{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:38:19Z","timestamp":1787503099238,"version":"build-2736575974"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,7,6]],"date-time":"2023-07-06T00:00:00Z","timestamp":1688601600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["FAPESP 2018\/25759-3"],"award-info":[{"award-number":["FAPESP 2018\/25759-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p\/>\n                  <jats:sec>\n                    <jats:title>OBJECTIVES<\/jats:title>\n                    <jats:p>Ischaemia and reperfusion-induced microvascular dysfunction is a serious problem encountered during a variety surgical procedures, leading to systemic inflammation and affecting remote organs, specially the lungs. 17\u03b2-Oestradiol reduces pulmonary repercussions from various acute lung injury forms. Here, we focused on the 17\u03b2-oestradiol therapeutic effects after aortic ischaemia and reperfusion (I\/R) by evaluating lung inflammation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Twenty-four Wistar rats were submitted to I\/R by insufflation of a 2-F catheter in thoracic aorta for 20\u2009min. Reperfusion took 4\u2009h and 17\u03b2-oestradiol (280\u2009\u00b5g\/kg, i.v.) was administered after 1\u2009h of reperfusion. Sham-operated rats were controls. Bronchoalveolar lavage was performed and lung samples were prepared for histopathological analysis and tissue culture (explant). Interleukin (IL)-1\u03b2, IL-10 and tumour necrosis factor-\u03b1 were quantified.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>After I\/R, higher number of leukocytes in bronchoalveolar lavage were reduced by 17\u03b2-oestradiol. The treatment also decreased leukocytes in lung tissue. I\/R increased lung myeloperoxidase expression, with reduction by 17\u03b2-oestradiol. Serum cytokine-induced neutrophil chemoattractant 1 and IL-1\u03b2 increased after I\/R and 17\u03b2-oestradiol decreased cytokine-induced neutrophil chemoattractant 1. I\/R increased IL-1\u03b2 and IL-10 in lung explants, reduced by 17\u03b2-oestradiol.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Our results showed that 17\u03b2-oestradiol treatment performed in the period of reperfusion, modulated the systemic response and the lung repercussions of I\/R by thoracic aortic occlusion. Thus, we can suggest that 17\u03b2-oestradiol might be a supplementary approach leading the lung deterioration after aortic clamping in surgical procedures.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ejcts\/ezad253","type":"journal-article","created":{"date-parts":[[2023,7,5]],"date-time":"2023-07-05T18:17:36Z","timestamp":1688581056000},"source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of the therapeutic effects of oestradiol on the systemic inflammatory response and on lung injury caused by the occlusion of the proximal descending aorta in male rats"],"prefix":"10.1093","volume":"64","author":[{"given":"Marcelo Nunes","family":"de Sousa","sequence":"first","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0398-4370","authenticated-orcid":false,"given":"Lucas Ferreira","family":"da Anuncia\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro Luiz Zonta","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Yamamoto","family":"Ricardo-da-Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0179-4976","authenticated-orcid":false,"given":"Luiz Felipe Pinho","family":"Moreira","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1690-9951","authenticated-orcid":false,"given":"Cristiano Jesus","family":"Correia","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0554-0674","authenticated-orcid":false,"given":"Ana Cristina","family":"Breithaupt-Faloppa","sequence":"additional","affiliation":[{"name":"Laboratorio de Cirurgia Cardiovascular e Fisiopatologia da Circula\u00e7\u00e3o (LIM-11), Instituto do Cora\u00e7\u00e3o (INCOR), Hospital das Clinicas da Faculdade de Medicina da Universidade de S\u00e3o Paulo , S\u00e3o Paulo,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,7,6]]},"reference":[{"key":"2026041420053290000_ezad253-B1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1053\/ejvs.2001.1522","article-title":"Cytokines and inflammatory pathways in the pathogenesis of multiple organ failure following abdominal aortic aneurysm repair","volume":"22","author":"Bown","year":"2001","journal-title":"Eur J Vasc Endovasc Surg"},{"key":"2026041420053290000_ezad253-B2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.ejcts.2008.05.024","article-title":"Addition of dextran sulfate to blood cardioplegia attenuates reperfusion injury in a porcine model of cardiopulmonary bypass","volume":"34","author":"Banz","year":"2008","journal-title":"Eur J Cardiothorac Surg"},{"key":"2026041420053290000_ezad253-B3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1006\/jsre.1998.5435","article-title":"Physiologic basis of pulmonary edema during intestinal reperfusion","volume":"80","author":"Iglesias","year":"1998","journal-title":"J Surg Res"},{"key":"2026041420053290000_ezad253-B4","first-page":"13","article-title":"Acute respiratory distress syndrome definition: do we need a change?","volume":"17","author":"Villar","year":"2011","journal-title":"Curr Opin Crit Care"},{"key":"2026041420053290000_ezad253-B5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jss.2017.07.038","article-title":"Estradiol mediates the long-lasting lung inflammation induced by intestinal ischemia and reperfusion","volume":"221","author":"Fantozzi","year":"2018","journal-title":"J Surg Res"},{"key":"2026041420053290000_ezad253-B6","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/nri.2016.90","article-title":"Sex differences in immune responses","volume":"16","author":"Klein","year":"2016","journal-title":"Nat Rev Immunol"},{"key":"2026041420053290000_ezad253-B7","doi-asserted-by":"crossref","first-page":"596376","DOI":"10.3389\/fimmu.2021.596376","article-title":"2-Methoxyestradiol protects against lung ischemia\/reperfusion injury by upregulating annexin A1 protein expression","volume":"12","author":"Liao","year":"2021","journal-title":"Front Immunol"},{"key":"2026041420053290000_ezad253-B8","doi-asserted-by":"crossref","first-page":"109311","DOI":"10.1016\/j.intimp.2022.109311","article-title":"Modulatory effects of 17\u03b2-estradiol on acute lung inflammation after total occlusion of the descending aorta in male rats","volume":"113","author":"da Anuncia\u00e7\u00e3o","year":"2022","journal-title":"Int Immunopharmacol"},{"key":"2026041420053290000_ezad253-B9","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1097\/SHK.0000000000000092","article-title":"Acute effects of estradiol on lung inflammation due to intestinal ischemic insult in male rats","volume":"41","author":"Breithaupt-Faloppa","year":"2014","journal-title":"Shock"},{"key":"2026041420053290000_ezad253-B10","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/j.jvs.2016.12.125","article-title":"17\u03b2-Estradiol prevents mesenteric injury induced by occlusion of the proximal descending aorta in male rats","volume":"67","author":"Rocha de Sousa","year":"2018","journal-title":"J Vasc Surg"},{"key":"2026041420053290000_ezad253-B11","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1046\/j.1365-2222.2003.01560.x","article-title":"Persistence of bronchopulmonary hyper-reactivity and eosinophilic lung inflammation after anti-IL-5 or -IL-13 treatment in allergic BALB\/c and IL-4Ralpha knockout mice","volume":"33","author":"Proust","year":"2003","journal-title":"Clin Exp Allergy"},{"key":"2026041420053290000_ezad253-B12","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<255::AID-PATH526>3.0.CO;2-6","article-title":"Pathophysiology of ischaemia-reperfusion injury","volume":"190","author":"Carden","year":"2000","journal-title":"J Pathol"},{"key":"2026041420053290000_ezad253-B13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1097\/SHK.0b013e31818bb7a1","article-title":"Nitric oxide mediates lung vascular permeability and lymph-borne IL-6 after an intestinal ischemic insult","volume":"32","author":"Breithaupt-Faloppa","year":"2009","journal-title":"Shock"},{"key":"2026041420053290000_ezad253-B14","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1177\/1538574407304510","article-title":"Reperfusion syndrome: cellular mechanisms of microvascular dysfunction and potential therapeutic strategies","volume":"41","author":"Girn","year":"2007","journal-title":"Vasc Endovascular Surg"},{"key":"2026041420053290000_ezad253-B15","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/ejcts\/ezab381","article-title":"Protective effects of 17\u03b2-oestradiol on coagulation and systemic inflammation after total occlusion of the descending aorta in male rats","volume":"61","author":"Sobral","year":"2022","journal-title":"Eur J Cardiothorac Surg"},{"key":"2026041420053290000_ezad253-B16","doi-asserted-by":"crossref","first-page":"e2683","DOI":"10.6061\/clinics\/2021\/e2683","article-title":"Estradiol prevented intestinal ischemia and reperfusion-induced changes in intestinal permeability and motility in male rats","volume":"76","author":"Ricardo-da-Silva","year":"2021","journal-title":"Clinics (Sao Paulo)"},{"key":"2026041420053290000_ezad253-B17","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1006\/jsre.1998.5276","article-title":"Visceral ischemia and neutrophil activation in sepsis and organ dysfunction","volume":"75","author":"Foulds","year":"1998","journal-title":"J Surg Res"},{"key":"2026041420053290000_ezad253-B18","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1152\/jappl.1993.75.6.2529","article-title":"Pulmonary microvascular injury after intestinal ischemia\u2013reperfusion: role of P-selectin","volume":"75","author":"Carden","year":"1993","journal-title":"J Appl Physiol (1985)"},{"key":"2026041420053290000_ezad253-B19","first-page":"H385","article-title":"Neutrophil elastase promotes lung microvascular injury and proteolysis of endothelial cadherins","volume":"275","author":"Carden","year":"1998","journal-title":"Am J Physiol"},{"key":"2026041420053290000_ezad253-B20","doi-asserted-by":"crossref","first-page":"359","DOI":"10.3109\/10739689709146800","article-title":"Lung neutrophil retention and injury following intestinal ischemia\u2013reperfusion","volume":"4","author":"Xiao","year":"1997","journal-title":"Microcirculation"},{"key":"2026041420053290000_ezad253-B21","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.3389\/fimmu.2018.01980","article-title":"Inefficient N2-like neutrophils are promoted by androgens during infection","volume":"9","author":"Scalerandi","year":"2018","journal-title":"Front Immunol"},{"key":"2026041420053290000_ezad253-B22","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.3389\/fimmu.2019.01078","article-title":"Cell adhesion molecules and their roles and regulation in the immune and tumor microenvironment","volume":"10","author":"Harjunp\u00e4\u00e4","year":"2019","journal-title":"Front Immunol"},{"key":"2026041420053290000_ezad253-B23","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1016\/S0891-5849(00)00223-9","article-title":"Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades","volume":"28","author":"Hubbard","year":"2000","journal-title":"Free Radic Biol Med"},{"key":"2026041420053290000_ezad253-B24","doi-asserted-by":"crossref","first-page":"18584","DOI":"10.1073\/pnas.0803945105","article-title":"Tracking the inflammatory response in stroke in vivo by sensing the enzyme myeloperoxidase","volume":"105","author":"Breckwoldt","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026041420053290000_ezad253-B25","doi-asserted-by":"crossref","first-page":"7990","DOI":"10.4049\/jimmunol.0800377","article-title":"Myeloperoxidase deficiency attenuates lipopolysaccharide-induced acute lung inflammation and subsequent cytokine and chemokine production","volume":"182","author":"Haegens","year":"2009","journal-title":"J Immunol"},{"key":"2026041420053290000_ezad253-B26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1177\/1358863X15622987","article-title":"Antithrombotic therapy in peripheral artery disease","volume":"21","author":"Foley","year":"2016","journal-title":"Vasc Med"},{"key":"2026041420053290000_ezad253-B27","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0031-6997(25)06663-3","article-title":"Nitric oxide: physiology, pathophysiology, and pharmacology","volume":"43","author":"Moncada","year":"1991","journal-title":"Pharmacol Rev"},{"key":"2026041420053290000_ezad253-B28","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1210\/er.2001-0045","article-title":"Estrogen modulation of endothelial nitric oxide synthase","volume":"23","author":"Chambliss","year":"2002","journal-title":"Endocr Rev"},{"key":"2026041420053290000_ezad253-B29","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1097\/SHK.0b013e3182a01e24","article-title":"Protective effect of estradiol on acute lung inflammation induced by an intestinal ischemic insult is dependent on nitric oxide","volume":"40","author":"Breithaupt-Faloppa","year":"2013","journal-title":"Shock"},{"key":"2026041420053290000_ezad253-B30","doi-asserted-by":"crossref","first-page":"2991","DOI":"10.1016\/j.biomaterials.2011.12.037","article-title":"The use of nano-quercetin to arrest mitochondrial damage and MMP-9 upregulation during prevention of gastric inflammation induced by ethanol in rat","volume":"33","author":"Chakraborty","year":"2012","journal-title":"Biomaterials"},{"key":"2026041420053290000_ezad253-B31","doi-asserted-by":"crossref","first-page":"1574928","DOI":"10.1155\/2018\/1574928","article-title":"Neutrophils release metalloproteinases during adhesion in the presence of insulin, but cathepsin G in the presence of glucagon","volume":"2018","author":"Fedorova","year":"2018","journal-title":"Mediators Inflamm"}],"container-title":["European Journal of Cardio-Thoracic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ejcts\/advance-article-pdf\/doi\/10.1093\/ejcts\/ezad253\/50830320\/ezad253.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ejcts\/article-pdf\/64\/3\/ezad253\/51649844\/ezad253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ejcts\/article-pdf\/64\/3\/ezad253\/51649844\/ezad253.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:09:48Z","timestamp":1776211788000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejcts\/article\/doi\/10.1093\/ejcts\/ezad253\/7220482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,6]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,9,7]]}},"URL":"https:\/\/doi.org\/10.1093\/ejcts\/ezad253","relation":{},"ISSN":["1873-734X"],"issn-type":[{"value":"1873-734X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,9,1]]},"published":{"date-parts":[[2023,7,6]]},"article-number":"ezad253"}}