{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T04:27:14Z","timestamp":1740112034059,"version":"3.37.3"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,2,23]],"date-time":"2021-02-23T00:00:00Z","timestamp":1614038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100005971","name":"German Heart Foundation","doi-asserted-by":"publisher","award":["F\/18\/16"],"award-info":[{"award-number":["F\/18\/16"]}],"id":[{"id":"10.13039\/501100005971","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>Heart failure induced by valvular cardiomyopathy occurs in a substantial proportion of patients undergoing heart valve surgery. We aimed (i) to quantify beta-adrenoceptor (beta-AR) function by measuring the inotropic effect of isoprenaline in left ventricular (LV) tissue and (ii) to correlate beta-AR-mediated inotropy with clinical markers of heart failure.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>A total of 179 LV myocardial samples were obtained from 104 consecutive patients who underwent aortic valve (AV) surgery between 2017 and 2019. Beta-ARs were stimulated by increasing the concentrations of isoprenaline, followed by a single high concentration of forskolin and calcium. Beta-AR sensitivity was estimated as the concentration to achieve half maximum effects (EC50). Maximum effect size was calculated as the relative beta-AR-mediated inotropic response compared to the force in the presence of high calcium [FISO\/Ca (%)]. In vitro data were correlated with the clinical indicators of LV disease.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>FISO\/Ca was independent of age and sex and amounted to 79.6\u2009\u00b1\u200920.5%. In a multivariate regression model, we found a significant inverse association between FISO\/Ca and preoperative left ventricular end-diastolic diameter increase per 10\u2009mm (OR \u22129.24, 95% CI \u221216.66 to \u22121.82; P\u2009=\u20090.015). Furthermore, patients with end-stage heart failure showed a strong tendency towards more severe reduction of max beta-AR response, as indicated by reduced FISO\/Ca in a multivariate model (OR \u221229.60, 95% CI \u221261.92 to 2.72; P\u2009=\u20090.055).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Our study indicates that in vitro myocardial contractility testing can quantify beta-AR dysfunction in patients with AV disease. We found a significant association between reduced beta-AR sensitivity and increased LV diameter, which may indicate a role of beta-AR dysfunction in the development of heart failure in patients with AV disease.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ejcts\/ezab004","type":"journal-article","created":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T12:36:57Z","timestamp":1610109417000},"page":"56-63","source":"Crossref","is-referenced-by-count":3,"title":["Blunted beta-adrenoceptor-mediated inotropy in valvular cardiomyopathy: another piece of the puzzle in human aortic valve disease"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3415-3385","authenticated-orcid":false,"given":"Johannes","family":"Petersen","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery, University Heart & Vascular Center Hamburg, Hamburg, Germany"},{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Kloth","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, University Heart & Vascular Center Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahria","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, University Heart & Vascular Center Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hermann","family":"Reichenspurner","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, University Heart & Vascular Center Hamburg, Hamburg, Germany"},{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bastian","family":"Geelhoed","sequence":"additional","affiliation":[{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"},{"name":"Department of Cardiology, University Heart & Vascular Center Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7170-9509","authenticated-orcid":false,"given":"Renate","family":"Schnabel","sequence":"additional","affiliation":[{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"},{"name":"Department of Cardiology, University Heart & Vascular Center Hamburg, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1750-1170","authenticated-orcid":false,"given":"Thomas","family":"Eschenhagen","sequence":"additional","affiliation":[{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"},{"name":"Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8935-5304","authenticated-orcid":false,"given":"Torsten","family":"Christ","sequence":"additional","affiliation":[{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"},{"name":"Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evaldas","family":"Girdauskas","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, University Heart & Vascular Center Hamburg, Hamburg, Germany"},{"name":"DZHK (German Centre for Cardiovascular Research), partner site Hamburg\/Kiel\/L\u00fcbeck, Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,2,23]]},"reference":[{"key":"2021071410024588600_ezab004-B1","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1007\/s11886-017-0941-z","article-title":"Challenges in Aortic Valve Stenosis: low-Flow States Diagnosis, Management, and a Review of the Current Literature","volume":"19","author":"Sherwood","year":"2017","journal-title":"Curr Cardiol Rep"},{"key":"2021071410024588600_ezab004-B2","doi-asserted-by":"crossref","first-page":"2618","DOI":"10.1093\/eurheartj\/ehx653","article-title":"Calcific aortic 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