{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T19:39:10Z","timestamp":1792179550159,"version":"4.4.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Program for National Science Funds of China","award":["81730011, 82070867, 81600683, 81970721"],"award-info":[{"award-number":["81730011, 82070867, 81600683, 81970721"]}]},{"name":"Program for National Science Funds of China","award":["81927805"],"award-info":[{"award-number":["81927805"]}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2018YFA0107400"],"award-info":[{"award-number":["2018YFA0107400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018621","name":"Program for Changjiang Scholars and Innovative Research Team in University","doi-asserted-by":"publisher","award":["PCSIRT-14R08"],"award-info":[{"award-number":["PCSIRT-14R08"]}],"id":[{"id":"10.13039\/501100018621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>\u03b2II spectrin is a cytoskeletal protein known to be tightly linked to heart development and cardiovascular electrophysiology. However, the roles of \u03b2II spectrin in cardiac contractile function and pathological post-myocardial infarction remodelling remain unclear. Here, we investigated whether and how \u03b2II spectrin, the most common isoform of non-erythrocytic spectrin in cardiomyocytes, is involved in cardiac contractile function and ischaemia\/reperfusion (I\/R) injury.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>We observed that the levels of serum \u03b2II spectrin breakdown products (\u03b2II SBDPs) were significantly increased in patients with acute myocardial infarction (AMI). Concordantly, \u03b2II spectrin was degraded into \u03b2II SBDPs by calpain in mouse hearts after I\/R injury. Using tamoxifen-inducible cardiac-specific \u03b2II spectrin knockout mice, we found that deletion of \u03b2II spectrin in the adult heart resulted in spontaneous development of cardiac contractile dysfunction, cardiac hypertrophy, and fibrosis at 5 weeks after tamoxifen treatment. Moreover, at 1 week after tamoxifen treatment, although spontaneous cardiac dysfunction in cardiac-specific \u03b2II spectrin knockout mice had not developed, deletion of \u03b2II spectrin in the heart exacerbated I\/R-induced cardiomyocyte death and heart failure. Furthermore, restoration of \u03b2II spectrin expression via adenoviral small activating RNA (saRNA) delivery into the heart reduced I\/R injury. Immunoprecipitation coupled with mass spectrometry (IP\u2013LC\u2013MS\/MS) analyses and functional studies revealed that \u03b2II spectrin is indispensable for mitochondrial complex I activity and respiratory function. Mechanistically, \u03b2II spectrin promotes translocation of NADH:ubiquinone oxidoreductase 75-kDa Fe-S protein 1 (NDUFS1) from the cytosol to mitochondria by crosslinking with actin filaments (F-actin) to maintain F-actin stability.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>\u03b2II spectrin is an essential cytoskeletal element for preserving mitochondrial homeostasis and cardiac function. Defects in \u03b2II spectrin exacerbate cardiac I\/R injury.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cvr\/cvae116","type":"journal-article","created":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T01:23:42Z","timestamp":1717550622000},"page":"1312-1326","source":"Crossref","is-referenced-by-count":16,"title":["Cardiomyocyte \u03b2II spectrin plays a critical role in maintaining cardiac function by regulating mitochondrial respiratory function"],"prefix":"10.1093","volume":"120","author":[{"given":"Rongjin","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"},{"name":"Department of Cardiology, The 989th Hospital of the People\u2019s Liberation Army Joint Logistic Support Force , 2 Huaxia West Road, Luoyang 471000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banjun","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rutao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingping","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congye","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunyun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"},{"name":"Department of Cardiology, Yan\u2019an University Affiliated Hospital , 43 Bei Dajie, Yan\u2019an 716000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huishou","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjun","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Xijing Hospital, Air Force Medical University , 169 Changle West Road, Xi\u2019an 710032 , 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