{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:54:05Z","timestamp":1774504445121,"version":"3.50.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T00:00:00Z","timestamp":1567036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p\/>\n               <jats:sec>\n                  <jats:title>OBJECTIVES<\/jats:title>\n                  <jats:p>Different types of patch materials are used for aortic valve repair in children with congenital aortic valve disease to avoid early valve replacement. CardioCel\u00a9 (Admedus, Toowong, QLD, Australia) consists of bovine pericardium treated with the ADAPT method (Admedus\u2019 proprietary tissue engineering process).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>Our goal was to describe tissue reactions in 12 explanted aortic valve leaflet specimens (augmented or replaced with CardioCel patch material) (11 explanted surgically, 1 autopsy). Explantation was performed during reoperation after aortic valve repair, necessitated by aortic valve stenosis in 5, aortic valve insufficiency in 2, combined aortic valve lesions in 3 patients and endocarditis in 1 patient. One patient died of sudden left heart failure 28\u2009months after aortic valve repair. At the last documented follow-up of this patient at 22\u2009months, he showed mild aortic valve stenosis and insufficiency. Implantation time (time between implantation and explantation) of CardioCel patches was a median of 25 (range 11\u201347) months. Explants were examined using a uniform protocol with methylmethacrylate and\/or paraffin embedding after fixation in formalin. Besides standard histological staining, von Kossa (for identification of calcifications) and immunohistochemical stains were applied with antibodies specific for muscular, inflammatory and connective tissue component antigens. Findings regarding the extent of appositional growth on top of the patch consisting of fibroblasts and extracellular matrix components, calcification, and inflammation were rated using a 4-grade scale (G0 no\/G1 few\/G2 moderate\/G3 massive).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>Superficial endothelialization was demonstrated in all patients by immunohistochemical analysis. Nine specimens showed mild inflammatory cell infiltration (G1) (G2: n\u2009=\u20093). Significant appositional growth on top of the patch due to addition of fibroblasts and extracellular matrix components, was seen in all specimens (G1: n\u2009=\u20091; G2: n\u2009=\u20097; G3: n\u2009=\u20094). Ten of 12 samples with implant times of 23\u2009months or longer revealed calcifications (G1: n\u2009=\u20096; G2: n\u2009=\u20093; G3: n\u2009=\u20091). Two specimens with the shortest implant times (11 and 20\u2009months) showed no calcifications (G0). Thrombus apposition with granulocyte infiltration was demonstrated in the specimen of the patient with endocarditis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>In our cohort, all CardioCel patches used for aortic valve repair in patients with congenital heart disease demonstrated appositional growth of fibroblasts and extracellular matrix components, and calcification after an implant time of at least 23\u2009months.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ejcts\/ezz228","type":"journal-article","created":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T07:08:59Z","timestamp":1564470539000},"page":"1170-1177","source":"Crossref","is-referenced-by-count":17,"title":["ADAPT-treated pericardium for aortic valve reconstruction in congenital heart disease: histological analysis of a series of human explants"],"prefix":"10.1093","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1111-2521","authenticated-orcid":false,"given":"Sarah","family":"Nordmeyer","sequence":"first","affiliation":[{"name":"Department of Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4358-7855","authenticated-orcid":false,"given":"Johanna","family":"Kretzschmar","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Murin","sequence":"additional","affiliation":[{"name":"Department of Surgery for Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi-Young","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Surgery for Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudi","family":"Foth","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology and Intensive Care Medicine, University Medical Center G\u00f6ttingen, G\u00f6ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Schlichting","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sana Klinikum Lichtenberg, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanislav","family":"Ovroutski","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Photiadis","sequence":"additional","affiliation":[{"name":"Department of Surgery for Congenital Heart Disease and Paediatric Cardiology, Deutsches Herzzentrum Berlin, Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4110-7694","authenticated-orcid":false,"given":"Matthias","family":"Sigler","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology and Intensive Care Medicine, University Medical Center G\u00f6ttingen, G\u00f6ttingen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,8,29]]},"reference":[{"key":"2019111904003817800_ezz228-B1","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.jtcvs.2009.12.015","article-title":"Aortic cusp extension valvuloplasty with or without tricuspidization in children and adolescents: long-term results and freedom from aortic valve replacement","volume":"139","author":"Polimenakos","year":"2010","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2019111904003817800_ezz228-B2","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1016\/j.athoracsur.2013.07.130","article-title":"Outcomes after operations for bicuspid aortic valve disease in the pediatric population","volume":"96","author":"Siddiqui","year":"2013","journal-title":"Ann Thorac 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labelling","volume":"111","author":"Quentin","year":"2009","journal-title":"Acta Histochemica"},{"key":"2019111904003817800_ezz228-B15","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1053\/j.semtcvs.2017.05.017","article-title":"Histologic evaluation of explanted tissue-engineered bovine pericardium (CardioCel)","volume":"29","author":"Prabhu","year":"2017","journal-title":"Semin Thorac Cardiovasc Surg"},{"key":"2019111904003817800_ezz228-B16","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1053\/j.semtcvs.2017.09.013","article-title":"Calcification or not. This is the question. A one year study of bovine pericardial vascular patches (CardioCel\u00ae) in minipigs","volume":"30","author":"Salameh","year":"2018","journal-title":"Semin Thorac Cardiovasc Surg"},{"key":"2019111904003817800_ezz228-B17","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1093\/icvts\/ivx295","article-title":"Initial 2-year results of CardioCel\u00ae patch implantation in children","volume":"26","author":"Pavy","year":"2018","journal-title":"Interact CardioVasc Thorac Surg"},{"key":"2019111904003817800_ezz228-B18","doi-asserted-by":"crossref","DOI":"10.1177\/2150135118769322","article-title":"Infective endocarditis associated with varicella zoster virus following aortic valve repair","author":"Kostolny","year":"2018","journal-title":"World J Pediatr Congenit Heart Surg"},{"key":"2019111904003817800_ezz228-B19","doi-asserted-by":"crossref","first-page":"e0204643.","DOI":"10.1371\/journal.pone.0204643","article-title":"Congenital heart defect repair with ADAPT tissue engineered pericardium scaffold: an early-stage health economic model","volume":"13","author":"Velickovic","year":"2018","journal-title":"PLoS One"}],"container-title":["European Journal of Cardio-Thoracic Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ejcts\/article-pdf\/56\/6\/1170\/30960767\/ezz228.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ejcts\/article-pdf\/56\/6\/1170\/30960767\/ezz228.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,19]],"date-time":"2019-11-19T04:01:32Z","timestamp":1574136092000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejcts\/article\/56\/6\/1170\/5556364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,29]]},"references-count":19,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2019,8,29]]},"published-print":{"date-parts":[[2019,12,1]]}},"URL":"https:\/\/doi.org\/10.1093\/ejcts\/ezz228","relation":{},"ISSN":["1010-7940","1873-734X"],"issn-type":[{"value":"1010-7940","type":"print"},{"value":"1873-734X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,12]]},"published":{"date-parts":[[2019,8,29]]}}}