{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T13:09:16Z","timestamp":1779109756898,"version":"3.51.4"},"reference-count":10,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2001,4,1]],"date-time":"2001-04-01T00:00:00Z","timestamp":986083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO Rep"],"published-print":{"date-parts":[[2001,4,1]]},"DOI":"10.1093\/embo-reports\/kve067","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:49:38Z","timestamp":1027709378000},"page":"324-329","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Stoichiometry and kinetics of transport vesicle fusion with Golgi membranes"],"prefix":"10.1038","volume":"2","author":[{"given":"Joachim","family":"Ostermann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2001,4,1]]},"reference":[{"key":"BFEMBR444_CR1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/0092-8674(84)90019-9","volume":"39","author":"W.E. Balch","year":"1984","unstructured":"Balch, W.E., Dunphy, W.G., Braell, W.A. and Rothman, J.E. (1984) Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N\u2010acetylglucosamine. Cell, 39, 405\u2013416.","journal-title":"Cell"},{"key":"BFEMBR444_CR2","doi-asserted-by":"crossref","first-page":"23438","DOI":"10.1016\/S0021-9258(18)54516-6","volume":"266","author":"M.I. Colombo","year":"1991","unstructured":"Colombo, M.I., Gonzalo, S., Weidman, P. and Stahl, P. (1991) Characterization of trypsin\u2010sensitive factor(s) required for endosome\u2013endosome fusion. J. Biol. Chem., 266, 23438\u201323445.","journal-title":"J. Biol. Chem."},{"key":"BFEMBR444_CR3","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1073\/pnas.77.7.3870","volume":"77","author":"E. Fries","year":"1980","unstructured":"Fries, E. and Rothman, J.E. (1980) Transport of vesicular stomatitis virus glycoprotein in a cell\u2010free extract. Proc. Natl Acad. Sci. USA, 77, 3870\u20133874.","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFEMBR444_CR4","doi-asserted-by":"crossref","first-page":"6111","DOI":"10.1021\/bi00141a022","volume":"31","author":"R.R. Hiebsch","year":"1992","unstructured":"Hiebsch, R.R. and Wattenberg, B.W. (1992) Vesicle fusion in protein transport through the Golgi in vitro does not involve long\u2010lived prefusion intermediates. A reassessment of the kinetics of transport as measured by glycosylation. Biochemistry, 31, 6111\u20136118.","journal-title":"Biochemistry"},{"key":"BFEMBR444_CR5","doi-asserted-by":"crossref","first-page":"4935","DOI":"10.1093\/emboj\/18.18.4935","volume":"18","author":"J. Lanoix","year":"1999","unstructured":"Lanoix, J., Ouwendijk, J., Lin, C.C., Stark, A., Love, H.D., Ostermann, J. and Nilsson, T. (1999) GTP hydrolysis by arf\u20101 mediates sorting and concentration of Golgi resident enzymes into functional COP I vesicles. EMBO J., 18, 4935\u20134948.","journal-title":"EMBO J."},{"key":"BFEMBR444_CR6","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1083\/jcb.147.7.1457","volume":"147","author":"C.C. Lin","year":"1999","unstructured":"Lin, C.C., Love, H.D., Gushue, J.N., Bergeron, J.J. and Ostermann, J. (1999) ER\/Golgi intermediates acquire Golgi enzymes by brefeldin A\u2010sensitive retrograde transport in vitro. J. Cell Biol., 147, 1457\u20131472.","journal-title":"J. Cell Biol."},{"key":"BFEMBR444_CR7","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1083\/jcb.140.3.541","volume":"140","author":"H.D. Love","year":"1998","unstructured":"Love, H.D., Lin, C.C., Short, C.S. and Ostermann, J. (1998) Isolation of functional Golgi\u2010derived vesicles with a possible role in retrograde transport. J. Cell Biol., 140, 541\u2013551.","journal-title":"J. Cell Biol."},{"key":"BFEMBR444_CR8","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/0092-8674(93)90545-2","volume":"75","author":"J. Ostermann","year":"1993","unstructured":"Ostermann, J., Orci, L., Tani, K., Amherdt, M., Ravazzola, M., Elazar, Z. and Rothman, J.E. (1993) Stepwise assembly of functionally active transport vesicles. Cell, 75, 1015\u20131025.","journal-title":"Cell"},{"key":"BFEMBR444_CR9","doi-asserted-by":"crossref","first-page":"29233","DOI":"10.1074\/jbc.M005316200","volume":"275","author":"A. Porat","year":"2000","unstructured":"Porat, A. and Elazar, Z. (2000) Regulation of intra\u2010Golgi membrane transport by calcium. J. Biol. Chem., 275, 29233\u201329237.","journal-title":"J. Biol. Chem."},{"key":"BFEMBR444_CR10","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/0092-8674(84)90508-7","volume":"38","author":"J. Saraste","year":"1984","unstructured":"Saraste, J. and Kuismanen, E. (1984) Pre\u2010 and post\u2010Golgi vacuoles operate in the transport of Semliki Forest virus membrane glycoproteins to the cell surface. Cell, 38, 535\u2013549.","journal-title":"Cell"}],"container-title":["The EMBO Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1093%2Fembo-reports%2Fkve067","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1093\/embo-reports\/kve067.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1093\/embo-reports\/kve067","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1093\/embo-reports\/kve067\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.embopress.org\/doi\/pdf\/10.1093\/embo-reports\/kve067","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1093\/embo-reports\/kve067.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T12:28:09Z","timestamp":1779107289000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1093\/embo-reports\/kve067"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,1]]},"references-count":10,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,4,1]]}},"alternative-id":["BFEMBR444"],"URL":"https:\/\/doi.org\/10.1093\/embo-reports\/kve067","relation":{},"ISSN":["1469-221X","1469-3178"],"issn-type":[{"value":"1469-221X","type":"print"},{"value":"1469-3178","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,1]]},"assertion":[{"value":"12 October 2000","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 January 2001","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 February 2001","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 April 2001","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}