{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:38:32Z","timestamp":1793093912078,"version":"4.5.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101098372"],"award-info":[{"award-number":["101098372"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["101070953"],"award-info":[{"award-number":["101070953"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"NWO","doi-asserted-by":"publisher","award":["NWO\/ENW-XL 2019.029"],"award-info":[{"award-number":["NWO\/ENW-XL 2019.029"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Engineered cardiac tissue models for in vitro physiological studies often fail to replicate the pump function of the heart. Despite promising advancements, the use of engineered cardiac chambers is often hindered by complex fabrication processes and invasive characterization techniques. Here, we engineered a chamber-like human cardiac model with pumping function, referred to as a \u2018mini-heart\u2019, by employing a novel sacrificial moulding approach within a customized bioreactor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>Human pluripotent stem cell-derived cardiomyocytes and human cardiac fibroblasts were embedded in a fibrin mix and casted around two gelatin bodies within a customized bioreactor. After fibrin polymerization, thermal degradation of gelatin was induced to obtain a single-inlet cardiac chamber coupled to a glass capillary inlet.<\/jats:p>\n                    <jats:p>The mini-heart's pumping capability was confirmed through optical recording of fluid displacement at the engineered tissue inlet, enabling the non-invasive acquisition of haemodynamic parameters such as stroke volume, stroke work, ejection fraction, and developed pressure. Morphological analysis of the engineered tissues revealed organized sarcomeres and extracellular matrix self-determination, highlighting the advantage of our degradable mould technology. Additionally, we measured calcium transients during both spontaneous and electrically paced beating and observed a positive inotropic response to the \u03b2-adrenergic agonist drug isoproterenol.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We present a biomimetic, chamber-like human cardiac model with intrinsic pumping function that enables non-invasive functional assessment of cardiac haemodynamics in vitro.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/cvr\/cvag210","type":"journal-article","created":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:21:55Z","timestamp":1789903315000},"source":"Crossref","is-referenced-by-count":0,"title":["A human-engineered mini-heart platform for mimicking ventricular pump function"],"prefix":"10.1093","author":[{"given":"Marcelo C","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Applied Stem Cell Technologies, Department of BioEngineering Technologies, Cardiovascular Health Technology Centre, TechMed Centre, University of Twente , P.O. Box 217, 7500 AE Enschede ,","place":["the Netherlands"]},{"name":"River BioMedics B.V. , 7522 NB Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7827-9367","authenticated-orcid":false,"given":"Mariel","family":"Cano-Jorge","sequence":"additional","affiliation":[{"name":"Applied Stem Cell Technologies, Department of BioEngineering Technologies, Cardiovascular Health Technology Centre, TechMed Centre, University of Twente , P.O. Box 217, 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"ten Den","sequence":"additional","affiliation":[{"name":"Applied Stem Cell Technologies, Department of BioEngineering Technologies, Cardiovascular Health Technology Centre, TechMed Centre, University of Twente , P.O. Box 217, 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1891-582X","authenticated-orcid":false,"given":"Danique","family":"Snippert","sequence":"additional","affiliation":[{"name":"Applied Stem Cell Technologies, Department of BioEngineering Technologies, Cardiovascular Health Technology Centre, TechMed Centre, University of Twente , P.O. Box 217, 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Karperien","sequence":"additional","affiliation":[{"name":"Developmental BioEngineering, Department of BioEngineering Technologies, TechMed Centre, University of Twente , 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Kamperman","sequence":"additional","affiliation":[{"name":"IamFluidics B.V. , High Tech Factory, 7522 NM Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"Lajoinie","sequence":"additional","affiliation":[{"name":"Physics of Fluids Group, Max Planck Center Twente for Complex Fluid Dynamics, TechMed Centre, University of Twente , 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Versluis","sequence":"additional","affiliation":[{"name":"Physics of Fluids Group, Max Planck Center Twente for Complex Fluid Dynamics, TechMed Centre, University of Twente , 7500 AE Enschede ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4312-9296","authenticated-orcid":false,"given":"Robert","family":"Passier","sequence":"additional","affiliation":[{"name":"Applied Stem Cell Technologies, Department of BioEngineering Technologies, Cardiovascular Health Technology Centre, TechMed Centre, University of Twente , P.O. Box 217, 7500 AE Enschede ,","place":["the Netherlands"]},{"name":"Department of Anatomy and Embryology, Leiden University Medical Centre , P.O. Box 9600, 2300 RC Leiden ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"2026102705313598500_cvag210-B1","doi-asserted-by":"crossref","first-page":"E93","DOI":"10.1161\/CIR.0000000000001123","article-title":"Heart disease and stroke statistics - 2023 update: a report from the American Heart Association","volume":"147","author":"Tsao","year":"2023","journal-title":"Circulation"},{"key":"2026102705313598500_cvag210-B2","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1016\/j.jacc.2015.03.016","article-title":"Cardiovascular drug development is it dead or just hibernating?","volume":"65","author":"Fordyce","year":"2015","journal-title":"J Am Coll Cardiol"},{"key":"2026102705313598500_cvag210-B3","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1093\/cvr\/cvab075","article-title":"Alternative strategies in cardiac preclinical research and new clinical trial formats","volume":"118","author":"Kreutzer","year":"2022","journal-title":"Cardiovasc Res"},{"key":"2026102705313598500_cvag210-B4","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1186\/s12967-018-1678-1","article-title":"Is it possible to overcome issues of external validity in preclinical animal research? 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