{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T19:09:24Z","timestamp":1779304164253,"version":"3.51.4"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"20","license":[{"start":{"date-parts":[[2000,10,16]],"date-time":"2000-10-16T00:00:00Z","timestamp":971654400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2000,10,16]],"date-time":"2000-10-16T00:00:00Z","timestamp":971654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["EMBO J"],"published-print":{"date-parts":[[2000,10,16]]},"DOI":"10.1093\/emboj\/19.20.5575","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T18:50:23Z","timestamp":1027709423000},"page":"5575-5584","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Compartmentalization of phage \u03c629 DNA replication: interaction between the primer terminal protein and the membrane\u2010associated protein p1"],"prefix":"10.1038","volume":"19","author":[{"given":"Alicia","family":"Bravo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bel\u00e9n","family":"Illana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita","family":"Salas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2000,10,16]]},"reference":[{"key":"BFEMBJ7593372_CR1","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1093\/emboj\/18.11.3164","volume":"18","author":"T Ahola","year":"1999","unstructured":"Ahola T, Lampio A, Auvinen P and K\u00e4\u00e4ri\u00e4inen L (1999) Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity. EMBO J, 18, 3164\u20133172.","journal-title":"EMBO J"},{"key":"BFEMBJ7593372_CR2","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1128\/jvi.60.3.874-879.1986","volume":"60","author":"I Barthelemy","year":"1986","unstructured":"Barthelemy I, Salas M and Mellado RP (1986) In vivo transcription of bacteriophage \u03c629 DNA: transcription initiation sites. J Virol, 60, 874\u2013879.","journal-title":"J Virol"},{"key":"BFEMBJ7593372_CR3","doi-asserted-by":"crossref","first-page":"6404","DOI":"10.1073\/pnas.82.19.6404","volume":"82","author":"L Blanco","year":"1985","unstructured":"Blanco L and Salas M (1985) Replication of phage \u03c629 DNA with purified terminal protein and DNA polymerase: synthesis of full\u2010length \u03c629 DNA. Proc Natl Acad Sci USA, 82, 6404\u20136408.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBJ7593372_CR4","doi-asserted-by":"crossref","first-page":"3983","DOI":"10.1128\/jvi.61.12.3983-3991.1987","volume":"61","author":"L Blanco","year":"1987","unstructured":"Blanco L, Prieto I, Guti\u00e9rrez J, Bernad A, L\u00e1zaro JM, Hermoso JM and Salas M (1987) Effect of NH4+ ions on \u03c629 DNA\u2013protein p3 replication: formation of a complex between the terminal protein and the DNA polymerase. J Virol, 61, 3983\u20133991.","journal-title":"J Virol"},{"key":"BFEMBJ7593372_CR5","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1006\/jmbi.1997.1032","volume":"269","author":"A Bravo","year":"1997","unstructured":"Bravo A and Salas M (1997) Initiation of bacteriophage \u03c629 DNA replication in vivo: assembly of a membrane\u2010associated multiprotein complex. J Mol Biol, 269, 102\u2013112.","journal-title":"J Mol Biol"},{"key":"BFEMBJ7593372_CR6","doi-asserted-by":"crossref","first-page":"6096","DOI":"10.1093\/emboj\/17.20.6096","volume":"17","author":"A Bravo","year":"1998","unstructured":"Bravo A and Salas M (1998) Polymerization of bacteriophage \u03c629 replication protein p1 into protofilament sheets. EMBO J, 17, 6096\u20136105.","journal-title":"EMBO J"},{"key":"BFEMBJ7593372_CR7","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1093\/nar\/18.10.2881","volume":"18","author":"A Bravo","year":"1990","unstructured":"Bravo A, Alonso JC and Trautner TA (1990) Functional analysis of the Bacillus subtilis bacteriophage SPP1 pac site. Nucleic Acids Res, 18, 2881\u20132886.","journal-title":"Nucleic Acids Res"},{"key":"BFEMBJ7593372_CR8","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1007\/BF00282215","volume":"245","author":"A Bravo","year":"1994","unstructured":"Bravo A, Hermoso JM and Salas M (1994) A genetic approach to the identification of functional amino acids in protein p6 of Bacillus subtilis phage \u03c629. Mol Gen Genet, 245, 529\u2013536.","journal-title":"Mol Gen Genet"},{"key":"BFEMBJ7593372_CR9","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/0022-2836(81)90508-8","volume":"148","author":"J Brosius","year":"1981","unstructured":"Brosius J, Dull TJ, Sleeter DD and Noller HF (1981) Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol, 148, 107\u2013127.","journal-title":"J Mol Biol"},{"key":"BFEMBJ7593372_CR10","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/S0065-3527(08)60736-8","volume":"47","author":"KW Buck","year":"1996","unstructured":"Buck KW (1996) Comparison of the replication of positive\u2010stranded RNA viruses of plants and animals. Adv Virus Res, 47, 159\u2013251.","journal-title":"Adv Virus Res"},{"key":"BFEMBJ7593372_CR11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1111\/j.1432-1033.1976.tb10512.x","volume":"66","author":"JL Carrascosa","year":"1976","unstructured":"Carrascosa JL, Camacho A, Moreno F, Jim\u00e9nez F, Mellado RP, Vi\u00f1uela E and Salas M (1976) Bacillus subtilis phage \u03c629: characterization of gene products and functions. Eur J Biochem, 66, 229\u2013241.","journal-title":"Eur J Biochem"},{"key":"BFEMBJ7593372_CR12","doi-asserted-by":"crossref","first-page":"1790","DOI":"10.1126\/science.284.5421.1790","volume":"284","author":"PR Cook","year":"1999","unstructured":"Cook PR (1999) The organization of replication and transcription. Science, 284, 1790\u20131795.","journal-title":"Science"},{"key":"BFEMBJ7593372_CR13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0378-1119(99)00249-8","volume":"236","author":"RN de Jong","year":"1999","unstructured":"de Jong RN and van der Vliet PC (1999) Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication. Gene, 236, 1\u201312.","journal-title":"Gene"},{"key":"BFEMBJ7593372_CR14","doi-asserted-by":"crossref","first-page":"31000","DOI":"10.1074\/jbc.271.48.31000","volume":"271","author":"R Freire","year":"1996","unstructured":"Freire R, Serrano M, Salas M and Hermoso JM (1996) Activation of replication origins in \u03c629\u2010related phages requires the recognition of initiation proteins to specific nucleoprotein complexes. J Biol Chem, 271, 31000\u201331007.","journal-title":"J Biol Chem"},{"key":"BFEMBJ7593372_CR15","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0042-6822(73)90330-9","volume":"52","author":"RD Ivarie","year":"1973","unstructured":"Ivarie RD and P\u00e8ne JJ (1973) DNA replication in bacteriophage \u03c629: the requirement of a viral\u2010specific product for association of \u03c629 DNA with the cell membrane of Bacillus amyloliquefaciens. Virology, 52, 351\u2013362.","journal-title":"Virology"},{"key":"BFEMBJ7593372_CR16","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/S0076-6879(82)90171-9","volume":"90","author":"OK Kellermann","year":"1982","unstructured":"Kellermann OK and Ferenci T (1982) Maltose\u2010binding protein from Escherichia coli. Methods Enzymol, 90, 459\u2013463.","journal-title":"Methods Enzymol"},{"key":"BFEMBJ7593372_CR17","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/0076-6879(95)62007-9","volume":"262","author":"JM L\u00e1zaro","year":"1995","unstructured":"L\u00e1zaro JM, Blanco L and Salas M (1995) Purification of bacteriophage \u03c629 DNA polymerase. Methods Enzymol, 262, 42\u201349.","journal-title":"Methods Enzymol"},{"key":"BFEMBJ7593372_CR18","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1126\/science.282.5393.1516","volume":"282","author":"KP Lemon","year":"1998","unstructured":"Lemon KP and Grossman AD (1998) Localization of bacterial DNA polymerase: evidence for a factory model of replication. Science, 282, 1516\u20131519.","journal-title":"Science"},{"key":"BFEMBJ7593372_CR19","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1093\/emboj\/19.4.710","volume":"19","author":"PJ Lewis","year":"2000","unstructured":"Lewis PJ, Thaker SD and Errington J (2000) Compartmentalization of transcription and translation in Bacillus subtilis. EMBO J, 19, 710\u2013718.","journal-title":"EMBO J"},{"key":"BFEMBJ7593372_CR20","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/BF00436183","volume":"196","author":"K Matsumoto","year":"1984","unstructured":"Matsumoto K, Saito T, Kim CI, Ando T and Hirokawa H (1984) Bacteriophage \u03c629 DNA replication in vitro: participation of the terminal protein and the gene 2 product in elongation. Mol Gen Genet, 196, 381\u2013386.","journal-title":"Mol Gen Genet"},{"key":"BFEMBJ7593372_CR21","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/0147-619X(86)90046-6","volume":"15","author":"T McKenzie","year":"1986","unstructured":"McKenzie T, Hoshino T, Tanaka T and Sueoka N (1986) The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid, 15, 93\u2013103.","journal-title":"Plasmid"},{"key":"BFEMBJ7593372_CR22","doi-asserted-by":"crossref","first-page":"4182","DOI":"10.1093\/emboj\/19.15.4182","volume":"19","author":"WJJ Meijer","year":"2000","unstructured":"Meijer WJJ, Lewis PJ, Errington J and Salas M (2000) Dynamic relocalization of phage \u03c629 DNA during its replication cycle in Bacillus subtilis and the role of the viral protein p16.7. EMBO J, 19, 4182\u20134190.","journal-title":"EMBO J"},{"key":"BFEMBJ7593372_CR23","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1128\/jvi.19.2.495-500.1976","volume":"19","author":"RP Mellado","year":"1976","unstructured":"Mellado RP, Moreno F, Vi\u00f1uela E, Salas M, Reilly BE and Anderson DL (1976) Genetic analysis of bacteriophage \u03c629 of Bacillus subtilis phage \u03c629: integration and mapping of reference mutants of two collections. J Virol, 19, 495\u2013500.","journal-title":"J Virol"},{"key":"BFEMBJ7593372_CR24","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/0042-6822(80)90367-0","volume":"104","author":"RP Mellado","year":"1980","unstructured":"Mellado RP, Pe\u00f1alva MA, Inciarte MR and Salas M (1980) The protein covalently linked to the 5\u2032 termini of the DNA of Bacillus subtilis phage \u03c629 is involved in the initiation of DNA replication. Virology, 104, 84\u201396.","journal-title":"Virology"},{"key":"BFEMBJ7593372_CR25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0042-6822(74)90298-0","volume":"62","author":"F Moreno","year":"1974","unstructured":"Moreno F, Camacho A, Vi\u00f1uela E and Salas M (1974) Suppressor\u2010sensitive mutants and genetic map of Bacillus subtilis bacteriophage \u03c629. Virology, 62, 1\u201316.","journal-title":"Virology"},{"key":"BFEMBJ7593372_CR26","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/0003-2697(81)90783-1","volume":"117","author":"JH Morrissey","year":"1981","unstructured":"Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem, 117, 307\u2013310.","journal-title":"Anal Biochem"},{"key":"BFEMBJ7593372_CR27","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S0955-0674(96)80011-1","volume":"8","author":"J Newport","year":"1996","unstructured":"Newport J and Yan H (1996) Organization of DNA into foci during replication. Curr Opin Cell Biol, 8, 365\u2013368.","journal-title":"Curr Opin Cell Biol"},{"key":"BFEMBJ7593372_CR28","doi-asserted-by":"crossref","first-page":"5075","DOI":"10.1002\/j.1460-2075.1994.tb06837.x","volume":"13","author":"A Pombo","year":"1994","unstructured":"Pombo A, Ferreira J, Bridge E and Carmo\u2010Fonseca M (1994) Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells. EMBO J, 13, 5075\u20135085.","journal-title":"EMBO J"},{"key":"BFEMBJ7593372_CR29","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev.bi.60.070191.000351","volume":"60","author":"M Salas","year":"1991","unstructured":"Salas M (1991) Protein\u2010priming of DNA replication. Annu Rev Biochem, 60, 39\u201371.","journal-title":"Annu Rev Biochem"},{"key":"BFEMBJ7593372_CR30","first-page":"843","volume-title":"Bacillus subtilis and other Gram\u2010positive Bacteria: Biochemistry, Physiology, and Molecular Genetics","author":"M Salas","year":"1993","unstructured":"Salas M and Rojo F (1993) Replication and transcription of bacteriophage \u03c629 DNA. In Sonenshein,A.L., Hoch,J.A. and Losick,R. (eds), Bacillus subtilis and other Gram\u2010positive Bacteria: Biochemistry, Physiology, and Molecular Genetics. American Society for Microbiology, Washington, DC, pp. 843\u2013857."},{"key":"BFEMBJ7593372_CR31","first-page":"131","volume-title":"DNA Replication in Eukaryotic Cells","author":"M Salas","year":"1996","unstructured":"Salas M, Miller JT, Leis J and DePamphilis ML (1996) Mechanisms for priming DNA synthesis. In DePamphilis,M.L. (ed.), DNA Replication in Eukaryotic Cells. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 131\u2013176."},{"key":"BFEMBJ7593372_CR32","volume-title":"Molecular Cloning: A Laboratory Manual","author":"J Sambrook","year":"1989","unstructured":"Sambrook J, Fritsch EF and Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.","edition":"2nd"},{"key":"BFEMBJ7593372_CR33","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","volume":"74","author":"F Sanger","year":"1977","unstructured":"Sanger F, Nicklen S and Coulson AR (1977) DNA sequencing with chain\u2010terminating inhibitors. Proc Natl Acad Sci USA, 74, 5463\u20135467.","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFEMBJ7593372_CR34","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/0003-2697(87)90587-2","volume":"166","author":"H Schagger","year":"1987","unstructured":"Schagger H and Jagow G (1987) Tricine\u2013sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem, 166, 368\u2013379.","journal-title":"Anal Biochem"},{"key":"BFEMBJ7593372_CR35","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1006\/jmbi.1994.1364","volume":"239","author":"MS Soengas","year":"1994","unstructured":"Soengas MS, Esteban JA, Salas M and Guti\u00e9rrez C (1994) Complex formation between phage \u03c629 single\u2010stranded DNA binding protein and DNA. J Mol Biol, 239, 213\u2013226.","journal-title":"J Mol Biol"},{"key":"BFEMBJ7593372_CR36","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1093\/nar\/12.4.1943","volume":"12","author":"JM Sogo","year":"1984","unstructured":"Sogo JM, Lozano M and Salas M (1984) In vitro transcription of the Bacillus subtilis phage \u03c629 DNA by Bacillus subtilis and Escherichia coli RNA polymerases. Nucleic Acids Res, 12, 1943\u20131960.","journal-title":"Nucleic Acids Res"},{"key":"BFEMBJ7593372_CR37","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/0042-6822(71)90062-6","volume":"46","author":"A Talavera","year":"1971","unstructured":"Talavera A, Jim\u00e9nez F, Salas M and Vi\u00f1uela E (1971) Temperature\u2010sensitive mutants of bacteriophage \u03c629. Virology, 46, 586\u2013595.","journal-title":"Virology"},{"key":"BFEMBJ7593372_CR38","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/0378-1119(82)90149-4","volume":"17","author":"H Yoshikawa","year":"1982","unstructured":"Yoshikawa H and Ito J (1982) Nucleotide sequence of the major early region of bacteriophage \u03c629. Gene, 17, 323\u2013335.","journal-title":"Gene"},{"key":"BFEMBJ7593372_CR39","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/0378-1119(89)90104-2","volume":"83","author":"A Zaballos","year":"1989","unstructured":"Zaballos A, L\u00e1zaro JM, M\u00e9ndez E, Mellado RP and Salas M (1989) Effects of internal deletions on the priming activity of the phage \u03c629 terminal protein. Gene, 83, 187\u2013195.","journal-title":"Gene"}],"container-title":["The EMBO Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1093%2Femboj%2F19.20.5575","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1093\/emboj\/19.20.5575.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1093\/emboj\/19.20.5575\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1093\/emboj\/19.20.5575","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1093\/emboj\/19.20.5575.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T18:37:56Z","timestamp":1779302276000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1093\/emboj\/19.20.5575"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10,16]]},"references-count":39,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2000,10,16]]}},"alternative-id":["BFEMBJ7593372"],"URL":"https:\/\/doi.org\/10.1093\/emboj\/19.20.5575","relation":{},"ISSN":["0261-4189","1460-2075"],"issn-type":[{"value":"0261-4189","type":"print"},{"value":"1460-2075","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10,16]]},"assertion":[{"value":"10 July 2000","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 August 2000","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 August 2000","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 October 2000","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}