{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T04:35:52Z","timestamp":1792125352746,"version":"4.4.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170236"],"award-info":[{"award-number":["82170236"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82330048"],"award-info":[{"award-number":["82330048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700212"],"award-info":[{"award-number":["81700212"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017668","name":"Key research and development projects of Anhui Province","doi-asserted-by":"publisher","award":["202104j07020037"],"award-info":[{"award-number":["202104j07020037"]}],"id":[{"id":"10.13039\/501100017668","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Excellent Youth Research Project in University of Anhui Province","award":["2023AH030116"],"award-info":[{"award-number":["2023AH030116"]}]},{"name":"Shanghai Outstanding Academic Leaders Program","award":["21XD1421700"],"award-info":[{"award-number":["21XD1421700"]}]},{"name":"The Innovation Program of The Shanghai Municipal Education Commission","award":["2023ZKZD24"],"award-info":[{"award-number":["2023ZKZD24"]}]},{"name":"Research Fund of Anhui Institute of Translational Medicine","award":["2021zhyx-C61"],"award-info":[{"award-number":["2021zhyx-C61"]}]},{"name":"Anhui Province Graduate Education Quality Engineering Project","award":["2023xscx053"],"award-info":[{"award-number":["2023xscx053"]}]},{"name":"National Natural Science Foundation Incubation Program of the Second Affiliated Hospital of Anhui Medical"},{"name":"University","award":["2020GMFY02"],"award-info":[{"award-number":["2020GMFY02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Mechanosensitive (MS) ion channels play a key role in heart development, physiology, and disease. However, little is known about the molecular mechanisms of the MS non-selective cationic channel Piezo family in cardiac fibrosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>Mice were treated with ISO\/Ang-II\/TAC to induce cardiac fibrosis. AAV9 carrying POSTN promoter-driven small hairpin RNA targeting YTHDF1, and Piezo2 were administered to ISO mice to investigate their roles in cardiac fibrosis. RNA-seq, single-cell sequencing, and histological and biochemical analyses were performed to determine the mechanism by which YTHDF1 regulates Piezo2 expression in cardiac fibrosis. Piezo2 was reconstituted in YTHDF1-deficient cardiac fibroblasts (CFs) and mouse hearts to study its effects on CF autophagy and fibrosis. Piezo2 but not Piezo1 expression increased in experimental cardiac fibrosis and TGF-\u03b21-induced CFs. Fibroblast-specific Piezo2 deficiency ameliorated fibroblast activation and autophagy and inhibited cardiac fibrosis. Mechanistically, Piezo2 up-regulation was associated with elevated m6A mRNA levels. Site-specific m6A modifications at peak_26355 were crucial for regulating the binding of YTHDF1 to Piezo2 mRNA and inducing Piezo2 translation. Notably, Piezo2 epitranscriptomic repression ameliorated experimental cardiac fibrosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We demonstrated a novel epitranscriptomic mechanism through which YTHDF1 recognizes Piezo2 and controls cardiac fibroblast autophagy and fibrosis through m6A-dependent modulation. Our findings provide new insights for the development of preventive measures for cardiac fibrosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/cvr\/cvae239","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T11:44:51Z","timestamp":1730807091000},"page":"2236-2248","source":"Crossref","is-referenced-by-count":31,"title":["Epitranscriptomic regulation of cardiac fibrosis via YTHDF1-dependent <i>PIEZO2<\/i> mRNA m6A modification"],"prefix":"10.1093","volume":"120","author":[{"given":"Ji-Fei","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Chan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute for Developmental and Regenerative Cardiovascular Medicine, MOE-Shanghai Key Laboratory of Children\u2019s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai 200092 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, The Second Affiliated Hospital of Anhui Medical University , Hefei 230601 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,","place":["China"]},{"name":"Institute for Developmental and Regenerative Cardiovascular Medicine, MOE-Shanghai Key Laboratory of Children\u2019s Environmental Health, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai 200092 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,11,5]]},"reference":[{"key":"2024123117114306100_cvae239-B1","doi-asserted-by":"crossref","first-page":"101151","DOI":"10.1016\/j.redox.2019.101151","article-title":"Vascular peroxidase 1 is a novel regulator of cardiac fibrosis after myocardial infarction","volume":"22","author":"Liu","year":"2019","journal-title":"Redox Biol"},{"key":"2024123117114306100_cvae239-B2","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.apsb.2019.04.003","article-title":"Emodin alleviates cardiac fibrosis by suppressing activation of cardiac fibroblasts via upregulating metastasis associated protein 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