{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T20:14:13Z","timestamp":1790280853204,"version":"4.1.0"},"reference-count":77,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"British Heart Foundation fellowship","award":["FS\/17\/57\/32934"],"award-info":[{"award-number":["FS\/17\/57\/32934"]}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["RE\/18\/2\/34213"],"award-info":[{"award-number":["RE\/18\/2\/34213"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["PG\/20\/6\/34835"],"award-info":[{"award-number":["PG\/20\/6\/34835"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["MR\/S023593\/1"],"award-info":[{"award-number":["MR\/S023593\/1"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"name":"British Heart Foundation Chair","award":["CH\/1999001\/11735"],"award-info":[{"award-number":["CH\/1999001\/11735"]}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","award":["BB\/S001123\/1"],"award-info":[{"award-number":["BB\/S001123\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["GM028521"],"award-info":[{"award-number":["GM028521"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Nuclear envelope integrity is essential for the compartmentalization of the nucleus and cytoplasm. Importantly, mutations in genes encoding nuclear envelope (NE) and associated proteins are the second highest cause of familial dilated cardiomyopathy. One such NE protein that causes cardiomyopathy in humans and affects mouse heart development is Lem2. However, its role in the heart remains poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>We generated mice in which Lem2 was specifically ablated either in embryonic cardiomyocytes (Lem2 cKO) or in adult cardiomyocytes (Lem2 iCKO) and carried out detailed physiological, tissue, and cellular analyses. High-resolution episcopic microscopy was used for three-dimensional reconstructions and detailed morphological analyses. RNA-sequencing and immunofluorescence identified altered pathways and cellular phenotypes, and cardiomyocytes were isolated to interrogate nuclear integrity in more detail. In addition, echocardiography provided a physiological assessment of Lem2 iCKO adult mice. We found that Lem2 was essential for cardiac development, and hearts from Lem2 cKO mice were morphologically and transcriptionally underdeveloped. Lem2 cKO hearts displayed high levels of DNA damage, nuclear rupture, and apoptosis. Crucially, we found that these defects were driven by muscle contraction as they were ameliorated by inhibiting myosin contraction and L-type calcium channels. Conversely, reducing Lem2 levels to \u223c45% in adult cardiomyocytes did not lead to overt cardiac dysfunction up to 18 months of age.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data suggest that Lem2 is critical for integrity at the nascent NE in foetal hearts, and protects the nucleus from the mechanical forces of muscle contraction. In contrast, the adult heart is not detectably affected by partial Lem2 depletion, perhaps owing to a more established NE and increased adaptation to mechanical stress. Taken together, these data provide insights into mechanisms underlying cardiomyopathy in patients with mutations in Lem2 and cardio-laminopathies in general.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cvr\/cvad061","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T09:15:35Z","timestamp":1681722935000},"page":"2074-2088","source":"Crossref","is-referenced-by-count":17,"title":["Lem2 is essential for cardiac development by maintaining nuclear integrity"],"prefix":"10.1093","volume":"119","author":[{"given":"Jacob A","family":"Ross","sequence":"first","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaly","family":"Arcos-Villacis","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edmund","family":"Battey","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"},{"name":"Centre of Human and Applied Physiological Sciences, School of Basic and Medical Biosciences, Faculty of Life Sciences and Medicine, King\u2019s College London , London SE1 1UL , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis","family":"Boogerd","sequence":"additional","affiliation":[{"name":"Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW), University Medical Center Utrecht , Utrecht 3584 CT , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constanza Avalos","family":"Orellana","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Marhuenda","sequence":"additional","affiliation":[{"name":"Division of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London , London E1 4NS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Swiatlowska","sequence":"additional","affiliation":[{"name":"Division of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London , London E1 4NS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Hodzic","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Washington University School of Medicine , 660S. Euclid Avenue, St Louis, MO 63110 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrice","family":"Prin","sequence":"additional","affiliation":[{"name":"Crick Advanced Light Microscopy Facility, The Francis Crick Institute , London NW1 1AT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Mohun","sequence":"additional","affiliation":[{"name":"Crick Advanced Light Microscopy Facility, The Francis Crick Institute , London NW1 1AT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman","family":"Catibog","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Tapia","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, The Scripps Research Institute , La Jolla, CA 92037 , USA"},{"name":"Research Group on Foods, Nutritional Biochemistry and Health, Universidad Europea del Atla\u0301ntico , Santander 39011 , Spain"},{"name":"Department of Basic Medical Sciences, Institute of Biomedical Technologies, University of La Laguna , Tenerife 38200 , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larry","family":"Gerace","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, The Scripps Research Institute , La Jolla, CA 92037 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Iskratsch","sequence":"additional","affiliation":[{"name":"Division of Bioengineering, School of Engineering and Materials Science, Queen Mary University of London , London E1 4NS , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay M","family":"Shah","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3337-6851","authenticated-orcid":false,"given":"Matthew J","family":"Stroud","sequence":"additional","affiliation":[{"name":"British Heart Foundation Centre of Research Excellence, School of Cardiovascular & Metabolic Medicine & Sciences, Faculty of Life Sciences & Medicine, King\u2019s College London , James Black Centre, 125 Coldharbour Lane, London SE5 9NU , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"2023090513412141700_cvad061-B1","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1083\/jcb.79.2.546","article-title":"Immunocytochemical localization of the major polypeptides of the nuclear pore complex-lamina fraction. Interphase and mitotic distribution","volume":"79","author":"Gerace","year":"1978","journal-title":"J Cell Biol"},{"key":"2023090513412141700_cvad061-B2","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1111\/j.1582-4934.2008.00676.x","article-title":"Nuclear lamins: key regulators of nuclear structure and activities","volume":"13","author":"Prokocimer","year":"2009","journal-title":"J Cell Mol Med"},{"key":"2023090513412141700_cvad061-B3","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1172\/JCI200419670","article-title":"Lamin A\/C deficiency causes defective nuclear mechanics and mechanotransduction","volume":"113","author":"Lammerding","year":"2004","journal-title":"J Clin Invest"},{"key":"2023090513412141700_cvad061-B4","doi-asserted-by":"crossref","first-page":"106035","DOI":"10.1016\/j.biocel.2021.106035","article-title":"THE NUCLEUS: mechanosensing in cardiac disease","volume":"137","author":"Ross","year":"2021","journal-title":"Int J Biochem Cell Biol"},{"key":"2023090513412141700_cvad061-B5","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1007\/s12551-018-0431-6","article-title":"Linker of nucleoskeleton and cytoskeleton complex proteins in cardiomyopathy","volume":"10","author":"Stroud","year":"2018","journal-title":"Biophys Rev"},{"key":"2023090513412141700_cvad061-B6","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1083\/jcb.200509124","article-title":"Coupling of the nucleus and cytoplasm: role of the LINC complex","volume":"172","author":"Crisp","year":"2006","journal-title":"J Cell Biol"},{"key":"2023090513412141700_cvad061-B7","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1083\/jcb.201612128","article-title":"Nesprin 1alpha2 is essential for mouse postnatal viability and nuclear positioning in skeletal muscle","volume":"216","author":"Stroud","year":"2017","journal-title":"J Cell Biol"},{"key":"2023090513412141700_cvad061-B8","doi-asserted-by":"crossref","first-page":"e1004114","DOI":"10.1371\/journal.pgen.1004114","article-title":"Targeted ablation of nesprin 1 and nesprin 2 from murine myocardium results in cardiomyopathy, altered nuclear morphology and inhibition of the biomechanical gene response","volume":"10","author":"Banerjee","year":"2014","journal-title":"PLoS Genet"},{"key":"2023090513412141700_cvad061-B9","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1161\/CIRCRESAHA.114.301236","article-title":"Linker of nucleoskeleton and cytoskeleton complex proteins in cardiac structure, function, and disease","volume":"114","author":"Stroud","year":"2014","journal-title":"Circ Res"},{"key":"2023090513412141700_cvad061-B10","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/hmg\/ddn386","article-title":"Disruption of nesprin-1 produces an Emery Dreifuss muscular dystrophy-like phenotype in mice","volume":"18","author":"Puckelwartz","year":"2009","journal-title":"Hum Mol Genet"},{"key":"2023090513412141700_cvad061-B11","doi-asserted-by":"crossref","first-page":"4722","DOI":"10.1038\/s41467-021-24849-4","article-title":"Disrupting the LINC complex by AAV mediated gene transduction prevents progression of Lamin induced cardiomyopathy","volume":"12","author":"Chai","year":"2021","journal-title":"Nat Commun"},{"key":"2023090513412141700_cvad061-B12","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1091\/mbc.E18-07-0438","article-title":"Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis","volume":"30","author":"Stewart","year":"2019","journal-title":"Mol Biol Cell"},{"key":"2023090513412141700_cvad061-B13","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/ng1294-323","article-title":"Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy","volume":"8","author":"Bione","year":"1994","journal-title":"Nat Genet"},{"key":"2023090513412141700_cvad061-B14","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1002\/humu.20250","article-title":"Thymopoietin (lamina-associated polypeptide 2) gene mutation associated with dilated cardiomyopathy","volume":"26","author":"Taylor","year":"2005","journal-title":"Hum Mutat"},{"key":"2023090513412141700_cvad061-B15","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/mgg3.181","article-title":"Hutterite-type cataract maps to chromosome 6p21.32-p21.31, cosegregates with a homozygous mutation in LEMD2, and is associated with sudden cardiac death","volume":"4","author":"Boone","year":"2016","journal-title":"Mol Genet Genomic Med"},{"key":"2023090513412141700_cvad061-B16","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.jacbts.2018.12.001","article-title":"Characterization of a unique form of arrhythmic cardiomyopathy caused by recessive mutation in LEMD2","volume":"4","author":"Abdelfatah","year":"2019","journal-title":"JACC Basic Transl Sci"},{"key":"2023090513412141700_cvad061-B17","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1016\/j.ajhg.2019.02.021","article-title":"The discovery of a LEMD2-associated nuclear envelopathy with early progeroid appearance suggests advanced applications for AI-driven facial phenotyping","volume":"104","author":"Marbach","year":"2019","journal-title":"Am J Hum Genet"},{"key":"2023090513412141700_cvad061-B18","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1083\/jcb.201901116","article-title":"Repair of nuclear ruptures requires barrier-to-autointegration factor","volume":"218","author":"Halfmann","year":"2019","journal-title":"J Cell Biol"},{"key":"2023090513412141700_cvad061-B19","doi-asserted-by":"crossref","first-page":"E2166","DOI":"10.1073\/pnas.1613916114","article-title":"LEM2 recruits CHMP7 for ESCRT-mediated nuclear envelope closure in fission yeast and human cells","volume":"114","author":"Gu","year":"2017","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023090513412141700_cvad061-B20","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1038\/s41556-020-0537-5","article-title":"Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation","volume":"22","author":"Vietri","year":"2020","journal-title":"Nat Cell Biol"},{"key":"2023090513412141700_cvad061-B21","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1038\/s41586-020-2232-x","article-title":"LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation","volume":"582","author":"von Appen","year":"2020","journal-title":"Nature"},{"key":"2023090513412141700_cvad061-B22","doi-asserted-by":"crossref","first-page":"6435","DOI":"10.1016\/j.febslet.2006.10.060","article-title":"The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology","volume":"580","author":"Ulbert","year":"2006","journal-title":"FEBS Lett"},{"key":"2023090513412141700_cvad061-B23","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1101\/gad.271288.115","article-title":"Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2","volume":"30","author":"Barrales","year":"2016","journal-title":"Genes Dev"},{"key":"2023090513412141700_cvad061-B24","first-page":"1090","article-title":"Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans","volume":"128","author":"Morales-Martinez","year":"2015","journal-title":"J Cell Sci"},{"key":"2023090513412141700_cvad061-B25","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1016\/j.cell.2012.06.051","article-title":"Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery","volume":"150","author":"Towbin","year":"2012","journal-title":"Cell"},{"key":"2023090513412141700_cvad061-B26","doi-asserted-by":"crossref","first-page":"e59999","DOI":"10.7554\/eLife.59999","article-title":"CDK1 controls CHMP7-dependent nuclear envelope reformation","volume":"10","author":"Gatta","year":"2021","journal-title":"Elife"},{"key":"2023090513412141700_cvad061-B27","doi-asserted-by":"crossref","first-page":"e0116196","DOI":"10.1371\/journal.pone.0116196","article-title":"Nuclear envelope protein Lem2 is required for mouse development and regulates MAP and AKT kinases","volume":"10","author":"Tapia","year":"2015","journal-title":"PLoS One"},{"key":"2023090513412141700_cvad061-B28","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1038\/nature10163","article-title":"A conditional knockout resource for the genome-wide study of mouse gene function","volume":"474","author":"Skarnes","year":"2011","journal-title":"Nature"},{"key":"2023090513412141700_cvad061-B29","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.cell.2013.06.022","article-title":"Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes","volume":"154","author":"White","year":"2013","journal-title":"Cell"},{"key":"2023090513412141700_cvad061-B30","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1002\/1526-968X(200011\/12)28:3\/4<106::AID-GENE30>3.0.CO;2-T","article-title":"Widespread recombinase expression using FLPeR (flipper) mice","volume":"28","author":"Farley","year":"2000","journal-title":"Genesis"},{"key":"2023090513412141700_cvad061-B31","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/dvg.20275","article-title":"Pan-myocardial expression of Cre recombinase throughout mouse development","volume":"45","author":"Breckenridge","year":"2007","journal-title":"Genesis"},{"key":"2023090513412141700_cvad061-B32","doi-asserted-by":"crossref","first-page":"2362","DOI":"10.1101\/gad.1124803","article-title":"An essential role of Bmp4 in the atrioventricular septation of the mouse heart","volume":"17","author":"Jiao","year":"2003","journal-title":"Genes Dev"},{"key":"2023090513412141700_cvad061-B33","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1002\/dvg.22861","article-title":"Generation of a tamoxifen inducible Tnnt2MerCreMer knock-in mouse model for cardiac studies","volume":"53","author":"Yan","year":"2015","journal-title":"Genesis"},{"key":"2023090513412141700_cvad061-B34","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1093\/cvr\/cvx205","article-title":"Luma is not essential for murine cardiac development and function","volume":"114","author":"Stroud","year":"2018","journal-title":"Cardiovasc Res"},{"key":"2023090513412141700_cvad061-B35","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1172\/JCI80335","article-title":"Normalization of Naxos plakoglobin levels restores cardiac function in mice","volume":"125","author":"Zhang","year":"2015","journal-title":"J Clin Invest"},{"key":"2023090513412141700_cvad061-B36","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1161\/CIRCRESAHA.116.309202","article-title":"A simplified, Langendorff-free method for concomitant isolation of viable cardiac myocytes and nonmyocytes from the adult mouse heart","volume":"119","author":"Ackers-Johnson","year":"2016","journal-title":"Circ Res"},{"key":"2023090513412141700_cvad061-B37","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/978-1-4939-8691-0_18","article-title":"Generation and analysis of striated muscle selective LINC complex protein mutant mice","volume":"1840","author":"Stroud","year":"2018","journal-title":"Methods Mol Biol"},{"key":"2023090513412141700_cvad061-B38","doi-asserted-by":"crossref","first-page":"24987","DOI":"10.1074\/jbc.M111.242263","article-title":"Characterization of G2L3 (GAS2-like 3), a new microtubule- and actin-binding protein related to spectraplakins","volume":"286","author":"Stroud","year":"2011","journal-title":"J Biol Chem"},{"key":"2023090513412141700_cvad061-B39","first-page":"2672","article-title":"GAS2-like proteins mediate communication between microtubules and actin through interactions with end-binding proteins","volume":"127","author":"Stroud","year":"2014","journal-title":"J Cell Sci"},{"key":"2023090513412141700_cvad061-B40","first-page":"e56071","article-title":"High-resolution episcopic microscopy (HREM) - simple and robust protocols for processing and visualizing organic materials","volume":"125","author":"Geyer","year":"2017","journal-title":"J Vis Exp"},{"key":"2023090513412141700_cvad061-B41","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1111\/joa.12465","article-title":"Morphogenesis of myocardial trabeculae in the mouse embryo","volume":"229","author":"Captur","year":"2016","journal-title":"J Anat"},{"key":"2023090513412141700_cvad061-B42","author":"Korotkevich"},{"key":"2023090513412141700_cvad061-B43","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023090513412141700_cvad061-B44","doi-asserted-by":"crossref","first-page":"eabm3471","DOI":"10.1126\/sciadv.abm3471","article-title":"Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching","volume":"8","author":"Swiatlowska","year":"2022","journal-title":"Sci Adv"},{"key":"2023090513412141700_cvad061-B45","doi-asserted-by":"crossref","DOI":"10.1113\/JP284128","article-title":"Myonuclear alterations associated with exercise are independent of age in humans","author":"Battey","year":"2023","journal-title":"J Physiol"},{"key":"2023090513412141700_cvad061-B46","doi-asserted-by":"crossref","first-page":"9629","DOI":"10.1021\/acsnano.9b04808","article-title":"Subsurface imaging of cell organelles by force microscopy","volume":"13","author":"Guerrero","year":"2019","journal-title":"ACS Nano"},{"key":"2023090513412141700_cvad061-B47","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1038\/nature19356","article-title":"High-throughput discovery of novel developmental phenotypes","volume":"537","author":"Dickinson","year":"2016","journal-title":"Nature"},{"key":"2023090513412141700_cvad061-B48","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/cvr\/cvq048","article-title":"Gadd45beta is a novel mediator of cardiomyocyte apoptosis induced by ischaemia\/hypoxia","volume":"87","author":"Kim","year":"2010","journal-title":"Cardiovasc Res"},{"key":"2023090513412141700_cvad061-B49","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1093\/cvr\/cvv219","article-title":"Gadd45gamma regulates cardiomyocyte death and post-myocardial infarction left ventricular remodelling","volume":"108","author":"Lucas","year":"2015","journal-title":"Cardiovasc Res"},{"key":"2023090513412141700_cvad061-B50","doi-asserted-by":"crossref","first-page":"33730","DOI":"10.1074\/jbc.M114.592295","article-title":"RING finger protein RNF207, a novel regulator of cardiac excitation","volume":"289","author":"Roder","year":"2014","journal-title":"J Biol Chem"},{"key":"2023090513412141700_cvad061-B51","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s13059-018-1457-6","article-title":"Exome-chip meta-analysis identifies novel loci associated with cardiac conduction, including ADAMTS6","volume":"19","author":"Prins","year":"2018","journal-title":"Genome Biol"},{"key":"2023090513412141700_cvad061-B52","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/ng0196-17","article-title":"Positional cloning of a novel potassium channel gene: KVLQT1 mutations cause cardiac arrhythmias","volume":"12","author":"Wang","year":"1996","journal-title":"Nat Genet"},{"key":"2023090513412141700_cvad061-B53","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1006\/geno.2000.6440","article-title":"A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 19q13.4: evolution and expression profile of the gamma subunit gene family","volume":"71","author":"Burgess","year":"2001","journal-title":"Genomics"},{"key":"2023090513412141700_cvad061-B54","doi-asserted-by":"crossref","first-page":"5858","DOI":"10.1074\/jbc.273.10.5858","article-title":"DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139","volume":"273","author":"Rogakou","year":"1998","journal-title":"J Biol Chem"},{"key":"2023090513412141700_cvad061-B55","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jbiomech.2009.09.014","article-title":"Mechanobiology of cardiomyocyte development","volume":"43","author":"Jacot","year":"2010","journal-title":"J Biomech"},{"key":"2023090513412141700_cvad061-B56","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.devcel.2017.12.024","article-title":"Cardiomyocytes sense matrix rigidity through a combination of muscle and non-muscle myosin contractions","volume":"44","author":"Pandey","year":"2018","journal-title":"Dev Cell"},{"key":"2023090513412141700_cvad061-B57","doi-asserted-by":"crossref","first-page":"118436","DOI":"10.1016\/j.bbamcr.2019.01.017","article-title":"Mix and (mis-)match - the mechanosensing machinery in the changing environment of the developing, healthy adult and diseased heart","volume":"1867","author":"Ward","year":"2020","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"2023090513412141700_cvad061-B58","doi-asserted-by":"crossref","first-page":"25870","DOI":"10.1073\/pnas.1917225116","article-title":"An absence of lamin B1 in migrating neurons causes nuclear membrane ruptures and cell death","volume":"116","author":"Chen","year":"2019","journal-title":"Proc Natl Acad Sci USA"},{"key":"2023090513412141700_cvad061-B59","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/s41563-019-0563-5","article-title":"Mutant lamins cause nuclear envelope rupture and DNA damage in skeletal muscle cells","volume":"19","author":"Earle","year":"2020","journal-title":"Nat Mater"},{"key":"2023090513412141700_cvad061-B60","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1126\/science.aad7611","article-title":"ESCRT III repairs nuclear envelope ruptures during cell migration to limit DNA damage and cell death","volume":"352","author":"Raab","year":"2016","journal-title":"Science"},{"key":"2023090513412141700_cvad061-B61","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1126\/science.aaw6421","article-title":"BAF restricts cGAS on nuclear DNA to prevent innate immune activation","volume":"369","author":"Guey","year":"2020","journal-title":"Science"},{"key":"2023090513412141700_cvad061-B62","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1038\/s41421-020-0162-2","article-title":"Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis","volume":"6","author":"Zhong","year":"2020","journal-title":"Cell Discov"},{"key":"2023090513412141700_cvad061-B63","doi-asserted-by":"crossref","first-page":"eabc5386.","DOI":"10.1126\/science.abc5386","article-title":"Phosphorylation and chromatin tethering prevent cGAS activation during mitosis","volume":"371","author":"Li","year":"2021","journal-title":"Science"},{"key":"2023090513412141700_cvad061-B64","doi-asserted-by":"crossref","first-page":"3192","DOI":"10.1016\/j.devcel.2021.10.022","article-title":"The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair","volume":"56","author":"Wallis","year":"2021","journal-title":"Dev Cell"},{"key":"2023090513412141700_cvad061-B65","doi-asserted-by":"crossref","first-page":"8211","DOI":"10.1002\/anie.201403540","article-title":"para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor","volume":"53","author":"Kepiro","year":"2014","journal-title":"Angew Chem Int Ed Engl"},{"key":"2023090513412141700_cvad061-B66","doi-asserted-by":"crossref","first-page":"e42144","DOI":"10.7554\/eLife.42144","article-title":"Muscle-specific stress fibers give rise to sarcomeres in cardiomyocytes","volume":"7","author":"Fenix","year":"2018","journal-title":"Elife"},{"key":"2023090513412141700_cvad061-B67","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.1016\/0022-2828(95)90019-5","article-title":"Inhibition by verapamil and diltiazem of agonist-stimulated contractile responses in mammalian ventricular cardiomyocytes","volume":"27","author":"Bell","year":"1995","journal-title":"J Mol Cell Cardiol"},{"key":"2023090513412141700_cvad061-B68","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1091\/mbc.e11-06-0505","article-title":"Ce-emerin and LEM-2: essential roles in Caenorhabditis elegans development, muscle function, and mitosis","volume":"23","author":"Barkan","year":"2012","journal-title":"Mol Biol Cell"},{"key":"2023090513412141700_cvad061-B69","doi-asserted-by":"crossref","first-page":"5718","DOI":"10.1128\/MCB.00270-09","article-title":"Overlapping functions of nuclear envelope proteins NET25 (Lem2) and emerin in regulation of extracellular signal-regulated kinase signaling in myoblast differentiation","volume":"29","author":"Huber","year":"2009","journal-title":"Mol Cell Biol"},{"key":"2023090513412141700_cvad061-B70","doi-asserted-by":"crossref","first-page":"19767","DOI":"10.1074\/jbc.M117.809897","article-title":"Sirtuin 5 is required for mouse survival in response to cardiac pressure overload","volume":"292","author":"Hershberger","year":"2017","journal-title":"J Biol Chem"},{"key":"2023090513412141700_cvad061-B71","doi-asserted-by":"crossref","first-page":"1240104","DOI":"10.1126\/science.1240104","article-title":"Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation","volume":"341","author":"Swift","year":"2013","journal-title":"Science"},{"key":"2023090513412141700_cvad061-B72","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.cell.2013.01.009","article-title":"LBR and lamin A\/C sequentially tether peripheral heterochromatin and inversely regulate differentiation","volume":"152","author":"Solovei","year":"2013","journal-title":"Cell"},{"key":"2023090513412141700_cvad061-B73","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1242\/dev.105.2.365","article-title":"Differential timing of nuclear lamin A\/C expression in the various organs of the mouse embryo and the young animal: a developmental study","volume":"105","author":"Rober","year":"1989","journal-title":"Development"},{"key":"2023090513412141700_cvad061-B74","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/0092-8674(87)90634-9","article-title":"Teratocarcinoma stem cells and early mouse embryos contain only a single major lamin polypeptide closely resembling lamin B","volume":"51","author":"Stewart","year":"1987","journal-title":"Cell"},{"key":"2023090513412141700_cvad061-B75","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.1038\/s41467-019-11923-1","article-title":"Nuclear decoupling is part of a rapid protein-level cellular response to high-intensity mechanical loading","volume":"10","author":"Gilbert","year":"2019","journal-title":"Nat Commun"},{"key":"2023090513412141700_cvad061-B76","doi-asserted-by":"crossref","first-page":"e13152","DOI":"10.1111\/acel.13152","article-title":"Cytoskeleton stiffness regulates cellular senescence and innate immune response in Hutchinson-Gilford Progeria Syndrome","volume":"19","author":"Mu","year":"2020","journal-title":"Aging Cell"},{"key":"2023090513412141700_cvad061-B77","first-page":"H2183","article-title":"Cardiomyocyte DNA synthesis and binucleation during murine development","volume":"271","author":"Soonpaa","year":"1996","journal-title":"Am J Physiol"}],"container-title":["Cardiovascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/cardiovascres\/advance-article-pdf\/doi\/10.1093\/cvr\/cvad061\/50170672\/cvad061.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article-pdf\/119\/11\/2074\/51357663\/cvad061.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article-pdf\/119\/11\/2074\/51357663\/cvad061.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T09:53:56Z","timestamp":1693907636000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article\/119\/11\/2074\/7126317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,17]]},"references-count":77,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,4,17]]},"published-print":{"date-parts":[[2023,9,5]]}},"URL":"https:\/\/doi.org\/10.1093\/cvr\/cvad061","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.02.10.477501","asserted-by":"object"}]},"ISSN":["0008-6363","1755-3245"],"issn-type":[{"value":"0008-6363","type":"print"},{"value":"1755-3245","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,8]]},"published":{"date-parts":[[2023,4,17]]}}}