{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:23:10Z","timestamp":1793128990486,"version":"4.5.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["CIHR FDN\u2014143291"],"award-info":[{"award-number":["CIHR FDN\u2014143291"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["487655"],"award-info":[{"award-number":["487655"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015099","name":"Canadian Vascular Network","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015099","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Pulmonary arterial hypertension (PAH) is a lethal pulmonary vascular disease characterized by arteriolar pruning and occlusive vascular remodelling leading to increased pulmonary vascular resistance and eventually right heart failure. While endothelial cell (EC) injury and apoptosis are known triggers for this disease, the mechanisms by which they lead to complex arterial remodelling remain obscure. We employed multiplexed single-cell RNA sequencing at multiple timepoints during the onset and progression of disease in a model of severe PAH to identify mechanisms involved in the development of occlusive arterial lesions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Single-cell transcriptional analysis resolved 44 global lung cell populations, with widespread early transcriptomic changes at 1 week affecting endothelial, stromal, and immune cell populations. In particular, two EC clusters were greatly expanded during PAH development and were identified as being disease specific: a relatively de-differentiated (dD) EC population that was enriched for Cd74 expression while exhibiting a loss of endothelial identity; and an activated arterial EC (aAEC) population that uniquely exhibited persistent differential gene expression throughout PAH development consistent with a growth regulated state. dDECs were primed to undergo endothelial\u2013mesenchymal transition as evidenced by reduced activity of master EC transcription factors, Erg and Fli1, and further supported by RNA velocity analysis showing vectors leading to fibroblast clusters. Of note, aAECs exhibited high expression of Tm4sf1, a gene implicated in cancer cell growth, that was also expressed by a smooth muscle (SM)-like pericyte cluster, and were highly localized to regions of arterial remodelling in both the rat model and PAH patients, contributing to intimal occlusive lesions and SM-like pericytes forming bands of medial muscularization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Together these findings implicate disease-specific vascular cells in PAH progression and suggest that TM4SF1 may be a novel therapeutic target for arterial remodelling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cvr\/cvaf146","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T11:29:37Z","timestamp":1756207777000},"page":"2095-2112","source":"Crossref","is-referenced-by-count":12,"title":["Mapping disease-specific vascular cell populations responsible for obliterative arterial remodelling during the development of pulmonary arterial hypertension"],"prefix":"10.1093","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8061-806X","authenticated-orcid":false,"given":"Nicholas D","family":"Cober","sequence":"first","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]},{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa , 501 Smyth Road, Box\/C.P. 511, Ottawa, ON, K1H 8L6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"McCourt","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Soares","family":"Godoy","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupu","family":"Deng","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Schlosser","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elmira Safaie","family":"Qamsari","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalil","family":"Azami","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Salehisiavashani","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7639-6724","authenticated-orcid":false,"given":"David P","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa , 501 Smyth Road, Box\/C.P. 511, Ottawa, ON, K1H 8L6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah-Eve","family":"Lemay","sequence":"additional","affiliation":[{"name":"Institut Universitaire de Cardiologie et de Pneumologie de Quebec, Research Center Laval University , Quebec City, QC ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Klouda","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Boston Children\u2019s Hospital, Harvard Medical School , Boston, MA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Yuan","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine, Boston Children\u2019s Hospital, Harvard Medical School , Boston, MA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Bonnet","sequence":"additional","affiliation":[{"name":"Institut Universitaire de Cardiologie et de Pneumologie de Quebec, Research Center Laval University , Quebec City, QC ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9113-8691","authenticated-orcid":false,"given":"Duncan J","family":"Stewart","sequence":"additional","affiliation":[{"name":"Sinclair Centre for Regenerative Medicine Program, Ottawa Hospital Research Institute , Ottawa, ON ,","place":["Canada"]},{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa , 501 Smyth Road, Box\/C.P. 511, Ottawa, ON, K1H 8L6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"2025102811285226400_cvaf146-B1","doi-asserted-by":"crossref","first-page":"1801913","DOI":"10.1183\/13993003.01913-2018","article-title":"Haemodynamic definitions and updated clinical classification of pulmonary hypertension","volume":"53","author":"Simonneau","year":"2019","journal-title":"Eur Respir J"},{"key":"2025102811285226400_cvaf146-B2","doi-asserted-by":"crossref","first-page":"2003957","DOI":"10.1183\/13993003.03957-2020","article-title":"Endothelial cells in the pathogenesis of pulmonary arterial hypertension","volume":"58","author":"Evans","year":"2021","journal-title":"Eur Respir J"},{"key":"2025102811285226400_cvaf146-B3","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1056\/NEJMoa2213558","article-title":"Phase 3 trial of sotatercept for treatment of pulmonary arterial hypertension","volume":"388","author":"Hoeper","year":"2023","journal-title":"N Engl J Med"},{"key":"2025102811285226400_cvaf146-B4","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1161\/HYPERTENSIONAHA.122.18261","article-title":"Evolving concepts in endothelial pathobiology of pulmonary arterial hypertension","volume":"79","author":"Cober","year":"2022","journal-title":"Hypertension"},{"key":"2025102811285226400_cvaf146-B5","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1161\/CIRCULATIONAHA.120.052318","article-title":"Integrated single-cell atlas of endothelial cells of the human lung","volume":"144","author":"Schupp","year":"2021","journal-title":"Circulation"},{"key":"2025102811285226400_cvaf146-B6","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1038\/s41586-018-0590-4","article-title":"Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris","volume":"562","author":"Tabula Muris Consortium; Overall coordination; Logistical coordination; Organ collection and processing; Library preparation and sequencing; Computational data analysis; Cell type annotation; Writing group; Supplemental text writing group; Principal investigators","year":"2018","journal-title":"Nature"},{"key":"2025102811285226400_cvaf146-B7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-021-21865-2","article-title":"Single cell transcriptomic analysis of murine lung development on hyperoxia-induced damage","volume":"12","author":"Hurskainen","year":"2021","journal-title":"Nat Commun"},{"key":"2025102811285226400_cvaf146-B8","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1038\/s41591-023-02327-2","article-title":"An integrated cell atlas of the lung in health and disease","volume":"29","author":"Sikkema","year":"2023","journal-title":"Nat Med"},{"key":"2025102811285226400_cvaf146-B9","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1164\/rccm.202006-2169OC","article-title":"Single-cell study of two rat models of pulmonary arterial hypertension reveals connections to human pathobiology and drug repositioning","volume":"203","author":"Hong","year":"2021","journal-title":"Am J Respir Crit Care Med"},{"key":"2025102811285226400_cvaf146-B10","doi-asserted-by":"crossref","first-page":"e142","DOI":"10.1093\/cvr\/cvad075","article-title":"Single-cell RNA-Seq profiling of mouse endothelial cells in response to pulmonary arterial hypertension","volume":"119","author":"Rodor","year":"2023","journal-title":"Cardiovasc Res"},{"key":"2025102811285226400_cvaf146-B11","first-page":"1","article-title":"Single-cell transcriptomic profile of human pulmonary artery endothelial cells in health and pulmonary arterial hypertension","volume":"11","author":"Asosingh","year":"2021","journal-title":"Sci Rep"},{"key":"2025102811285226400_cvaf146-B12","doi-asserted-by":"crossref","first-page":"e153471","DOI":"10.1172\/jci.insight.153471","article-title":"Single-cell transcriptomics reveals skewed cellular communication and phenotypic shift in pulmonary artery remodeling","volume":"7","author":"Crnkovic","year":"2022","journal-title":"JCI Insight"},{"key":"2025102811285226400_cvaf146-B13","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1038\/s41592-019-0433-8","article-title":"MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices","volume":"16","author":"McGinnis","year":"2019","journal-title":"Nat Methods"},{"key":"2025102811285226400_cvaf146-B14","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1096\/fj.00-0343com","article-title":"Inhibition of the VEGF receptor 2 combined with chronic hypoxia causes cell death-dependent pulmonary endothelial cell proliferation and severe pulmonary hypertension","volume":"15","author":"Taraseviciene-Stewart","year":"2001","journal-title":"FASEB J"},{"key":"2025102811285226400_cvaf146-B15","doi-asserted-by":"crossref","first-page":"3976","DOI":"10.1111\/bph.14472","article-title":"Efficacy of treprostinil in the SU5416-hypoxia model of severe pulmonary arterial hypertension: haemodynamic benefits are not associated with improvements in arterial remodelling","volume":"175","author":"Chaudhary","year":"2018","journal-title":"Br J Pharmacol"},{"key":"2025102811285226400_cvaf146-B16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2045894019883613","article-title":"Optimizing imaging of the rat pulmonary microvasculature by micro-computed tomography","volume":"9","author":"Deng","year":"2019","journal-title":"Pulm Circ"},{"key":"2025102811285226400_cvaf146-B17","doi-asserted-by":"crossref","first-page":"e80900","DOI":"10.7554\/eLife.80900","article-title":"Single-cell transcriptomic atlas of lung microvascular regeneration after targeted endothelial cell ablation","volume":"12","author":"Godoy","year":"2023","journal-title":"Elife"},{"key":"2025102811285226400_cvaf146-B18","doi-asserted-by":"crossref","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","article-title":"Integrated analysis of multimodal single-cell data","volume":"184","author":"Hao","year":"2021","journal-title":"Cell"},{"key":"2025102811285226400_cvaf146-B19","doi-asserted-by":"crossref","first-page":"979","DOI":"10.12688\/f1000research.73600.1","article-title":"Doublet identification in single-cell sequencing data using scDblFinder","volume":"10","author":"Germain","year":"2021","journal-title":"F1000Res"},{"key":"2025102811285226400_cvaf146-B20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.cels.2019.06.004","article-title":"SingleCellNet: a computational tool to classify single cell RNA-Seq data across platforms and across species","volume":"9","author":"Tan","year":"2019","journal-title":"Cell Syst"},{"key":"2025102811285226400_cvaf146-B21","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41587-020-0605-1","article-title":"Cell type prioritization in single-cell data","volume":"39","author":"Skinnider","year":"2021","journal-title":"Nat Biotechnol"},{"key":"2025102811285226400_cvaf146-B22","doi-asserted-by":"crossref","first-page":"6077","DOI":"10.1038\/s41467-020-19894-4","article-title":"Muscat detects subpopulation-specific state transitions from multi-sample multi-condition single-cell transcriptomics data","volume":"11","author":"Crowell","year":"2020","journal-title":"Nat Commun"},{"key":"2025102811285226400_cvaf146-B23","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.cels.2015.12.004","article-title":"The molecular signatures database hallmark gene set collection","volume":"1","author":"Liberzon","year":"2015","journal-title":"Cell Syst"},{"key":"2025102811285226400_cvaf146-B24","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2025102811285226400_cvaf146-B25","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1093\/bioinformatics\/btt703","article-title":"Causal analysis approaches in ingenuity pathway analysis","volume":"30","author":"Kr\u00e4mer","year":"2014","journal-title":"Bioinformatics"},{"key":"2025102811285226400_cvaf146-B26","doi-asserted-by":"crossref","DOI":"10.1093\/bioadv\/vbac016","article-title":"decoupleR: ensemble of computational methods to infer biological activities from omics data","volume":"2","author":"Badia-i-Mompel","year":"2022","journal-title":"Bioinform Adv"},{"key":"2025102811285226400_cvaf146-B27","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1101\/gr.240663.118","article-title":"Benchmark and integration of resources for the estimation of human transcription factor activities","volume":"29","author":"Garcia-Alonso","year":"2019","journal-title":"Genome Res"},{"key":"2025102811285226400_cvaf146-B28","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1038\/s41587-020-0591-3","article-title":"Generalizing RNA velocity to transient cell states through dynamical modeling","volume":"38","author":"Bergen","year":"2020","journal-title":"Nat Biotechnol"},{"key":"2025102811285226400_cvaf146-B29","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1038\/s41592-021-01346-6","article-title":"CellRank for directed single-cell fate mapping","volume":"19","author":"Lange","year":"2022","journal-title":"Nat Methods"},{"key":"2025102811285226400_cvaf146-B30","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1152\/ajplung.00217.2009","article-title":"Animal models of pulmonary arterial hypertension: the hope for etiological discovery and pharmacological cure","volume":"297","author":"Stenmark","year":"2009","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2025102811285226400_cvaf146-B31","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1016\/j.cell.2020.01.015","article-title":"Single-cell transcriptome atlas of murine endothelial cells","volume":"180","author":"Kalucka","year":"2020","journal-title":"Cell"},{"key":"2025102811285226400_cvaf146-B32","doi-asserted-by":"crossref","first-page":"1146399","DOI":"10.3389\/fcell.2023.1146399","article-title":"Cellular heterogeneity and stem cells of vascular endothelial cells in blood vessel formation and homeostasis: insights from single-cell RNA sequencing","volume":"11","author":"Wakabayashi","year":"2023","journal-title":"Front Cell Dev Biol"},{"key":"2025102811285226400_cvaf146-B33","doi-asserted-by":"crossref","first-page":"202","DOI":"10.3389\/fmolb.2020.00202","article-title":"Role of transmembrane 4 L six family 1 in the development and progression of cancer","volume":"7","author":"Fu","year":"2020","journal-title":"Front Mol Biosci"},{"key":"2025102811285226400_cvaf146-B34","doi-asserted-by":"crossref","first-page":"111978","DOI":"10.1016\/j.celrep.2022.111978","article-title":"Absolute scaling of single-cell transcriptomes identifies pervasive hypertranscription in adult stem and progenitor cells","volume":"42","author":"Kim","year":"2023","journal-title":"Cell Rep"},{"key":"2025102811285226400_cvaf146-B35","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1161\/CIRCULATIONAHA.114.008777","article-title":"Proteomic analysis implicates translationally controlled tumor protein as a novel mediator of occlusive vascular remodeling in pulmonary arterial hypertension","volume":"129","author":"Lavoie","year":"2014","journal-title":"Circulation"},{"key":"2025102811285226400_cvaf146-B36","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1165\/rcmb.2017-0129OC","article-title":"A potential role for exosomal translationally controlled tumor protein export in vascular remodeling in pulmonary arterial hypertension","volume":"59","author":"Ferrer","year":"2018","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025102811285226400_cvaf146-B37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2045894020908782","article-title":"Transcriptional profiling of lung cell populations in idiopathic pulmonary arterial hypertension","volume":"10","author":"Saygin","year":"2020","journal-title":"Pulm Circ"},{"key":"2025102811285226400_cvaf146-B38","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1038\/s41467-020-17740-1","article-title":"Single-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination","volume":"11","author":"Muhl","year":"2020","journal-title":"Nat Commun"},{"key":"2025102811285226400_cvaf146-B39","doi-asserted-by":"crossref","first-page":"L86","DOI":"10.1152\/ajplung.00356.2002","article-title":"Targeted blocking of gene expression for CGRP receptors elevates pulmonary artery pressure in hypoxic rats","volume":"285","author":"Qing","year":"2003","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2025102811285226400_cvaf146-B40","doi-asserted-by":"crossref","first-page":"L135","DOI":"10.1152\/ajplung.00084.2019","article-title":"TRPV4 channel mediates adventitial fibroblast activation and adventitial remodeling in pulmonary hypertension","volume":"318","author":"Cussac","year":"2020","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2025102811285226400_cvaf146-B41","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1161\/ATVBAHA.113.302062","article-title":"Soluble JAGGED1 inhibits pulmonary hypertension by attenuating notch signaling","volume":"33","author":"Xiao","year":"2013","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2025102811285226400_cvaf146-B42","doi-asserted-by":"crossref","first-page":"658","DOI":"10.3390\/life12050658","article-title":"The platelet-derived growth factor pathway in pulmonary arterial hypertension: still an interesting target?","volume":"12","author":"Solinc","year":"2022","journal-title":"Life"},{"key":"2025102811285226400_cvaf146-B43","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1161\/CIRCRESAHA.119.315054","article-title":"Role of the aryl hydrocarbon receptor\/ARNT\/cytochrome P450 system in pulmonary vascular diseases","volume":"125","author":"Kwapiszewska","year":"2019","journal-title":"Circ Res"},{"key":"2025102811285226400_cvaf146-B44","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1161\/CIRCULATIONAHA.116.022479","article-title":"Endothelial-to-mesenchymal transition","volume":"133","author":"Stenmark","year":"2016","journal-title":"Circulation"},{"key":"2025102811285226400_cvaf146-B45","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/s41580-020-0237-9","article-title":"Guidelines and definitions for research on epithelial\u2013mesenchymal transition","volume":"21","author":"Yang","year":"2020","journal-title":"Nat Rev Mol Cell Biol"},{"key":"2025102811285226400_cvaf146-B46","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1161\/CIRCRESAHA.118.313788","article-title":"The transcription factor ERG regulates super-enhancers associated with an endothelial-specific gene expression program","volume":"124","author":"Kalna","year":"2019","journal-title":"Circ Res"},{"key":"2025102811285226400_cvaf146-B47","doi-asserted-by":"crossref","first-page":"e1007826","DOI":"10.1371\/journal.pgen.1007826","article-title":"Downregulation of ERG and FLI1 expression in endothelial cells triggers endothelial-to-mesenchymal transition","volume":"14","author":"Nagai","year":"2018","journal-title":"PLoS Genet"},{"key":"2025102811285226400_cvaf146-B48","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1186\/s12885-020-07107-6","article-title":"NR2F2 plays a major role in insulin-induced epithelial-mesenchymal transition in breast cancer cells","volume":"20","author":"Xia","year":"2020","journal-title":"BMC Cancer"},{"key":"2025102811285226400_cvaf146-B49","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1161\/CIRCRESAHA.113.301141","article-title":"Inflammation and immunity in the pathogenesis of pulmonary arterial hypertension","volume":"115","author":"Rabinovitch","year":"2014","journal-title":"Circ Res"},{"key":"2025102811285226400_cvaf146-B50","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1093\/cvr\/cvw070","article-title":"Haemodynamic unloading reverses occlusive vascular lesions in severe pulmonary hypertension","volume":"111","author":"Abe","year":"2016","journal-title":"Cardiovasc Res"},{"key":"2025102811285226400_cvaf146-B51","doi-asserted-by":"crossref","first-page":"1410","DOI":"10.1164\/rccm.201506-1231OC","article-title":"Delayed microvascular shear adaptation in pulmonary arterial hypertension. Role of platelet endothelial cell adhesion molecule-1 cleavage","volume":"193","author":"Szulcek","year":"2016","journal-title":"Am J Respir Crit Care Med"},{"key":"2025102811285226400_cvaf146-B52","doi-asserted-by":"publisher","author":"Deng","DOI":"10.1101\/2023.10.16.562339"},{"key":"2025102811285226400_cvaf146-B53","doi-asserted-by":"crossref","first-page":"L548","DOI":"10.1152\/ajplung.00428.2006","article-title":"Hyperproliferative apoptosis-resistant endothelial cells in idiopathic pulmonary arterial hypertension","volume":"293","author":"Masri","year":"2007","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2025102811285226400_cvaf146-B54","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1165\/rcmb.2019-0401OC","article-title":"Mural cell SDF1 signaling is associated with the pathogenesis of pulmonary arterial hypertension","volume":"62","author":"Yuan","year":"2020","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2025102811285226400_cvaf146-B55","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1161\/ATVBAHA.119.313715","article-title":"Lineage tracing reveals the dynamic contribution of pericytes to the blood vessel remodeling in pulmonary hypertension","volume":"40","author":"Bordenave","year":"2020","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"2025102811285226400_cvaf146-B56","doi-asserted-by":"crossref","first-page":"2227","DOI":"10.1002\/j.2040-4603.2021.tb00178.x","article-title":"Lung pericytes in pulmonary vascular physiology and pathophysiology","volume":"11","author":"Yuan","year":"2021","journal-title":"Compr Physiol"},{"key":"2025102811285226400_cvaf146-B57","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.imlet.2023.01.011","article-title":"Construction of CAR-T cells targeting TM4SF1 and its anti-tumor capacity in ovarian cancer","volume":"255","author":"Shen","year":"2023","journal-title":"Immunol Lett"},{"key":"2025102811285226400_cvaf146-B58","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/s41392-022-01177-7","article-title":"Targeting TM4SF1 exhibits therapeutic potential via inhibition of cancer stem cells","volume":"7","author":"Chen","year":"2022","journal-title":"Signal Transduct Target Ther"},{"key":"2025102811285226400_cvaf146-B59","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1007\/s10456-014-9437-2","article-title":"TM4SF1: a new vascular therapeutic target in cancer","volume":"17","author":"Lin","year":"2014","journal-title":"Angiogenesis"},{"key":"2025102811285226400_cvaf146-B60","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1164\/rccm.201402-0322OC","article-title":"Proinflammatory signature of the dysfunctional endothelium in pulmonary hypertension. Role of the macrophage migration inhibitory factor\/CD74 Complex","volume":"192","author":"Le Hiress","year":"2015","journal-title":"Am J Respir Crit Care Med"},{"key":"2025102811285226400_cvaf146-B61","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1089\/ars.2020.8169","article-title":"Endothelial-to-mesenchymal transition in pulmonary arterial hypertension","volume":"34","author":"Gorelova","year":"2021","journal-title":"Antioxid Redox Signal"},{"key":"2025102811285226400_cvaf146-B62","doi-asserted-by":"crossref","first-page":"10010","DOI":"10.1093\/nar\/gkz805","article-title":"Signatures of cell death and proliferation in perturbation transcriptomics data\u2014from confounding factor to effective prediction","volume":"47","author":"Szalai","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2025102811285226400_cvaf146-B63","doi-asserted-by":"crossref","first-page":"108077","DOI":"10.1016\/j.celrep.2020.108077","article-title":"Single-cell transcriptome analysis of colon cancer cell response to 5-fluorouracil-induced DNA damage","volume":"32","author":"Park","year":"2020","journal-title":"Cell Rep"},{"key":"2025102811285226400_cvaf146-B64","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.lfs.2018.05.054","article-title":"CRIP1 promotes cell migration, invasion and epithelial-mesenchymal transition of cervical cancer by activating the Wnt\/\u03b2-catenin signaling pathway","volume":"207","author":"Zhang","year":"2018","journal-title":"Life Sci"}],"container-title":["Cardiovascular Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/cardiovascres\/advance-article-pdf\/doi\/10.1093\/cvr\/cvaf146\/64150386\/cvaf146.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article-pdf\/121\/13\/2095\/64150386\/cvaf146.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article-pdf\/121\/13\/2095\/64150386\/cvaf146.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T15:29:04Z","timestamp":1761665344000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cardiovascres\/article\/121\/13\/2095\/8242845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,28]]},"references-count":64,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2025,8,28]]},"published-print":{"date-parts":[[2025,10,28]]}},"URL":"https:\/\/doi.org\/10.1093\/cvr\/cvaf146","relation":{},"ISSN":["0008-6363","1755-3245"],"issn-type":[{"value":"0008-6363","type":"print"},{"value":"1755-3245","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,10]]},"published":{"date-parts":[[2025,8,28]]}}}