{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:52:19Z","timestamp":1762102339330},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:00:00Z","timestamp":1551139200000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p \/>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>We aimed to compare the intracellular proteome of ascending aortas from patients with stenotic bicuspid (BAV) and tricuspid aortic valves (TAV) to identify BAV-specific pathogenetic mechanisms of aortopathy and to verify the previously reported asymmetric expression of BAV aortopathy [concentrated at the convexity (CVX)] in its \u2018ascending phenotype\u2019 form.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>Samples were collected from the CVX and concavity sides of non-aneurysmal ascending aortas in 26 TAV and 26 BAV patients undergoing stenotic aortic valve replacement. Aortic lysates were subjected to cellular protein enrichment by subfractionation, and to proteome comparison by 2-dimensional fluorescence difference in-gel electrophoresis. Differentially regulated protein spots were identified by liquid chromatography\u2013tandem mass spectrometry and analysed in silico. Selected results were verified by immunofluorescence and reverse transcription-polymerase chain reaction.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>In BAV samples, 52 protein spots were differentially regulated versus TAV samples at the CVX and 10 spots at the concavity: liquid chromatography\u2013tandem mass spectrometry identified 35 and 10 differentially regulated proteins, respectively. Charge trains of individual proteins (e.g. annexins) suggested the presence of post-translational modifications possibly modulating their activity. At the CVX, 37 of the 52 different protein spots showed decreased expression in BAV versus TAV. The affected biological pathways included those involved in smooth muscle cell contractile phenotype, metabolism and cell stress.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>The observed differential proteomics profiles may have a significant impact on the pathogenesis of the aortopathy, pointing the way for further studies. At a preaneurysmal stage, an aorta with BAV shows more protein expression changes and potentially more post-translational modifications at the CVX of the ascending aorta than at the concavity, compared to that of TAV.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ejcts\/ezz032","type":"journal-article","created":{"date-parts":[[2019,1,27]],"date-time":"2019-01-27T04:11:00Z","timestamp":1548562260000},"page":"458-469","source":"Crossref","is-referenced-by-count":8,"title":["Locally different proteome in aortas from patients with stenotic tricuspid and bicuspid aortic valves\u2020"],"prefix":"10.1093","volume":"56","author":[{"given":"Amalia","family":"Forte","sequence":"first","affiliation":[{"name":"Department of Translational Medical Sciences, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoke","family":"Yin","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, King\u2019s British Heart Foundation Centre, King\u2019s College London, London, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marika","family":"Fava","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, King\u2019s British Heart Foundation Centre, King\u2019s College London, London, UK"},{"name":"Division of Cardiology, Department of Medicine, Cardiovascular Research Center, Mount Sinai Hospital, New York, NY, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ciro","family":"Bancone","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilena","family":"Cipollaro","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Universit\u00e0 della Campania \u201cL. Vanvitelli\u201d, Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"De Feo","sequence":"additional","affiliation":[{"name":"Department of Translational Medical Sciences, Universit\u00e0 della Campania \u201cL. 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