{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:10:28Z","timestamp":1790701828077,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T00:00:00Z","timestamp":1574899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Boston Children\u2019s Hospital Anesthesia Foundation"},{"name":"Ryan Family Endowment, the Cardiac Conduction Fund"},{"name":"Sidman Family Foundation"},{"name":"Michael B. Rukin Charitable Foundation"},{"name":"Kenneth C. Griffin Charitable Research Fund"},{"name":"Boston Investment Council"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p\/>\n                  <jats:sec>\n                    <jats:title>OBJECTIVES<\/jats:title>\n                    <jats:p>Type 2 diabetes causes mitochondrial dysfunction, which increases myocardial susceptibility to ischaemia\u2013reperfusion injury. We investigated the efficacy of transplantation of mitochondria isolated from diabetic or non-diabetic donors in providing cardioprotection from warm global ischaemia and reperfusion in the diabetic rat heart.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Ex vivo perfused hearts from Zucker diabetic fatty (ZDF fa\/fa) rats (n\u2009=\u20096 per group) were subjected to 30\u2009min of warm global ischaemia and 120\u2009min reperfusion. Immediately prior to reperfusion, vehicle alone (VEH) or vehicle containing mitochondria isolated from either ZDF (MTZDF) or non-diabetic Zucker lean (ZL +\/?) (MTZL) skeletal muscle were delivered to the coronary arteries via the aortic cannula.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Following 30-min global ischaemia and 120-min reperfusion, left ventricular developed pressure was significantly increased in MTZDF and MTZL groups compared to VEH group (MTZDF: 92.8\u2009\u00b1\u20095.2\u2009mmHg vs MTZL: 110.7\u2009\u00b1\u20092.4\u2009mmHg vs VEH: 44.3\u2009\u00b1\u20095.9\u2009mmHg; P\u2009&amp;lt;\u20090.01 each); and left ventricular end-diastolic pressure was significantly decreased (MTZDF 12.1\u2009\u00b1\u20091.3\u2009mmHg vs MTZL 8.6\u2009\u00b1\u20090.8\u2009mmHg vs VEH: 18.6\u2009\u00b1\u20091.5\u2009mmHg; P\u2009=\u20090.016 for MTZDF vs VEH and P\u2009&amp;lt;\u20090.01 for MTZL vs VEH). Total tissue ATP content was significantly increased in both MT groups compared to VEH group (MTZDF: 18.9\u2009\u00b1\u20091.5\u2009mmol\/mg protein\/mg tissue vs MTZL: 28.1\u2009\u00b1\u20092.3\u2009mmol\/mg protein\/mg tissue vs VEH: 13.1\u2009\u00b1\u20090.5\u2009mmol\/mg protein\/mg tissue; P\u2009=\u20090.018 for MTZDF vs VEH and P\u2009&amp;lt;\u20090.01 for MTZL vs VEH). Infarct size was significantly decreased in the MT groups (MTZDF: 11.8\u2009\u00b1\u20090.7% vs MTZL: 9.9\u2009\u00b1\u20090.5% vs VEH: 52.0\u2009\u00b1\u20091.4%; P\u2009&amp;lt;\u20090.01 each).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Mitochondrial transplantation significantly enhances post-ischaemic myocardial functional recovery and significantly decreases myocellular injury in the diabetic heart.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ejcts\/ezz326","type":"journal-article","created":{"date-parts":[[2019,11,4]],"date-time":"2019-11-04T23:51:46Z","timestamp":1572911506000},"page":"836-845","source":"Crossref","is-referenced-by-count":85,"title":["Mitochondrial transplantation for myocardial protection in diabetic hearts"],"prefix":"10.1093","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4128-7111","authenticated-orcid":false,"given":"Ilias P","family":"Doulamis","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5495-6919","authenticated-orcid":false,"given":"Alvise","family":"Guariento","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3659-8476","authenticated-orcid":false,"given":"Thomas","family":"Duignan","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9468-6810","authenticated-orcid":false,"given":"Arzoo","family":"Orfany","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Kido","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3610-6942","authenticated-orcid":false,"given":"David","family":"Zurakowski","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro J","family":"del Nido","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D","family":"McCully","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, Boston Children\u2019s Hospital, Harvard Medical School, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,11,28]]},"reference":[{"key":"2020042307455731900_ezz326-B1","doi-asserted-by":"crossref","first-page":"2239","DOI":"10.1016\/S0140-6736(17)30058-2","article-title":"Type 2 diabetes","volume":"389","author":"Chatterjee","year":"2017","journal-title":"Lancet"},{"key":"2020042307455731900_ezz326-B2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12933-017-0628-1","article-title":"Effects of hypoglycemia on myocardial susceptibility to ischemia-reperfusion injury and preconditioning in hearts from rats with and without type 2 diabetes","volume":"16","author":"P\u00e6lestik","year":"2017","journal-title":"Cardiovasc Diabetol"},{"key":"2020042307455731900_ezz326-B3","doi-asserted-by":"crossref","first-page":"H966","DOI":"10.1152\/ajpheart.00883.2012","article-title":"Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury","volume":"304","author":"Masuzawa","year":"2013","journal-title":"Am J Physiol Circ Physiol"},{"key":"2020042307455731900_ezz326-B4","doi-asserted-by":"crossref","first-page":"H94","DOI":"10.1152\/ajpheart.00567.2008","article-title":"Injection of isolated mitochondria during early reperfusion for cardioprotection","volume":"296","author":"McCully","year":"2009","journal-title":"Am J Physiol Circ Physiol"},{"key":"2020042307455731900_ezz326-B5","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1016\/j.jtcvs.2016.10.077","article-title":"Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia\/reperfusion","volume":"153","author":"Kaza","year":"2017","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2020042307455731900_ezz326-B6","doi-asserted-by":"crossref","first-page":"e0160889","DOI":"10.1371\/journal.pone.0160889","article-title":"Intracoronary delivery of mitochondria to the ischemic heart for cardioprotection","volume":"11","author":"Cowan","year":"2016","journal-title":"PLoS One"},{"key":"2020042307455731900_ezz326-B7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.jacbts.2018.10.002","article-title":"SGLT2 inhibitor, canagliflozin, attenuates myocardial infarction in the diabetic and nondiabetic heart","volume":"4","author":"Lim","year":"2019","journal-title":"JACC Basic Transl Sci"},{"key":"2020042307455731900_ezz326-B8","first-page":"2","article-title":"Rapid isolation and purification of mitochondria for transplantation by tissue dissociation and differential filtration","volume":"8","author":"Preble","year":"2014","journal-title":"J Vis Exp"},{"key":"2020042307455731900_ezz326-B9","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1242\/bio.201511478","article-title":"Actin-dependent mitochondrial internalization in cardiomyocytes: evidence for rescue of mitochondrial function","volume":"4","author":"Pacak","year":"2015","journal-title":"Biol Open"},{"key":"2020042307455731900_ezz326-B10","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.jtcvs.2017.02.018","article-title":"Autologous mitochondrial transplantation for dysfunction after ischemia-reperfusion injury","volume":"154","author":"Emani","year":"2017","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2020042307455731900_ezz326-B11","doi-asserted-by":"crossref","first-page":"169","DOI":"10.21037\/tp.2018.02.02","article-title":"Mitochondrial transplantation: applications for pediatric patients with congenital heart disease","volume":"7","author":"Emani","year":"2018","journal-title":"Transl Pediatr"},{"key":"2020042307455731900_ezz326-B12","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.mito.2017.03.004","article-title":"Mitochondrial transplantation: from animal models to clinical use in humans","volume":"34","author":"McCully","year":"2017","journal-title":"Mitochondrion"},{"key":"2020042307455731900_ezz326-B13","article-title":"Mitochondrial transplantation ameliorates acute limb ischemia","author":"Orfany","year":"2019","journal-title":"J Vasc Surg"},{"key":"2020042307455731900_ezz326-B14","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.healun.2018.09.025","article-title":"Mitochondrial transplantation prolongs cold ischemia time in murine heart transplantation","volume":"38","author":"Moskowitzova","year":"2019","journal-title":"J Heart Lung Transplant"},{"key":"2020042307455731900_ezz326-B15","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1007\/978-3-319-55330-6_31","article-title":"Mitochondrial transplantation in myocardial ischemia and reperfusion injury","volume":"982","author":"Shin","year":"2017","journal-title":"Adv Exp Med Biol"},{"key":"2020042307455731900_ezz326-B16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/s40169-016-0095-4","article-title":"Mitochondrial transplantation for therapeutic use","volume":"5","author":"McCully","year":"2016","journal-title":"Clin Transl Med"},{"key":"2020042307455731900_ezz326-B17","doi-asserted-by":"crossref","first-page":"H966","DOI":"10.1152\/ajpheart.00883.2012","article-title":"Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury","volume":"304","author":"Masuzawa","year":"2013","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"2020042307455731900_ezz326-B18","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.mito.2018.03.002","article-title":"Alloreactivity and allorecognition of syngeneic and allogeneic mitochondria","volume":"46","author":"Ramirez-Barbieri","year":"2019","journal-title":"Mitochondrion"},{"key":"2020042307455731900_ezz326-B19"},{"key":"2020042307455731900_ezz326-B20","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-62703-068-7_8","article-title":"Diabetes in Zucker diabetic fatty rat","volume":"933","author":"Shiota","year":"2012","journal-title":"Methods Mol Biol"},{"key":"2020042307455731900_ezz326-B21","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal Biochem"},{"key":"2020042307455731900_ezz326-B22","doi-asserted-by":"crossref","DOI":"10.1016\/j.jtcvs.2019.06.111","article-title":"Preischemic autologous mitochondrial transplantation by intracoronary injection for myocardial protection","author":"Guariento","year":"2019","journal-title":"J Thorac Cardiovasc Surg"},{"key":"2020042307455731900_ezz326-B23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.yjmcc.2015.12.020","article-title":"Ischemia reperfusion injury, ischemic conditioning and diabetes mellitus","volume":"91","author":"Lejay","year":"2016","journal-title":"J Mol Cell Cardiol"},{"key":"2020042307455731900_ezz326-B24","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1007\/s00125-004-1514-4","article-title":"Ischaemic preconditioning does not protect the heart in obese and lean animal models of type 2 diabetes","volume":"47","author":"Kristiansen","year":"2004","journal-title":"Diabetologia"},{"key":"2020042307455731900_ezz326-B25","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1159\/000373947","article-title":"Increased oxidative stress and mitochondrial dysfunction in Zucker diabetic rat liver and brain","volume":"35","author":"Raza","year":"2015","journal-title":"Cell Physiol Biochem"},{"key":"2020042307455731900_ezz326-B26","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.bbrc.2010.09.115","article-title":"ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects","volume":"402","author":"Minet","year":"2010","journal-title":"Biochem Biophys Res Commun"},{"key":"2020042307455731900_ezz326-B27","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.cardiores.2007.03.025","article-title":"Gender-based differences in mechanisms of protection in myocardial ischemia-reperfusion injury","volume":"75","author":"Murphy","year":"2007","journal-title":"Cardiovasc Res"}],"container-title":["European Journal of Cardio-Thoracic Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/ejcts\/article-pdf\/57\/5\/836\/33117241\/ezz326.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/ejcts\/article-pdf\/57\/5\/836\/33117241\/ezz326.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T09:38:47Z","timestamp":1695375527000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ejcts\/article\/57\/5\/836\/5647332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,28]]},"references-count":27,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,11,28]]},"published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/ejcts\/ezz326","relation":{},"ISSN":["1010-7940","1873-734X"],"issn-type":[{"value":"1010-7940","type":"print"},{"value":"1873-734X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5]]},"published":{"date-parts":[[2019,11,28]]}}}