{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:44:57Z","timestamp":1769604297421,"version":"3.49.0"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T00:00:00Z","timestamp":1721865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Foundation for Research and Promotion of Endoscopy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>This study evaluated the effect of intratracheal administration of basic fibroblast growth factor (bFGF) on tracheal healing following implantation of a novel layered polyglycolic acid (PGA) material to replace a critical-size defect in rat trachea.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>A critical-size defect in the rat cervical trachea was covered with PGA. Distilled water (DW) or 3.125, 6.25, 12.5 or 25\u2009\u00b5g bFGF was administered into the trachea for 2\u2009weeks (n\u2009=\u20096 for each of 5 groups). Regenerated areas of cilia, ciliary beat frequency and ciliary transport function (CTF) in the centre of the PGA were measured. To examine potential side effects of intratracheal administration of bFGF, the right lower lobe was pathologically evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>All rats survived during the study period. Histological examination showed ciliated epithelization on the PGA material after 2\u2009weeks. Bronchoscopy revealed stenosis due to granulation following administration of high concentrations of bFGF (12.5 and 25\u2009\u00b5g). Compared with the DW group, groups administered 3.125, 6.25, 12.5 and 25\u2009\u00b5g bFGF had significantly larger areas of regenerated cilia (15.2%, 27.0%, 41.3%, 33.1% and 31.0%, respectively; P\u2009=\u20090.00143), improved ciliary beat frequency (7.10, 8.18, 10.10, 9.50 and 9.50\u2009Hz, respectively), and improved CTS (6.40, 9.54, 16.89, 16.41 and 14.29\u2009\u00b5m\/sec, respectively). Pathological examination of the right lower lobe revealed pulmonary fibrosis and hyperplasia with high concentrations of bFGF (12.5 and 25\u2009\u00b5g).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Intratracheal administration of bFGF effectively promoted tracheal regeneration at an optimal dose of 6.25\u2009\u00b5g following implantation of an artificial trachea.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ejcts\/ezae284","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:53:03Z","timestamp":1721897583000},"source":"Crossref","is-referenced-by-count":1,"title":["Promoting successful healing of artificial trachea by intratracheal administration of basic fibroblast growth factor"],"prefix":"10.1093","volume":"66","author":[{"given":"Yoshitake","family":"Murata","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4220-969X","authenticated-orcid":false,"given":"Yojiro","family":"Yutaka","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takamasa","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rieko","family":"Hirata","sequence":"additional","affiliation":[{"name":"Central Research Laboratories, Kureha Corporation , Fukushima, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumiko","family":"Ibi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Statistics and Bioinformatics, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Hidaka","sequence":"additional","affiliation":[{"name":"Department of Biomedical Statistics and Bioinformatics, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Yoshizawa","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology, Nara Medical University , Nara, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yo","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Omori","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7262-6000","authenticated-orcid":false,"given":"Hiroshi","family":"Date","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Kyoto University Graduate School of Medicine , Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,7,25]]},"reference":[{"key":"2024080705165947000_ezae284-B1","doi-asserted-by":"crossref","first-page":"846632","DOI":"10.3389\/fbioe.2022.846632","article-title":"The growing medical need for tracheal replacement: reconstructive strategies should overcome their limits","volume":"10","author":"Adamo","year":"2022","journal-title":"Front Bioeng Biotechnol"},{"key":"2024080705165947000_ezae284-B2","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.ejcts.2006.08.023","article-title":"Tissue-engineered trachea: history, 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