{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:30:07Z","timestamp":1788877807439,"version":"build-2803163510"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,19]],"date-time":"2024-01-19T00:00:00Z","timestamp":1705622400000},"content-version":"vor","delay-in-days":18,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Fund Congenital Adrenal Hyperplasia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Classic androgens such as dehydroepiandrosterone, androstenedione, and testosterone are generally measured for diagnosis and treatment monitoring in children and adolescents with hyperandrogenism, as can occur in congenital adrenal hyperplasia, premature pubarche, or polycystic ovarian syndrome. However, adrenally-derived 11-oxygenated androgens also contribute to the androgen pool and should therefore be considered in clinical management. Nevertheless, paediatric reference intervals are lacking. Therefore, we developed a serum assay to establish reference intervals for four 11-oxygenated androgens in addition to four classic androgens.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>Reference interval study for serum 11-oxygenated androgens in children.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We developed and validated a sensitive liquid chromatography-tandem mass spectrometry assay and quantified eight serum androgens, including four 11-oxygenated androgens, in serum of 256 healthy children (aged 0-17 years old). An age-dependency for all androgens was observed, and therefore we divided the cohort based on age (prepubertal [n = 133; 94 boys, 39 girls] and pubertal [n = 123; 52 boys, 71 girls]) to compute reference intervals (2.5th-97.5th percentiles).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In the prepubertal group, there was no significant sex-difference for any of the measured androgens. In the pubertal group, androstenedione, testosterone, and dihydrotestosterone showed a significant difference between boys and girls. In contrast, adrenal androgens dehydroepiandrosterone, 11-hydroxyandrostenedione, 11-ketoandrostenedione, 11-hydroxytestosterone, and 11-ketotestosterone did not.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>We developed and validated an assay for 11-oxygenated androgens, in addition to four classic androgens and established reference intervals. This enables a comprehensive evaluation of serum androgen status in children with clinical symptoms of hyperandrogenism.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ejendo\/lvae008","type":"journal-article","created":{"date-parts":[[2024,1,20]],"date-time":"2024-01-20T14:47:25Z","timestamp":1705762045000},"page":"96-103","source":"Crossref","is-referenced-by-count":13,"title":["Reference intervals for serum 11-oxygenated androgens in children"],"prefix":"10.1093","volume":"190","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1439-236X","authenticated-orcid":false,"given":"Bas P H","family":"Adriaansen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"},{"name":"Department of Paediatric Endocrinology, Amalia Children\u2019s Hospital, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra E","family":"Oude Alink","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorine W","family":"Swinkels","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2139-9076","authenticated-orcid":false,"given":"Mariska A M","family":"Schr\u00f6der","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"},{"name":"Department of Paediatric Endocrinology, Amalia Children\u2019s Hospital, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1930-6638","authenticated-orcid":false,"given":"Paul N","family":"Span","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred C G J","family":"Sweep","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0181-0403","authenticated-orcid":false,"given":"Hedi L","family":"Claahsen-van der Grinten","sequence":"additional","affiliation":[{"name":"Department of Paediatric Endocrinology, Amalia Children\u2019s Hospital, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonius E","family":"van Herwaarden","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Radboud University Medical Centre , 6525GA, Nijmegen , the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,19]]},"reference":[{"issue":"12","key":"2024013023092660300_lvae008-B1","doi-asserted-by":"crossref","first-page":"4585","DOI":"10.1210\/jc.2016-2864","article-title":"Age-dependent increases in adrenal cytochrome b5 and serum 5-androstenediol-3-sulfate","volume":"101","author":"Rege","year":"2016","journal-title":"J Clin Endocrinol Metab"},{"issue":"12","key":"2024013023092660300_lvae008-B2","first-page":"4589","article-title":"11-Ketotestosterone is the dominant circulating bioactive androgen during normal and premature adrenarche","volume":"103","author":"Rege","year":"2018","journal-title":"J Clin 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