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Such plasticity underlies blood vessel homeostasis and contributes to vascular disease development. Oligoclonal VSMC contribution is a hallmark of end-stage vascular disease. Here, we aim to understand cellular mechanisms underpinning generation of this VSMC oligoclonality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>We investigate the dynamics of VSMC clone formation using confocal microscopy and single-cell transcriptomics in VSMC-lineage-traced animal models. We find that activation of medial VSMC proliferation occurs at low frequency after vascular injury and that only a subset of expanding clones migrate, which together drives formation of oligoclonal neointimal lesions. VSMC contribution in small atherosclerotic lesions is typically from one or two clones, similar to observations in mature lesions. Low frequency (&amp;lt;0.1%) of clonal VSMC proliferation is also observed in vitro. Single-cell RNA-sequencing revealed progressive cell state changes across a contiguous VSMC population at onset of injury-induced proliferation. Proliferating VSMCs mapped selectively to one of two distinct trajectories and were associated with cells showing extensive phenotypic switching. A proliferation-associated transitory state shared pronounced similarities with atypical SCA1+ VSMCs from uninjured mouse arteries and VSMCs in healthy human aorta. We show functionally that clonal expansion of SCA1+ VSMCs from healthy arteries occurs at higher rate and frequency compared with SCA1\u2212 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our data suggest that activation of proliferation at low frequency is a general, cell-intrinsic feature of VSMCs. We show that rare VSMCs in healthy arteries display VSMC phenotypic switching akin to that observed in pathological vessel remodelling and that this is a conserved feature of mouse and human healthy arteries. The increased proliferation of modulated VSMCs from healthy arteries suggests that these cells respond more readily to disease-inducing cues and could drive oligoclonal VSMC expansion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cvr\/cvac138","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T11:19:20Z","timestamp":1661167160000},"page":"1279-1294","source":"Crossref","is-referenced-by-count":57,"title":["Cellular mechanisms of oligoclonal vascular smooth muscle cell expansion in cardiovascular disease"],"prefix":"10.1093","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4391-5377","authenticated-orcid":false,"given":"Matt D","family":"Worssam","sequence":"first","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4724-5200","authenticated-orcid":false,"given":"Jordi","family":"Lambert","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3476-3359","authenticated-orcid":false,"given":"Sebnem","family":"Oc","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C K","family":"Taylor","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel L","family":"Taylor","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Dobnikar","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"},{"name":"Babraham Institute , Cambridge , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-4100","authenticated-orcid":false,"given":"Joel","family":"Chappell","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7678-177X","authenticated-orcid":false,"given":"Jennifer L","family":"Harman","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichola L","family":"Figg","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-9474","authenticated-orcid":false,"given":"Alison","family":"Finigan","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty","family":"Foote","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K","family":"Uryga","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University of Cambridge , Papworth Road, Cambridge Biomedical Campus, Cambridge CB2 0BB , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2565-1825","authenticated-orcid":false,"given":"Martin R","family":"Bennett","sequence":"additional","affiliation":[{"name":"Section of Cardiorespiratory Medicine, Department of Medicine, University 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