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This study aimed to evaluate <jats:italic>in vitro<\/jats:italic> anti\u2010leukemic activity of dichloromethane extract\/fraction number 7 (DCM\/F7) from <jats:italic>T. flagelliforme<\/jats:italic> tuber on human T4 lymphoblastoid (CEMss) cell line. The DCM extract of tuber has been fractionated by column chromatography. The obtained fractions were evaluated for its cytotoxicity toward CEMss cells as well as human primary blood lymphocytes (PBLs). Assessment of apoptosis produced by the most active fraction was evaluated by various microscopic techniques and further confirmation of apoptosis was done by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Phytochemical screening was done by gas chromatography\u2010mass spectrometry (GC\u2010MS). The results shows that 7 out of 12 fractions showed significant cytotoxicity against the selected cell line CEMss, in which fractions DCM\/F7, DCM\/F11 and DCM\/F12 showed exceptional activity with 3, 5 and 6.2\u2009\u03bcg\u2009ml<jats:sup>\u22121<\/jats:sup>, respectively. Further studies in the non\u2010cancerous PBL exhibited significant selectivity of DCM\/F7 compared to other fractions. Cytological observations showed chromatin condensation, cell shrinkage, abnormalities of cristae, membrane blebbing, cytoplasmic extrusions and formation of apoptotic bodies as confirmed collectively by double\u2010staining of acridine orange (AO)\/propidium iodide (PI), SEM and TEM. In addition, DCM\/F7 has increased the cellular DNA breaks on treated cells. GC\u2010MS revealed that DCM\/F7 contains linoleic acid, hexadecanoic acid and 9\u2010hexadecanoic acid. The present results indicate that <jats:italic>T. flagelliforme<\/jats:italic> possess a valuable anti\u2010leukemic effect and was able to produce distinctive morphological features of cell death that corresponds to apoptosis.<\/jats:p>","DOI":"10.1093\/ecam\/neq010","type":"journal-article","created":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T01:47:04Z","timestamp":1266889624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["<i>In Vitro<\/i> Ultramorphological Assessment of Apoptosis on CEMss Induced by Linoleic Acid\u2010Rich Fraction from <i>Typhonium flagelliforme<\/i> Tuber"],"prefix":"10.1155","volume":"2011","author":[{"given":"Syam","family":"Mohan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad","family":"Bustamam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddig","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel S.","family":"Al-Zubairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Aspollah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasedee","family":"Abdullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal Mohamed","family":"Elhassan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,2]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1095\u20108339.2003.00139.x"},{"key":"e_1_2_6_2_2","first-page":"65","volume-title":"A Revised Hand Book to the Flora of Ceylon","author":"Dassanayake M. 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