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These archaea play crucial roles in global carbon cycling and anaerobic environments, yet scattered data and unculturable strains limit systematic studies and applications. To address this, we created MethArDB (Methanogenic Archaeal Genome Database), a specialized database for methanogenic archaea, compiling 3919 genomes, 87 host-associated plasmids, and 42 phages, with standardized quality classifications (complete, scaffold, draft), protein sequences, and metadata on geography, habitats, metabolism, and inheritable elements. Integrated MethArCT (Methanogenic Archaeal Culturomics Toolkit) employs a dual-threshold orthologous\/paralogous protein analysis to evaluate metabolic pathway completeness, predicting cultivation parameters and suggesting candidate cultivation strategies, including potential medium formulations and conditions, to support strain isolation. Overall, MethArDB and MethArCT form an integrated platform combining genomics and culturomics to facilitate methanogenic archaea research.<\/jats:p>\n                  <jats:p>Database URL: \u00a0http:\/\/methardb.cn<\/jats:p>","DOI":"10.1093\/database\/baag055","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:30:29Z","timestamp":1788521429000},"source":"Crossref","is-referenced-by-count":0,"title":["An integrated culturomic and genomic database and analysis platform for methanogenic archaea"],"prefix":"10.1093","volume":"2026","author":[{"given":"Jiuyu","family":"Chen","sequence":"first","affiliation":[{"name":"Bioenergy Research Institute, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University , Nanjing, Jiangsu 211816 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Ren","sequence":"additional","affiliation":[{"name":"Bioenergy Research Institute, College of 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