{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:50:37Z","timestamp":1753876237087,"version":"3.41.2"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"crossref","award":["LY21H020007"],"award-info":[{"award-number":["LY21H020007"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100016114","name":"Zhejiang Medical Association","doi-asserted-by":"crossref","award":["ZYC-A117"],"award-info":[{"award-number":["ZYC-A117"]}],"id":[{"id":"10.13039\/501100016114","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Primary effusion lymphoma (PEL) is an uncommon subtype of non-Hodgkin lymphoma (NHL) that usually involves the pleura, pericardium, and peritoneum without an obvious tumour mass, with multiple plasma effusions as its main clinical feature. We report a case of a massive pericardial effusion in an elderly male with a final diagnosis of PEL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Case summary<\/jats:title>\n                  <jats:p>A 70-year-old male patient was admitted to hospital with symptoms of chest tightness, shortness of breath, fatigue, loss of appetite, and cough with phlegm after a pericardial effusion had been found for 5 months. The next-generation sequencing of pericardial effusion found human herpesvirus type 8 (HHV-8) infection, and further cytomorphological and immunohistochemical examination were done. According to the patient's HHV-8 infection, the pathological features of heterogeneous B cells with plasmablastic differentiation and the immunohistochemical characteristics of PEL, the final diagnosis was made as human immunodeficiency virus-negative PEL.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Discussion<\/jats:title>\n                  <jats:p>The diversity and non-specificity of PEL symptoms, as well as its rarity, make it difficult to diagnose. In this case, we used the next-generation sequencing technology to screen the pathogen of the patient's pericardial effusion and carried out morphological and immunohistochemical examination of the cells in the pericardial effusion, which provided a clinically operable diagnosis for an uncommon disease, enabling us to make a clear diagnosis faster and start treatment in time.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ehjcr\/ytac239","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T18:49:06Z","timestamp":1655837346000},"source":"Crossref","is-referenced-by-count":0,"title":["A case report-application of pericardial effusion cytology and next-generation sequencing technology: quick and secure diagnosis of primary effusion lymphoma"],"prefix":"10.1093","volume":"6","author":[{"given":"Jin Lei","family":"Zheng","sequence":"first","affiliation":[{"name":"Master of Medicine, Zhejiang University School of Medicine , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi Jiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Doctor of Medicine, Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0391-9963","authenticated-orcid":false,"given":"Hui","family":"Yan","sequence":"additional","affiliation":[{"name":"Doctor of Medicine, Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University , Zhejiang , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"2022070817475466000_ytac239-B1","first-page":"323","volume-title":"WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues","author":"Swerdlow","year":"2017","edition":"4th edn"},{"key":"2022070817475466000_ytac239-B2","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1182\/blood-2019-01-893339","article-title":"Viral, immunologic, and clinical features of primary effusion lymphoma","volume":"133","author":"Lurain","year":"2019","journal-title":"Blood"},{"key":"2022070817475466000_ytac239-B3","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1002\/dc.24371","article-title":"Primary effusion lymphoma in human immune deficiency (HIV)-negative non-organ transplant immunocompetent patients","volume":"48","author":"Yuan","year":"2020","journal-title":"Diagn Cytopathol"},{"key":"2022070817475466000_ytac239-B4","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1093\/qjmed\/hcu232","article-title":"Primary effusion lymphoma in the absence of HIV infection\u2013clinical presentation and management","volume":"108","author":"Klepfish","year":"2015","journal-title":"QJM Int J Med"},{"key":"2022070817475466000_ytac239-B5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1097\/QCO.0b013e3283486d04","article-title":"Human herpesvirus 8 -associated neoplasmas: the roles of viral replication and antiviral treatment","volume":"24","author":"Gantt","year":"2012","journal-title":"Curr Opin Infect Dis"},{"key":"2022070817475466000_ytac239-B6","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1093\/eurheartj\/ehv318","article-title":"2015 ESC guidelines for the diagnosis and management of pericardial diseases: the task force for the diagnosis and management of pericardial diseases of the European Society of Cardiology (ESC) endorsed by: the European Association for Cardio-Thoracic Surgery (EACTS)","volume":"36","author":"Adler","year":"2015","journal-title":"Eur Heart J"},{"key":"2022070817475466000_ytac239-B7","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nrg2626","article-title":"Sequencing technologies -the next generation","volume":"11","author":"Metzker","year":"2010","journal-title":"Nat Rev Genet"},{"key":"2022070817475466000_ytac239-B8","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1002\/cncy.22021","article-title":"Ancillary studies in pleural, pericardial, and peritoneal effusion cytology","volume":"126","author":"Sundling","year":"2018","journal-title":"Cancer Cytopathol"}],"container-title":["European Heart Journal - 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