{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T10:19:12Z","timestamp":1783937952178,"version":"3.55.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Increasing genome data are coming out. Genome size estimation plays an essential role in guiding genome assembly. Several months ago, other researchers were the first to publish a draft genome of the red gromwell (i.e. Lithospermum erythrorhizon). However, we considered that the genome size they estimated and assembled was incorrect. This study meticulously estimated the L. erythrorhizon genome size to should be \u223c708.74\u2009Mb and further provided a reliable genome version (size \u2248 693.34\u2009Mb; contigN50 length \u2248 238.08 Kb) to support our objection. Furthermore, according to our genome, we identified a gene family of the alkannin\/shikonin O-acyltransferases (i.e. AAT\/SAT) that catalysed enantiomer-specific acylations in the alkannin\/shikonin biosynthesis (a characteristic metabolic pathway in L. erythrorhizon\u2019s roots) and further explored its evolutionary process. The results indicated that the existing AAT\/SAT were not generated from only one round of gene duplication but three rounds; after different rounds of gene duplication, the existing AAT\/SAT and their recent ancestors were under positive selection at different amino acid sites. These suggested that a combined power from gene duplication plus positive selection plausibly propelled AAT\/SAT\u2019s functional differentiation in evolution.<\/jats:p>","DOI":"10.1093\/dnares\/dsab015","type":"journal-article","created":{"date-parts":[[2021,8,19]],"date-time":"2021-08-19T19:25:00Z","timestamp":1629401100000},"source":"Crossref","is-referenced-by-count":11,"title":["Exploring the evolutionary process of alkannin\/shikonin <i>O<\/i>-acyltransferases by a reliable <i>Lithospermum erythrorhizon<\/i> genome"],"prefix":"10.1093","volume":"28","author":[{"given":"Chengyi","family":"Tang","sequence":"first","affiliation":[{"name":"School of the Environment, Nanjing University , Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"2022061714311698100_dsab015-B1","author":"The Plant List","year":"2013"},{"key":"2022061714311698100_dsab015-B2","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1111\/nph.13191","article-title":"The chromosome counts database (CCDB) \u2013 a community resource of plant chromosome numbers","volume":"206","author":"Rice","year":"2015","journal-title":"New Phytol"},{"key":"2022061714311698100_dsab015-B3","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.ympev.2009.05.013","article-title":"Marbleseeds are gromwells \u2013 systematics and evolution of Lithospermum and allies (Boraginaceae tribe Lithospermeae) based on molecular and morphological data","volume":"52","author":"Weigend","year":"2009","journal-title":"Mol. 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