crossref","first-page":"2720","DOI":"10.1161\/CIRCULATIONAHA.104.472498","article-title":"Electrocardiographic features in Andersen-Tawil syndrome patients with KCNJ2 mutations: characteristic T-U-wave patterns predict the KCNJ2 genotype","volume":"111","author":"Zhang","year":"2005","journal-title":"Circulation"},{"key":"2021070715551624400_cvaa249-B5","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1093\/europace\/eut160","article-title":"Cardiac characteristics and long-term outcome in Andersen-Tawil syndrome patients related to KCNJ2 mutation","volume":"15","author":"Delannoy","year":"2013","journal-title":"Europace"},{"key":"2021070715551624400_cvaa249-B6","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0092-8674(01)00342-7","article-title":"Mutations in Kir2.1 cause the developmental and episodic electrical phenotypes of Andersen's syndrome","volume":"105","author":"Plaster","year":"2001","journal-title":"Cell"},{"key":"2021070715551624400_cvaa249-B7","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.yjmcc.2009.08.013","article-title":"Cardiac strong inward rectifier potassium channels","volume":"48","author":"Anumonwo","year":"2010","journal-title":"J Mol Cell Cardiol"},{"key":"2021070715551624400_cvaa249-B8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.hrthm.2004.11.012","article-title":"The inward rectifier current (IK1) controls cardiac excitability and is involved in arrhythmogenesis","volume":"2","author":"Dhamoon","year":"2005","journal-title":"Heart Rhythm"},{"key":"2021070715551624400_cvaa249-B9","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1113\/jphysiol.2014.283648","article-title":"Inward rectifier potassium currents in mammalian skeletal muscle fibres","volume":"593","author":"DiFranco","year":"2015","journal-title":"J Physiol"},{"key":"2021070715551624400_cvaa249-B10","doi-asserted-by":"crossref","first-page":"9526","DOI":"10.1074\/jbc.M110.170597","article-title":"Kir2.6 regulates the surface expression of Kir2.x inward rectifier potassium channels","volume":"286","author":"Dassau","year":"2011","journal-title":"J Biol Chem"},{"key":"2021070715551624400_cvaa249-B11","doi-asserted-by":"crossref","first-page":"C876","DOI":"10.1152\/ajpcell.00519.2008","article-title":"Mechanisms underlying Andersen's syndrome pathology in skeletal muscle are revealed in human myotubes","volume":"297","author":"Sacconi","year":"2009","journal-title":"Am J Physiol Cell Physiol"},{"key":"2021070715551624400_cvaa249-B12","doi-asserted-by":"crossref","first-page":"3245","DOI":"10.1113\/JP271930","article-title":"Bioelectric signalling via potassium channels: a mechanism for craniofacial dysmorphogenesis in KCNJ2-associated Andersen-Tawil Syndrome","volume":"594","author":"Adams","year":"2016","journal-title":"J Physiol"},{"key":"2021070715551624400_cvaa249-B13","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.hrthm.2005.11.026","article-title":"Cellular basis for electrocardiographic and arrhythmic manifestations of Andersen-Tawil syndrome (LQT7)","volume":"3","author":"Tsuboi","year":"2006","journal-title":"Heart Rhythm"},{"key":"2021070715551624400_cvaa249-B14","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1016\/j.jacc.2020.02.033","article-title":"Natural history and risk stratification in Andersen-Tawil syndrome type 1","volume":"75","author":"Mazzanti","year":"2020","journal-title":"J Am Coll Cardiol"},{"key":"2021070715551624400_cvaa249-B15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijcard.2013.10.010","article-title":"Andersen-Tawil syndrome: clinical and molecular aspects","volume":"170","author":"Nguyen","year":"2013","journal-title":"Int J Cardiol"},{"key":"2021070715551624400_cvaa249-B16","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/S0896-6273(02)00725-0","article-title":"Alterations in conserved Kir channel-PIP2 interactions underlie channelopathies","volume":"34","author":"Lopes","year":"2002","journal-title":"Neuron"},{"key":"2021070715551624400_cvaa249-B17","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.yjmcc.2007.05.005","article-title":"T75M-KCNJ2 mutation causing Andersen-Tawil syndrome enhances inward rectification by changing Mg2+ sensitivity","volume":"43","author":"Tani","year":"2007","journal-title":"J Mol Cell Cardiol"},{"key":"2021070715551624400_cvaa249-B18","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s00395-013-0353-1","article-title":"Non dominant-negative KCNJ2 gene mutations leading to Andersen-Tawil syndrome with an isolated cardiac phenotype","volume":"108","author":"Limberg","year":"2013","journal-title":"Basic Res Cardiol"},{"key":"2021070715551624400_cvaa249-B19","doi-asserted-by":"crossref","first-page":"5781","DOI":"10.1074\/jbc.M608776200","article-title":"An Andersen-Tawil syndrome mutation in Kir2.1 (V302M) alters the G-loop cytoplasmic K+ conduction pathway","volume":"282","author":"Ma","year":"2007","journal-title":"J Biol Chem"},{"key":"2021070715551624400_cvaa249-B20","doi-asserted-by":"crossref","first-page":"8599","DOI":"10.1021\/bi060653d","article-title":"Andersen's syndrome mutation effects on the structure and assembly of the cytoplasmic domains of Kir2.1","volume":"45","author":"Pegan","year":"2006","journal-title":"Biochemistry"},{"key":"2021070715551624400_cvaa249-B21","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nn1411","article-title":"Cytoplasmic domain structures of Kir2.1 and Kir3.1 show sites for modulating gating and rectification","volume":"8","author":"Pegan","year":"2005","journal-title":"Nat Neurosci"},{"key":"2021070715551624400_cvaa249-B22","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1085\/jgp.201611719","article-title":"Control of Kir channel gating by cytoplasmic domain interface interactions","volume":"149","author":"Borschel","year":"2017","journal-title":"J Gen Physiol"},{"key":"2021070715551624400_cvaa249-B23","doi-asserted-by":"crossref","first-page":"10087","DOI":"10.1074\/jbc.M117.785154","article-title":"Conformational changes at cytoplasmic intersubunit interactions control Kir channel gating","volume":"292","author":"Wang","year":"2017","journal-title":"J Biol Chem"},{"key":"2021070715551624400_cvaa249-B24","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nsmb.3138","article-title":"Structural dynamics of potassium-channel gating revealed by single-molecule FRET","volume":"23","author":"Wang","year":"2016","journal-title":"Nat Struct Mol Biol"},{"key":"2021070715551624400_cvaa249-B25","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.ijcard.2017.04.058","article-title":"A novel \u2018splice site\u2019 HCN4 Gene mutation, c.1737\u2009+\u20091 G&gt;T, causes familial bradycardia, reduced heart rate response, impaired chronotropic competence and increased short-term heart rate variability","volume":"241","author":"Hategan","year":"2017","journal-title":"Int J Cardiol"},{"key":"2021070715551624400_cvaa249-B26","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1139\/cjpp-2014-0527","article-title":"Identification and functional characterisation of a novel KCNJ2 mutation, Val302del, causing Andersen-Tawil syndrome","volume":"93","author":"\u00d6rd\u00f6g","year":"2015","journal-title":"Can J Physiol Pharmacol"},{"key":"2021070715551624400_cvaa249-B27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","article-title":"Site-directed mutagenesis by overlap extension using the polymerase chain reaction","volume":"77","author":"Ho","year":"1989","journal-title":"Gene"},{"key":"2021070715551624400_cvaa249-B28","doi-asserted-by":"crossref","first-page":"e73255","DOI":"10.1371\/journal.pone.0073255","article-title":"Snorkel: an epitope tagging system for measuring the surface expression of membrane proteins","volume":"8","author":"Brown","year":"2013","journal-title":"PLoS One"},{"key":"2021070715551624400_cvaa249-B29","doi-asserted-by":"crossref","first-page":"137","DOI":"10.6026\/97320630003137","article-title":"ESBRI: a web server for evaluating salt bridges in proteins","volume":"3","author":"Costantini","year":"2008","journal-title":"Bioinformation"},{"key":"2021070715551624400_cvaa249-B30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nature10370","article-title":"Structural basis of PIP2 activation of the classical inward rectifier K+ channel Kir2.2","volume":"477","author":"Hansen","year":"2011","journal-title":"Nature"},{"key":"2021070715551624400_cvaa249-B31","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1038\/gim.2015.30","article-title":"Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology","volume":"17","author":"Richards","year":"2015","journal-title":"Genet Med"},{"key":"2021070715551624400_cvaa249-B32","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1172\/JCI15183","article-title":"Functional and clinical characterization of KCNJ2 mutations associated with LQT7 (Andersen syndrome)","volume":"110","author":"Tristani-Firouzi","year":"2002","journal-title":"J Clin Invest"},{"key":"2021070715551624400_cvaa249-B33","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1021\/acschembio.5b00753","article-title":"NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells","volume":"11","author":"Dixon","year":"2016","journal-title":"ACS Chem Biol"},{"key":"2021070715551624400_cvaa249-B34","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.nmd.2016.11.006","article-title":"Intrafamilial phenotypic variability in Andersen-Tawil syndrome: a diagnostic challenge in a potentially treatable condition","volume":"27","author":"Ardissone","year":"2017","journal-title":"Neuromuscul Disord"},{"key":"2021070715551624400_cvaa249-B35","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1093\/europace\/eut326","article-title":"Andersen-Tawil syndrome, scarier for the doctor than for the patient? Who, when, and how to treat","volume":"15","author":"Wilde","year":"2013","journal-title":"Europace"},{"key":"2021070715551624400_cvaa249-B36","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.bcp.2018.01.008","article-title":"Molecular dissection of the human A3 adenosine receptor coupling with beta-arrestin2","volume":"148","author":"Storme","year":"2018","journal-title":"Biochem Pharmacol"},{"key":"2021070715551624400_cvaa249-B37","doi-asserted-by":"crossref","first-page":"8750","DOI":"10.1074\/jbc.RA117.001045","article-title":"Quantitative interaction analysis permits molecular insights into functional NOX4 NADPH oxidase heterodimer assembly","volume":"293","author":"O'Neill","year":"2018","journal-title":"J Biol Chem"},{"key":"2021070715551624400_cvaa249-B38","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.ab.2018.05.002","article-title":"Antibody-free detection of cellular neddylation dynamics of Cullin1","volume":"555","author":"Schwinn","year":"2018","journal-title":"Anal Biochem"},{"key":"2021070715551624400_cvaa249-B39","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1111\/j.1749-6632.1999.tb11293.x","article-title":"Molecular diversity of K+ channels","volume":"868","author":"Coetzee","year":"1999","journal-title":"Annals NY Acad Sci"},{"key":"2021070715551624400_cvaa249-B40","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3389\/fchem.2014.00009","article-title":"Allosteric modulation of protein oligomerization: an emerging approach to drug design","volume":"2","author":"Gabizon","year":"2014","journal-title":"Front Chem"},{"key":"2021070715551624400_cvaa249-B41","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1080\/17460441.2017.1346608","article-title":"Stabilization of protein-protein interactions in drug discovery","volume":"12","author":"Andrei","year":"2017","journal-title":"Expert Opin Drug Discov"},{"key":"2021070715551624400_cvaa249-B42","doi-asserted-by":"crossref","first-page":"15631","DOI":"10.1073\/pnas.1004021107","article-title":"Flecainide increases Kir2.1 currents by interacting with cysteine 311, decreasing the polyamine-induced rectification","volume":"107","author":"Caballero","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"2021070715551624400_cvaa249-B43","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1093\/cvr\/cvu203","article-title":"Structural basis of drugs that increase cardiac inward rectifier Kir2.1 currents","volume":"104","author":"G\u00f3mez","year":"2014","journal-title":"Cardiovasc Res"},{"key":"2021070715551624400_cvaa249-B44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1097\/FJC.0b013e3182350bcc","article-title":"A novel discovery of IK1 channel agonist: zacopride selectively enhances IK1 current and suppresses triggered arrhythmias in the rat","volume":"59","author":"Liu","year":"2012","journal-title":"J Cardiovasc Pharmacol"}],"container-title":["Cardiovascular Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/cardiovascres\/advance-article-pdf\/doi\/10.1093\/cvr\/cvaa249\/34659076\/cvaa249.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/cardiovascres\/article-pdf\/117\/8\/1923\/38878758\/cvaa249.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/cardiovascres\/article-pdf\/117\/8\/1923\/38878758\/cvaa249.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,7]],"date-time":"2021-07-07T20:04:02Z","timestamp":1625688242000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article\/117\/8\/1923\/5893951"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,18]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,8,18]]},"published-print":{"date-parts":[[2021,7,7]]}},"URL":"https:\/\/doi.org\/10.1093\/cvr\/cvaa249","relation":{},"ISSN":["0008-6363","1755-3245"],"issn-type":[{"value":"0008-6363","type":"print"},{"value":"1755-3245","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,7,1]]},"published":{"date-parts":[[2020,8,18]]}}}