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It revealed original vistas into the mechanisms of CAVD and novel putative therapeutic targets. This review provides a narrative state-of-the-art overview of scRNA-seq applications in CAVD research, summarizing key findings on valvular cell populations, disease-associated phenotypic transitions, and molecular pathways driving pathological remodeling. Additionally, the translational potential of single-cell technologies in identifying novel therapeutic targets is discussed, as well as the current challenges and future directions in this rapidly evolving field.<\/jats:p>","DOI":"10.1093\/cvr\/cvaf255","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T12:58:12Z","timestamp":1763470692000},"page":"2838-2859","source":"Crossref","is-referenced-by-count":7,"title":["Advances in single-cell transcriptomics: unraveling the pathogenesis of calcific aortic valve disease"],"prefix":"10.1093","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-6096","authenticated-orcid":false,"given":"Zihan","family":"Qin","sequence":"first","affiliation":[{"name":"Translational Cardiology, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet , Visionsgatan 18, Stockholm 17176 ,","place":["Sweden"]},{"name":"Department of Cardiology, Heart and Vascular Center, Karolinska University Hospital , Eugeniav\u00e4gen 3, Stockholm 17176 ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0853-5141","authenticated-orcid":false,"given":"Magnus","family":"B\u00e4ck","sequence":"additional","affiliation":[{"name":"Translational Cardiology, Department of Medicine Solna, Center for Molecular Medicine, Karolinska Institutet , Visionsgatan 18, Stockholm 17176 ,","place":["Sweden"]},{"name":"Department of Cardiology, Heart and Vascular Center, Karolinska University Hospital , Eugeniav\u00e4gen 3, Stockholm 17176 ,","place":["Sweden"]},{"name":"Department of Chronic and Acute Cardiovascular Disease, Universit\u00e9 de Lorraine, Inserm, DCAC , 9 Avenue de la For\u00eat de Haye, Nancy F-54000 ,","place":["France"]},{"name":"Department of Research and Innovation, Centre Hospitalier R\u00e9gional Universitaire de Nancy , 29 Av. du Mar\u00e9chal de Lattre de Tassigny, Vandoeuvre-l\u00e8s-Nancy F-54000 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