{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T04:21:37Z","timestamp":1765254097377,"version":"3.41.2"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Hacettepe University Scientific Research Council","award":["THD-2019-17885"],"award-info":[{"award-number":["THD-2019-17885"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>MicroRNAs (miRNAs) are noncoding RNAs that play an important role in the regulation of inflammation and have not been evaluated in exhaled breath condensates (EBC) of patients with esophageal atresia and tracheoesophageal fistula (EA\u2013TEF). It is aimed to evaluate the levels of miRNA-21 and miRNA-24 in EBC of patients with EA\u2013TEF.<\/jats:p>\n               <jats:p>Patients who received surgery for EA\u2013TEF (EA) were assessed for age, sex, types of anomaly, surgical treatments, and respiratory problems. A 500\u20131000 mL of EBC was obtained from each participant with EcoScreen. The levels of miRNA-21 and miRNA-24 in the EBC were analyzed by real-time polymerase chain reaction and compared between the EA group and the control group consisting of healthy children with no history of respiratory problems (n\u00a0=\u200917). The levels of miRNAs in relation to respiratory problems and gastroesophageal reflux (GER) were also assessed. A total of 19 patients were enrolled in the EA group with a mean age of 7.8 \u00b1 3.2 years and a male-to-female ratio of 10:9 EA cases had significantly lower levels of miRNA-21 (P\u00a0&amp;lt;\u20090.05) compared to that in control group. The miRNA-24 levels did not differ between groups (P\u00a0&amp;gt;\u20090.05). EA patients with positive pH testing for GER (n\u00a0=\u20096) and fundoplication (n\u00a0=\u20096) had higher levels of miRNA-21 than those with normal pH testing and without fundoplication, respectively (n\u00a0=\u200913, P\u00a0&amp;lt;\u20090.05). The levels of miRNA-21 and miRNA-24 did not differ between patients with and without proton pump inhibitor treatment (P\u00a0&amp;gt;\u20090.05).<\/jats:p>\n               <jats:p>The lower levels of miRNA-21 in the EBC of EA patients suggest a hyperreactive airway problem, which may be associated with GER and its surgical treatment.<\/jats:p>","DOI":"10.1093\/dote\/doaa082","type":"journal-article","created":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T05:12:19Z","timestamp":1598937139000},"source":"Crossref","is-referenced-by-count":7,"title":["MicroRNA Levels in Exhaled Breath Condensate of Patients with Esophageal Atresia"],"prefix":"10.1093","volume":"34","author":[{"given":"T","family":"Soyer","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Birben","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6 B","family":"T\u00fcrer","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kahveci","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G D","family":"Tu\u011fcu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6 U","family":"Soyer","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Yal\u00e7\u0131n","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Do\u011fru","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U","family":"\u00d6z\u00e7elik","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Kiper","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pulmonology, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B E","family":"\u015eekerel","sequence":"additional","affiliation":[{"name":"Department of Pediatric Allergy, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F C","family":"Tanyel","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery, Hacettepe University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2021031106042616700_ref1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/B978-0-323-07255-7.00069-6","volume-title":"Pediatric Surgery","author":"Harmon","year":"2012"},{"key":"2021031106042616700_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2019.06.007","article-title":"The relationship between oxidative stress markers in exhaled breath condensate and respiratory problems in patients with repaired esophageal atresia","author":"Soyer","year":"2019","journal-title":"J Pediatr Surg"},{"key":"2021031106042616700_ref3","first-page":"77","article-title":"Respiratory problems in children with esophageal atresia and tracheaesophageal fistula","volume":"43","author":"Porcaro","year":"2017","journal-title":"J Pediatr"},{"key":"2021031106042616700_ref4","first-page":"527","article-title":"Etiology and management of respiratory complications after repair of esophageal atresia with tracheaesophageal fistula","volume":"112","author":"Delius","year":"1992","journal-title":"Surgery"},{"key":"2021031106042616700_ref5","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1378\/chest.126.3.915","article-title":"Long-term complications of congenital esophageal atresia and\/or tracheoesophageal fistula","volume":"126","author":"Kovesi","year":"2004","journal-title":"Chest"},{"key":"2021031106042616700_ref6","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1046\/j.0905-6157.2003.00091.x","article-title":"Exhaled breath condensate in children: pearls and pitfalls","volume":"15","author":"Rosias","year":"2004","journal-title":"Pediatr Allergy Immunol"},{"key":"2021031106042616700_ref7","first-page":"17232","article-title":"Clinical significance of miRNA-21 in exhaled breath condensate of non-small-cell cancer","volume":"9","author":"Chen","year":"2016","journal-title":"Int Clin Exp Med"},{"key":"2021031106042616700_ref8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aca.2013.07.049","article-title":"Exhaled breath condensate: determination of non-volatile compounds and their potential for clinical diagnosis and monitoring. A review","volume":"805","author":"Kuban","year":"2013","journal-title":"Anal Chim Acta"},{"key":"2021031106042616700_ref9","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1111\/j.1398-9995.2006.01064.x","article-title":"Comparison of two methods for exhaled breath condensate collection","volume":"61","author":"Soyer","year":"2006","journal-title":"Allergy"},{"key":"2021031106042616700_ref10","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1111\/j.1398-9995.2008.01766.x","article-title":"Oxidative stress and its determinants in the airways of children with asthma","volume":"63","author":"Dut","year":"2008","journal-title":"Allergy"},{"key":"2021031106042616700_ref11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jaci.2013.03.006","article-title":"Differential expression of microRNA\u2019s in exhaled breath condensate of patients with asthma, patients with chronic obstructive pulmonary disease and healthy adults","volume":"132","author":"Pinkerton","year":"2013","journal-title":"J Allergy Clin Immunol"},{"key":"2021031106042616700_ref12","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1084\/jem.20100035","article-title":"miR-21 mediates fibrogenic activation of pulmonary fibroblasts and lung fibrosis","volume":"207","author":"Liu","year":"2010","journal-title":"J Exp Med"},{"key":"2021031106042616700_ref13","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1002\/ppul.22739","article-title":"MicroRNAs and lung development","volume":"48","author":"Khoshgoo","year":"2013","journal-title":"Pediatr Pulmonol"},{"key":"2021031106042616700_ref14","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/ppul.24517","article-title":"Pulmonary function in children and adolescents after esophageal atresia repair","volume":"55","author":"Donoso","year":"2020","journal-title":"Pediatr Pulmonol"},{"key":"2021031106042616700_ref15","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1055\/s-0039-1693729","article-title":"Clinical factors affecting condition-specific quality-of-life domains in pediatric patients after repair of esophageal atresia: the Swedish-German EA-QOL study","volume":"30","author":"Dellenmark-Blom","year":"2020","journal-title":"Eur J Pediatr Surg"},{"key":"2021031106042616700_ref16","first-page":"62","article-title":"Aspiration risk and respiratory complications in patients with esophageal atresia","volume":"3","author":"Kovesi","year":"2017","journal-title":"Front Pediatr"},{"key":"2021031106042616700_ref17","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1016\/j.jaci.2016.02.018","article-title":"Asthma inflammatory phenotypes show differential microRNA expression in sputum","volume":"137","author":"Maes","year":"2016","journal-title":"J Allergy Clin Immunol"},{"key":"2021031106042616700_ref18","doi-asserted-by":"crossref","first-page":"e0224983","DOI":"10.1371\/journal.pone.0224983","article-title":"Development and validation of exhaled breath condensate microRNAs to identify and endotype asthma in children","volume":"14","author":"Mendes","year":"2019 ( 8)","journal-title":"PLoS One"},{"key":"2021031106042616700_ref19","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1097\/MPG.0000000000001401","article-title":"ESPGHAN-NASPGHAN guidelines for the evaluation and treatment of gastrointestinal and nutritional complications in children with esophageal atresia-tracheaoesophageal fistula","volume":"63","author":"Krishnan","year":"2016","journal-title":"J Pediatr Gastroenterol Nutr"}],"container-title":["Diseases of the Esophagus"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/dote\/article-pdf\/34\/3\/doaa082\/36512896\/doaa082.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/dote\/article-pdf\/34\/3\/doaa082\/36512896\/doaa082.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T06:35:27Z","timestamp":1615444527000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/dote\/article\/doi\/10.1093\/dote\/doaa082\/5899730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,1]]},"references-count":19,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9,1]]},"published-print":{"date-parts":[[2021,3,8]]}},"URL":"https:\/\/doi.org\/10.1093\/dote\/doaa082","relation":{},"ISSN":["1120-8694","1442-2050"],"issn-type":[{"type":"print","value":"1120-8694"},{"type":"electronic","value":"1442-2050"}],"subject":[],"published-other":{"date-parts":[[2021,3]]},"published":{"date-parts":[[2020,9,1]]},"article-number":"doaa082"}}