{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T04:37:25Z","timestamp":1791693445938,"version":"4.3.4"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"9","funder":[{"DOI":"10.13039\/100000139","name":"Environmental Protection Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000139","id-type":"DOI","asserted-by":"publisher"}]},{"name":"C. Gus Glasscock, Jr. Endowed Fund for Excellence in Environmental Sciences"},{"DOI":"10.13039\/100007492","name":"Baylor University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The glucocorticoid receptor (GR) is present in almost every vertebrate cell and is utilized in many biological processes. Despite an abundance of mammalian data, the structural conservation of the receptor and cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and\/or replace animal testing have increased, driving the impetus to advance development of new approach methodologies (NAMs). Here we used in silico, in vitro, and in vivo methods to elucidate a greater understanding of receptor-mediated effects of synthetic glucocorticoid exposure in teleost fish. Evolutionary conservation of amino acid residues critical for transcriptional activation was confirmed in silico using sequence alignment to predict across species susceptibility. Subsequent in vitro assays using zebrafish and human GR provided evidence of physiological congruence of GR agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed in vivo to the synthetic glucocorticoids, dexamethasone (0.04, 400, 4,000\u2009\u00b5g\/L) and beclomethasone dipropionate (130\u2009\u00b5g\/L), and GR agonism confirmed via digital polymerase chain reaction; in addition, EcoToxChip analyses identified potential mRNA biomarkers following glucocorticoid exposure. These findings support the use of NAMs to potentially reduce multispecies in vivo experimentation while providing empirical evidence that expands the taxonomic domain of applicability for the GR agonism molecular initiating event within the broader GR agonism adverse outcome pathway network.<\/jats:p>","DOI":"10.1093\/etojnl\/vgae041","type":"journal-article","created":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T04:31:36Z","timestamp":1736137896000},"page":"2545-2556","source":"Crossref","is-referenced-by-count":3,"title":["Comparative glucocorticoid receptor agonism: In silico, in vitro, and in vivo and identification of potential biomarkers for synthetic glucocorticoid exposure"],"prefix":"10.1093","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4608-2518","authenticated-orcid":false,"given":"Alexander R","family":"Cole","sequence":"first","affiliation":[{"name":"Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University , Waco, TX,","place":["United States"]},{"name":"Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1296-4539","authenticated-orcid":false,"given":"Brett R","family":"Blackwell","sequence":"additional","affiliation":[{"name":"Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7883-4740","authenticated-orcid":false,"given":"Jenna E","family":"Cavallin","sequence":"additional","affiliation":[{"name":"Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9646-2300","authenticated-orcid":false,"given":"Jacob E","family":"Collins","sequence":"additional","affiliation":[{"name":"Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Great Lakes Toxicology and Ecology Division , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley R","family":"Kittelson","sequence":"additional","affiliation":[{"name":"Oak Ridge Institute for Science and Education, US Environmental Protection Agency, Great Lakes Toxicology and Ecology Division , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yesmeena M","family":"Shmaitelly","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University , Waco, TX,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2095-7163","authenticated-orcid":false,"given":"Laura M","family":"Langan","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University , Waco, TX,","place":["United States"]},{"name":"Department of Environmental Health Sciences, University of South Carolina , Columbia, SC,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2801-0203","authenticated-orcid":false,"given":"Daniel L","family":"Villeneuve","sequence":"additional","affiliation":[{"name":"Great Lakes Toxicology and Ecology Division, US Environmental Protection Agency , Duluth, MN,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6277-9852","authenticated-orcid":false,"given":"Bryan W","family":"Brooks","sequence":"additional","affiliation":[{"name":"Department of Environmental Science, Center for Reservoir and Aquatic Systems Research, Baylor University , Waco, TX,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,1,6]]},"reference":[{"key":"2025082902521405800_vgae041-B1","doi-asserted-by":"publisher","first-page":"1048008","DOI":"10.3389\/fphys.2022.1048008","article-title":"Role of glucocorticoid and mineralocorticoid receptors in rainbow trout (Oncorhynchus mykiss) skeletal muscle: A transcriptomic perspective of cortisol action","volume":"13","author":"Aedo","year":"2023","journal-title":"Frontiers in Physiology"},{"key":"2025082902521405800_vgae041-B7207168","doi-asserted-by":"publisher","first-page":"5024","DOI":"10.1021\/acs.est.0c05942","article-title":"Development of a Comprehensive Toxicity Pathway Model for 17\u03b1-Ethinylestradiol in Early Life Stage Fathead Minnows (","volume":"55","author":"Alcaraz","year":"2021","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B2","doi-asserted-by":"publisher","first-page":"2692","DOI":"10.1210\/jc.2009-0370","article-title":"The pathogenetic role of cortisol in the metabolic syndrome: A hypothesis","volume":"94","author":"Anagnostis","year":"2009","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2025082902521405800_vgae041-B3","doi-asserted-by":"publisher","first-page":"109697","DOI":"10.1016\/j.cbpc.2023.109697","article-title":"Apical and mechanistic effects of 6PPD-quinone on different life-stages of the fathead minnow (Pimephales promelas)","volume":"271","author":"Anderson-Bain","year":"2023","journal-title":"Comparative Biochemistry and Physiology. Toxicology & Pharmacology"},{"key":"2025082902521405800_vgae041-B4","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1002\/etc.34","article-title":"Adverse outcome pathways: A conceptual framework to support ecotoxicology research and risk assessment","volume":"29","author":"Ankley","year":"2010","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B5","doi-asserted-by":"publisher","first-page":"8211","DOI":"10.1021\/es072658j","article-title":"Repeating history: Pharmaceuticals in the environment","volume":"41","author":"Ankley","year":"2007","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B6","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.jsbmb.2013.07.009","article-title":"Evolution of hormone selectivity in glucocorticoid and mineralocorticoid receptors","volume":"137","author":"Baker","year":"2013","journal-title":"Journal of Steroid Biochemistry and Molecular Biology"},{"key":"2025082902521405800_vgae041-B7","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1186\/s12866-020-01848-x","article-title":"Identification of glucocorticoid receptor in Drosophila melanogaster","volume":"20","author":"Bartolo","year":"2020","journal-title":"BMC Microbiology"},{"key":"2025082902521405800_vgae041-B8","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1002\/etc.4309","article-title":"EcoToxChip: A next-generation toxicogenomics tool for chemical prioritization and environmental management","volume":"38","author":"Basu","year":"2019","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B9","doi-asserted-by":"publisher","first-page":"463A","DOI":"10.1021\/es040675s","article-title":"Peer reviewed: Meeting the scientific needs of ecological risk assessment in a regulatory context","volume":"38","author":"Bradbury","year":"2004","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B10","doi-asserted-by":"publisher","first-page":"2575","DOI":"10.1039\/c8gc03893g","article-title":"Greening chemistry and ecotoxicology towards sustainable environmental quality","volume":"21","author":"Brooks","year":"2019","journal-title":"Green Chemistry"},{"key":"2025082902521405800_vgae041-B11","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1002\/etc.5754","article-title":"Towards precision ecotoxicology: Leveraging evolutionary conservation of pharmaceutical and personal care product targets to understand adverse outcomes across species and life stages","volume":"43","author":"Brooks","year":"2024","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B12","doi-asserted-by":"publisher","first-page":"1796","DOI":"10.4049\/jimmunol.1300087","article-title":"Serum and glucocorticoid\u2013regulated kinase 1 regulates neutrophil clearance during inflammation resolution","volume":"192","author":"Burgon","year":"2014","journal-title":"Journal of Immunology"},{"key":"2025082902521405800_vgae041-B13","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.ygcen.2007.03.009","article-title":"Evolution of the corticosteroid receptor signalling pathway in fish","volume":"153","author":"Bury","year":"2007","journal-title":"General and Comparative Endocrinology"},{"key":"2025082902521405800_vgae041-B14","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1677\/jme.0.0310141","article-title":"Evidence for two distinct functional glucocorticoid receptors in teleost fish","volume":"31","author":"Bury","year":"2003","journal-title":"Journal of Molecular Endocrinology"},{"key":"2025082902521405800_vgae041-B8300510","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1177\/1535370217750088","article-title":"Biomarker definitions and their applications","volume":"243","author":"Califf","year":"2018","journal-title":"Experimental Biology and Medicine"},{"key":"2025082902521405800_vgae041-B15","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1021\/acs.est.0c05269","article-title":"Effects-based monitoring of bioactive chemicals discharged to the Colorado River before and after a municipal wastewater treatment plant replacement","volume":"55","author":"Cavallin","year":"2021","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B16","doi-asserted-by":"publisher","first-page":"117928","DOI":"10.1016\/j.envpol.2021.117928","article-title":"Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA","volume":"289","author":"Cavallin","year":"2021","journal-title":"Environmental Pollution"},{"key":"2025082902521405800_vgae041-B81026613","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.mce.2008.09.031","article-title":"The role and regulation of 11beta-hydroxysteroid dehydrogenase type 1 in the inflammatory response","volume":"301","author":"Chapman","year":"2009","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2025082902521405800_vgae041-B7921037","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1007\/s11745-004-1329-9","article-title":"Structure and function of animal fatty acid synthase","volume":"39","author":"Chirala","year":"2004","journal-title":"Lipids"},{"key":"2025082902521405800_vgae041-B7384227","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1681\/ASN.2021050723","article-title":"DGAT2 Inhibition Potentiates Lipid Droplet Formation To Reduce Cytotoxicity in APOL1 Kidney Risk Variants","volume":"33","author":"Chun","year":"2022","journal-title":"Journal of the American Society of Nephrology"},{"key":"2025082902521405800_vgae041-B17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5599\/admet.1513","article-title":"Role of in vitro two-dimensional (2D) and three-dimensional (3D) cell culture systems for ADME-Tox screening in drug discovery and development: A comprehensive review","volume":"11","author":"Chunduri","year":"2022","journal-title":"Admet & Dmpk"},{"key":"2025082902521405800_vgae041-B18","doi-asserted-by":"publisher","first-page":"121638","DOI":"10.1016\/j.envpol.2023.121638","article-title":"Global occurrence of synthetic glucocorticoids and glucocorticoid receptor agonistic activity, and aquatic hazards in effluent discharges and freshwater systems","volume":"329","author":"Cole","year":"2023","journal-title":"Environmental Pollution"},{"key":"2025082902521405800_vgae041-B19","doi-asserted-by":"publisher","first-page":"3082","DOI":"10.1021\/acsestwater.3c00253","article-title":"Comparative endpoint sensitivity of bioanalytical tools for glucocorticoid receptor agonism surveillance in aquatic matrices","volume":"3","author":"Cole","year":"2023","journal-title":"ACS ES&T Water"},{"key":"2025082902521405800_vgae041-B21","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.chemosphere.2018.07.190","article-title":"Downstream trends of in vitro bioassay responses in a wastewater effluent-dominated River","volume":"212","author":"Daniels","year":"2018","journal-title":"Chemosphere"},{"key":"2025082902521405800_vgae041-B22","doi-asserted-by":"publisher","first-page":"4376","DOI":"10.1021\/acs.est.9b06607","article-title":"EcoToxModules: Custom gene sets to organize and analyze toxicogenomics data from ecological species","volume":"54","author":"Ewald","year":"2020","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B9054614","doi-asserted-by":"publisher","first-page":"114387","DOI":"10.1016\/j.ygcen.2023.114387","article-title":"Molecular mechanisms of the stress-induced regulation of the inflammatory response in fish","volume":"345","author":"Faught","year":"2024","journal-title":"General and Comparative Endocrinology"},{"key":"2025082902521405800_vgae041-B23","first-page":"523","article-title":"The welfare of animals used in science: How the \u201cThree Rs\u201d ethic guides improvements","volume":"50","author":"Fenwick","year":"2009","journal-title":"The Canadian Veterinary Journal = La Revue Veterinaire Canadienne"},{"key":"2025082902521405800_vgae041-B5827433","doi-asserted-by":"publisher","first-page":"120614","DOI":"10.1016\/j.lfs.2022.120614","article-title":"Differential expression of genes regulated by the glucocorticoid receptor pathway in patients with pulmonary tuberculosis","volume":"301","author":"Gallucci","year":"2022","journal-title":"Life Sciences"},{"key":"2025082902521405800_vgae041-B24","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1016\/j.toxrep.2019.05.013","article-title":"Glucocorticoid receptor-dependent induction of cripto-1 (one-eyed pinhead) inhibits zebrafish caudal fin regeneration","volume":"6","author":"Garland","year":"2019","journal-title":"Toxicology Reports"},{"key":"2025082902521405800_vgae041-B25","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1210\/en.2004-1390","article-title":"Mineralocorticoid receptors and hormones: Fishing for answers","volume":"146","author":"Gilmour","year":"2005","journal-title":"Endocrinology"},{"key":"2025082902521405800_vgae041-B26","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1038\/nmeth.2633","article-title":"Absolute quantification by droplet digital PCR versus analog real-time PCR","volume":"10","author":"Hindson","year":"2013","journal-title":"Nature Methods"},{"key":"2025082902521405800_vgae041-B130837","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1036844","article-title":"Serum and glucocorticoid-regulated kinase 1: Structure, biological functions, and its inhibitors","volume":"13","author":"Jang","year":"2022","journal-title":"Frontiers in Pharmacology"},{"key":"2025082902521405800_vgae041-B27","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1002\/etc.5501","article-title":"Defining the biologically plausible taxonomic domain of applicability of an adverse outcome pathway: A case study linking nicotinic acetylcholine receptor activation to colony death","volume":"42","author":"Jensen","year":"2023","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B8503546","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.cbpa.2019.01.010","article-title":"What can we learn from glucocorticoid administration in fish? Effects of cortisol and dexamethasone on intermediary metabolism of gilthead seabream (Sparus aurata L.)","volume":"231","author":"Jerez-Cepa","year":"2019","journal-title":"Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology"},{"key":"2025082902521405800_vgae041-B6413641","doi-asserted-by":"publisher","first-page":"734","DOI":"10.1016\/j.bbrc.2018.09.023","article-title":"ODC1 inhibits the inflammatory response and ROS-induced apoptosis in macrophages","volume":"504","author":"Jiang","year":"2018","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2025082902521405800_vgae041-B28","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1042\/cs1010739","article-title":"Cortisol increases gluconeogenesis in humans: Its role in the metabolic syndrome","volume":"101","author":"Khani","year":"2001","journal-title":"Clinical Science"},{"key":"2025082902521405800_vgae041-B29","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1007\/s10646-006-0111-3","article-title":"Sex steroid receptor evolution and signalling in aquatic invertebrates","volume":"16","author":"K\u00f6hler","year":"2007","journal-title":"Ecotoxicology"},{"key":"2025082902521405800_vgae041-B30","doi-asserted-by":"publisher","first-page":"2377","DOI":"10.1021\/es104105e","article-title":"Synthetic glucocorticoids in the environment: First results on their potential impacts on fish","volume":"45","author":"Kugathas","year":"2011","journal-title":"Environmental Science & Technology"},{"key":"2025082902521405800_vgae041-B31","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1093\/toxsci\/kfw119","article-title":"Editor\u2019s highlight: Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS): A web-based tool for addressing the challenges of cross-species extrapolation of chemical toxicity","volume":"153","author":"LaLone","year":"2016","journal-title":"Toxicological Sciences"},{"key":"2025082902521405800_vgae041-B2322207","doi-asserted-by":"publisher","first-page":"1","DOI":"10.15698\/cst2019.01.170","article-title":"Serum- and glucocorticoid-inducible kinase 1 and the response to cell stress","volume":"3","author":"Lang","year":"2018","journal-title":"Cell Stress"},{"key":"2025082902521405800_vgae041-B6411135","doi-asserted-by":"publisher","first-page":"115570","DOI":"10.1016\/j.ecoenv.2023.115570","article-title":"Transcriptomic profiling of TLR-7-mediated immune-challenge in zebrafish embryos in the presence and absence of glucocorticoid-induced immunosuppression","volume":"266","author":"Luckner","year":"2023","journal-title":"Ecotoxicology and Environmental Safety"},{"key":"2025082902521405800_vgae041-B32","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1159\/000321244","volume-title":"Endocrine development","author":"Lui","year":"2011"},{"key":"2025082902521405800_vgae041-B270899976","doi-asserted-by":"publisher","first-page":"e13316","DOI":"10.1016\/j.heliyon.2023.e13316","article-title":"Role of ACSL5 in fatty acid metabolism","volume":"9","author":"Luo","year":"2023","journal-title":"Heliyon"},{"key":"2025082902521405800_vgae041-B33","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.1002\/ieam.4725","article-title":"A framework for prioritizing contaminants in retrospective ecological assessments: Application in the Milwaukee Estuary (Milwaukee, WI)","volume":"19","author":"Maloney","year":"2023","journal-title":"Integrated Environmental Assessment and Management"},{"key":"2025082902521405800_vgae041-B34","doi-asserted-by":"publisher","first-page":"1229","DOI":"10.1002\/etc.5571","article-title":"Evaluation of complex mixture toxicity in the Milwaukee Estuary (WI, USA) using whole-mixture and component-based evaluation methods","volume":"42","author":"Maloney","year":"2023","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B8254141","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1080\/15287390490428198","article-title":"Biomarkers of exposure in community settings","volume":"67","author":"Metcalf","year":"2004","journal-title":"Journal of Toxicology and Environmental Health. Part A"},{"key":"2025082902521405800_vgae041-B35","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.devcel.2019.11.016","article-title":"NF-\u039aB signaling negatively regulates osteoblast dedifferentiation during zebrafish bone regeneration","volume":"52","author":"Mishra","year":"2020","journal-title":"Developmental Cell"},{"key":"2025082902521405800_vgae041-B20","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council, Committee for the Update of the Guide for the Care, Institute for Laboratory Animal Research, Division on Earth and Life Studies","year":"2010","edition":"8th ed.)"},{"key":"2025082902521405800_vgae041-B36","doi-asserted-by":"publisher","first-page":"e29462","DOI":"10.1371\/journal.pone.0029462","article-title":"Morpholino gene knockdown in adult Fundulus heteroclitus: Role of SGK1 in seawater acclimation","volume":"6","author":"Notch","year":"2011","journal-title":"PloS One"},{"key":"2025082902521405800_vgae041-B37","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1016\/j.jaci.2013.09.007","article-title":"The biology of the glucocorticoid receptor: New signaling mechanisms in health and disease","volume":"132","author":"Oakley","year":"2013","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"2025082902521405800_vgae041-B68068528","doi-asserted-by":"publisher","first-page":"E28","DOI":"10.1152\/ajpendo.90897.2008","article-title":"Biochemical and physiological function of stearoyl-CoA desaturase","volume":"297","author":"Paton","year":"2009","journal-title":"American Journal of Physiology. Endocrinology and Metabolism"},{"key":"2025082902521405800_vgae041-B38","doi-asserted-by":"publisher","first-page":"1591","DOI":"10.1210\/en.2007-1364","article-title":"Discovery of a functional glucocorticoid receptor beta-isoform in zebrafish","volume":"149","author":"Schaaf","year":"2008","journal-title":"Endocrinology"},{"key":"2025082902521405800_vgae041-B39","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/j.chemosphere.2013.04.091","article-title":"Occurrence of glucocorticogenic activity in various surface waters in the Netherlands","volume":"93","author":"Schriks","year":"2013","journal-title":"Chemosphere"},{"key":"2025082902521405800_vgae041-B40","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/j.cbpc.2012.05.003","article-title":"Alternate glucocorticoid receptor ligand binding structures influence outcomes in an in vivo tissue regeneration model","volume":"156","author":"Sengupta","year":"2012","journal-title":"Comparative Biochemistry and Physiology. Toxicology & Pharmacology"},{"key":"2025082902521405800_vgae041-B41","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Molecular Systems Biology"},{"key":"2025082902521405800_vgae041-B9975817","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.cbpa.2017.11.011","article-title":"Characterization of two channel catfish, Ictalurus punctatus, glucocorticoid receptors and expression following an acute stressor","volume":"216","author":"Small","year":"2018","journal-title":"Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology"},{"key":"2025082902521405800_vgae041-B42","author":"Society for the Advancement of AOPs","year":"2024"},{"key":"2025082902521405800_vgae041-B43","doi-asserted-by":"publisher","first-page":"e7975","DOI":"10.7717\/peerj.7975","article-title":"T1000: A reduced gene set prioritized for toxicogenomic studies","volume":"7","author":"Soufan","year":"2019","journal-title":"PeerJ"},{"key":"2025082902521405800_vgae041-B44","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1002\/etc.5251","article-title":"EcoToxXplorer: Leveraging design thinking to develop a standardized web-based transcriptomics analytics platform for diverse users","volume":"41","author":"Soufan","year":"2022","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2025082902521405800_vgae041-B62850188","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1677\/joe.1.06703","article-title":"Evolution of glucocorticoid receptors with different glucocorticoid sensitivity","volume":"190","author":"Stolte","year":"2006","journal-title":"Journal of Endocrinology"},{"key":"2025082902521405800_vgae041-B45","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1186\/s12864-022-08831-y","article-title":"Reducing costs for DNA and RNA sequencing by sample pooling using a metagenomic approach","volume":"23","author":"Teufel","year":"2022","journal-title":"BMC Genomics"},{"key":"2025082902521405800_vgae041-B14076825","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1097\/MCO.0000000000000031","article-title":"Fatty acid binding proteins: tissue-specific functions in health and disease","volume":"17","author":"Thumser","year":"2014","journal-title":"Current Opinion in Clinical Nutrition and Metabolic Care"},{"key":"2025082902521405800_vgae041-B46","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nrm.2016.152","article-title":"Glucocorticoid receptor control of transcription: Precision and plasticity via allostery","volume":"18","author":"Weikum","year":"2017","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"2025082902521405800_vgae041-B1067856","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1177\/108705719900400206","article-title":"A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays","volume":"4","author":"Zhang","year":"1999","journal-title":"Journal of Biomolecular Screening"},{"key":"2025082902521405800_vgae041-B47","doi-asserted-by":"publisher","first-page":"9582","DOI":"10.3390\/ijms23179582","article-title":"Histone deacetylases cooperate with NF-\u039aB to support the immediate migratory response after zebrafish pronephros injury","volume":"23","author":"Zhuang","year":"2022","journal-title":"International Journal of Molecular Sciences"}],"container-title":["Environmental Toxicology and Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/etc\/advance-article-pdf\/doi\/10.1093\/etojnl\/vgae041\/61339991\/vgae041.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/44\/9\/2545\/61339991\/vgae041.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/44\/9\/2545\/61339991\/vgae041.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T06:52:18Z","timestamp":1756450338000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/etc\/article\/44\/9\/2545\/7942960"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,6]]},"references-count":66,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,1,6]]},"published-print":{"date-parts":[[2025,9,1]]}},"URL":"https:\/\/doi.org\/10.1093\/etojnl\/vgae041","relation":{},"ISSN":["0730-7268","1552-8618"],"issn-type":[{"value":"0730-7268","type":"print"},{"value":"1552-8618","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,9]]},"published":{"date-parts":[[2025,1,6]]}}}