{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:33Z","timestamp":1789171233201,"version":"build-2803163510"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T00:00:00Z","timestamp":1661385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870383"],"award-info":[{"award-number":["81870383"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2017YFC1308800"],"award-info":[{"award-number":["2017YFC1308800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Aims<\/jats:title><jats:p>Stem cell therapy is a promising cell-based treatment modality for inflammatory bowel diseases [IBD], but its application is limited by the nature of cell heterogeneity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Single-cell RNA-sequencing was performed on the adipose-derived stem cells [ADSCs]. The in vitro immunomodulatory effect of ADSCs was evaluated by co-culturing with human CD4+ T cells or macrophages. The in vivo therapeutic value of ADSCs was assessed using a murine colitis model induced by dextran sulphate sodium [DSS] or 2,4,6-trinitrobenzene sulphonic acid [TNBS].<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD200+ ADSCs were identified as a novel subpopulation of ADSCs, based on gene ontology analysis of immunoregulatory functions. The immunoregulatory functions of these cells were further confirmed by co-culturing with CD4+ T cells or macrophages. Administration of CD200+ ADSCs effectively reduced intestinal inflammation in IBD mice models. Furthermore, we found CD200+ ADSCs-derived GAS6 exerted protective effects on experimental colitis by promoting macrophage M2 polarization via the Mer\/PI3K\/Akt\/GSK3\u03b2 signalling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study uncovered the heterogeneity in ADSCs, in which CD200+ ADSCs presents as an alternative to conventional treatment of IBD.<\/jats:p><\/jats:sec>","DOI":"10.1093\/ecco-jcc\/jjac123","type":"journal-article","created":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T15:39:09Z","timestamp":1661441949000},"page":"289-301","source":"Crossref","is-referenced-by-count":19,"title":["GAS6 From CD200+ Adipose-Derived Stem Cells Mitigates Colonic Inflammation in a Macrophage-Dependent Manner"],"prefix":"10.1093","volume":"17","author":[{"given":"Zhen-Xing","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou, Guangdong , China"},{"name":"Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou, Guangdong , China"},{"name":"Bioland Laboratory, Guangzhou Regenerative Medicine and Health Guangdong Laboratory , Guangzhou , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-Shan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou, Guangdong , China"},{"name":"Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou, Guangdong , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University , Guangzhou, Guangdong , 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