{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:59:22Z","timestamp":1790359162129,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Environmental contaminants frequently occur as complex mixtures in agricultural systems, yet their biological effects may persist even when residues are no longer detectable in harvested soybean seeds. This study evaluated whether a single soil exposure to ciprofloxacin (CIP), applied alone or in combination with glyphosate (G), induces persistent metabolic and developmental responses in soybean (Glycine max). Soybean plants were exposed at sowing to environmentally relevant concentrations of CIP (0.10 and 1.00\u2009mg L\u2212\u00b9), either alone or combined with G. Ciprofloxacin residues were not detected in harvested seeds. However, integrated ultra-high-performance liquid chromatography, gas chromatography\u2013flame ionization detector, and ^1H nuclear magnetic resonance analyses revealed treatment-dependent alterations in folic acid, isoflavones, carbohydrate-related metabolites, and selected fatty acids. Exploratory multivariate analyses revealed distinct treatment-associated patterns and identified the low-dose combined exposure treatment as the most distinct metabolic profile. Although germination remained unaffected, progeny seedlings exhibited treatment-dependent differences in primary root development, indicating carry-over effects associated with parental exposure. The observed responses were particularly evident under combined exposure conditions, suggesting metabolic modulation associated with combined exposure. Collectively, these findings indicate that early-life contaminant exposure following a single application at sowing can be associated with persistent biochemical and developmental responses. These results highlight the importance of integrating metabolomic approaches with conventional residue analysis to improve the assessment of biological responses to emerging contaminants and contaminant mixtures in agroecosystems.<\/jats:p>","DOI":"10.1093\/etojnl\/vgag238","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:16:29Z","timestamp":1788297389000},"source":"Crossref","is-referenced-by-count":0,"title":["Early-life ciprofloxacin exposure induces carry-over metabolic and developmental responses in soybean under single and combined glyphosate exposure"],"prefix":"10.1093","author":[{"given":"Sof\u00eda A","family":"Gegenschatz","sequence":"first","affiliation":[{"name":"Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ), C\u00e1tedra de Qu\u00edmica Anal\u00edtica I, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Ciudad Universitaria , Santa Fe,","place":["Argentina"]},{"name":"C\u00e1tedra de Qu\u00edmica Org\u00e1nica y Biol\u00f3gica, Facultad de Ciencias Agropecuarias, Universidad Nacional de Entre R\u00edos , Oro verde,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celina M","family":"Monz\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Control de F\u00e1rmacos y T\u00f3xicos, CONICET-IQUIBA NEA, Facultad de Ciencias Exactas Naturales y Agrimensura, Universidad Nacional del Nordeste , Corrientes,","place":["Argentina"]},{"name":"Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9s","family":"Charris-Molina","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) , Ciudad Aut\u00f3noma de Buenos Aires,","place":["Argentina"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian J","family":"Sequin","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Qu\u00edmica Org\u00e1nica y Biol\u00f3gica, Facultad de Ciencias Agropecuarias, Universidad Nacional de Entre R\u00edos , Oro verde,","place":["Argentina"]},{"name":"Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo A","family":"Hoijemberg","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) , Ciudad Aut\u00f3noma de Buenos Aires,","place":["Argentina"]},{"name":"Escuela de Ciencia y Tecnologia (ECyT) - Universidad Nacional de San Mart\u00edn (UNSAM), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9ctor C","family":"Goicoechea","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ), C\u00e1tedra de Qu\u00edmica Anal\u00edtica I, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Ciudad Universitaria , Santa Fe,","place":["Argentina"]},{"name":"Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla M","family":"Teglia","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ), C\u00e1tedra de Qu\u00edmica Anal\u00edtica I, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Ciudad Universitaria , Santa Fe,","place":["Argentina"]},{"name":"Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana A","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Laboratorio de Desarrollo Anal\u00edtico y Quimiometr\u00eda (LADAQ), C\u00e1tedra de Qu\u00edmica Anal\u00edtica I, Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Ciudad Universitaria , Santa Fe,","place":["Argentina"]},{"name":"Facultad de Bioqu\u00edmica y Ciencias Biol\u00f3gicas, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"2026092513145772900_vgag238-B1","doi-asserted-by":"publisher","first-page":"5987","DOI":"10.1002\/ps.70742","article-title":"Thirty years of glyphosate-resistant crops and weeds: Current situation and future prospects","volume":"82","author":"Alc\u00e1ntara-de la Cruz","year":"2026","journal-title":"Pest Management Science"},{"key":"2026092513145772900_vgag238-B2","doi-asserted-by":"publisher","first-page":"8697","DOI":"10.1021\/ac3019284","article-title":"Sensitivity equation for quantitative analysis with multivariate curve resolution-alternating least-squares: Theoretical and experimental approach","volume":"84","author":"Bauza","year":"2012","journal-title":"Analytical Chemistry"},{"key":"2026092513145772900_vgag238-B3","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1002\/jeq2.20655","article-title":"Effects of glyphosate on antibiotic resistance in soil bacteria and its potential significance: A review","volume":"54","author":"Bearson","year":"2025","journal-title":"Journal of Environmental Quality"},{"key":"2026092513145772900_vgag238-B4","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1139\/o59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Canadian Journal of Biochemistry and Physiology"},{"key":"2026092513145772900_vgag238-B5","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1007\/s12079-011-0119-1","article-title":"Hormesis provides a generalized quantitative estimate of biological plasticity","volume":"5","author":"Calabrese","year":"2011","journal-title":"Journal of Cell Communication and Signaling"},{"key":"2026092513145772900_vgag238-B6","doi-asserted-by":"publisher","first-page":"e96580","DOI":"10.1371\/journal.pone.0096580","article-title":"Quantifying synergy: A systematic review of mixture toxicity studies within environmental toxicology","volume":"9","author":"Cedergreen","year":"2014","journal-title":"PLoS One"},{"key":"2026092513145772900_vgag238-B7","doi-asserted-by":"publisher","first-page":"175487","DOI":"10.1016\/j.scitotenv.2024.175487","article-title":"Fate of emerging antibiotics in soil-plant systems: A case on fluoroquinolones","volume":"951","author":"Chen","year":"2024","journal-title":"Science of the Total Environment"},{"key":"2026092513145772900_vgag238-B8","doi-asserted-by":"publisher","first-page":"1282","DOI":"10.1021\/ac048630x","article-title":"Statistical total correlation spectroscopy: An exploratory approach for latent biomarker identification from metabolic 1H NMR data sets","volume":"77","author":"Cloarec","year":"2005","journal-title":"Analytical Chemistry"},{"key":"2026092513145772900_vgag238-B9","doi-asserted-by":"publisher","first-page":"e25689","DOI":"10.1002\/anie.202525689","article-title":"Recent trends in metabolomics by NMR spectroscopy","volume":"65","author":"Di Paco","year":"2026","journal-title":"Angewandte Chemie (International ed. in English)"},{"key":"2026092513145772900_vgag238-B10","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.csbj.2016.02.005","article-title":"A guide to the identification of metabolites in NMR-based metabonomics\/metabolomics experiments","volume":"14","author":"Dona","year":"2016","journal-title":"Computational and Structural Biotechnology Journal"},{"key":"2026092513145772900_vgag238-B11","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/j.envpol.2019.03.026","article-title":"Enrofloxacin and Roundup\u00ae interactive effects on the aquatic macrophyte Elodea canadensis physiology","volume":"249","author":"Gomes","year":"2019","journal-title":"Environmental Pollution"},{"key":"2026092513145772900_vgag238-B12","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-2220-0_8","volume-title":"Crop stress and its management: Perspectives and strategies","author":"Hasanuzzaman","year":"2012"},{"key":"2026092513145772900_vgag238-B13","author":"International Organization for Standardization","year":"2000"},{"key":"2026092513145772900_vgag238-B14","doi-asserted-by":"publisher","first-page":"993189","DOI":"10.3389\/fpls.2022.993189","article-title":"Improvement of heat stress tolerance in soybean (Glycine max L), by using conventional and molecular tools","volume":"13","author":"Jianing","year":"2022","journal-title":"Frontiers in Plant Science"},{"key":"2026092513145772900_vgag238-B15","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1007\/s11306-018-1465-2","article-title":"RANCM: A new ranking scheme for assigning confidence levels to metabolite assignments in NMR-based metabolomics studies","volume":"15","author":"Joesten","year":"2019","journal-title":"Metabolomics"},{"key":"2026092513145772900_vgag238-B16","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1007\/s12298-018-0597-1","article-title":"Effects of glyphosate on soybean metabolism in strains bred for glyphosate-resistance","volume":"25","author":"Li","year":"2019","journal-title":"Physiology and Molecular Biology of Plants"},{"key":"2026092513145772900_vgag238-B17","doi-asserted-by":"publisher","first-page":"1535","DOI":"10.1002\/mas.21731","article-title":"FT-ICR-MS-based metabolomics: A deep dive into plant metabolism","volume":"42","author":"Maia","year":"2023","journal-title":"Mass Spectrometry Reviews"},{"key":"2026092513145772900_vgag238-B18","doi-asserted-by":"publisher","first-page":"112193","DOI":"10.1016\/j.ecoenv.2021.112193","article-title":"Crop irrigation (soybean, bean, and corn) with enrofloxacin-contaminated water leads to yield reductions and antibiotic accumulation","volume":"216","author":"Marques","year":"2021","journal-title":"Ecotoxicology and Environmental Safety"},{"key":"2026092513145772900_vgag238-B19","doi-asserted-by":"publisher","first-page":"e064","DOI":"10.3989\/gya.0692141","article-title":"Fatty acid composition of soybean\/sunflower mix oil, fish oil and butterfat applying the AOCS Ce 1j-07 method with a modified temperature program","volume":"66","author":"Masson","year":"2015","journal-title":"Grasas y Aceites"},{"key":"2026092513145772900_vgag238-B20","volume-title":"MestReNova, version 8.1.2","author":"Mestrelab Research","year":"2013"},{"key":"2026092513145772900_vgag238-B21","volume-title":"Minitab statistical software, version 16.2.4","author":"Minitab Inc","year":"2013"},{"key":"2026092513145772900_vgag238-B22","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1021\/ac00101a004","article-title":"750 MHz 1H and 1H-13C NMR spectroscopy of human blood plasma","volume":"67","author":"Nicholson","year":"1995","journal-title":"Analytical Chemistry"},{"key":"2026092513145772900_vgag238-B23","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1007\/s44339-025-00018-w","article-title":"Addressing emerging contaminants in agriculture affecting plant\u2013soil interaction: A review on bio-based and nano-enhanced strategies for soil health and global food security (GFS)","volume":"2","author":"Nwankwo","year":"2025","journal-title":"Discover Toxicology"},{"key":"2026092513145772900_vgag238-B24","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.jplph.2011.08.009","article-title":"Impairment of carbon metabolism induced by the herbicide glyphosate","volume":"169","author":"Orcaray","year":"2012","journal-title":"Journal of Plant Physiology"},{"key":"2026092513145772900_vgag238-B25","doi-asserted-by":"publisher","first-page":"e231104","DOI":"10.2903\/j.efsa.2025.e231104","article-title":"Cumulative exposure of xenobiotics of emerging concern from agrifood under the One Health approach (XENOBAC4OH)","volume":"23","author":"Ortiz","year":"2025","journal-title":"EFSA Journal. European Food Safety Authority"},{"key":"2026092513145772900_vgag238-B26","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1016\/j.scitotenv.2017.04.214","article-title":"Fate of antibiotics in soil and their uptake by edible crops","volume":"599-600","author":"Pan","year":"2017","journal-title":"Science of the Total Environment"},{"key":"2026092513145772900_vgag238-B27","doi-asserted-by":"publisher","first-page":"6868","DOI":"10.3390\/su14116868","article-title":"Overview of environmental and health effects related to glyphosate usage","volume":"14","author":"Rivas-Garcia","year":"2022","journal-title":"Sustainability"},{"key":"2026092513145772900_vgag238-B28","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.chemosphere.2017.05.013","article-title":"A critical review on effects, tolerance mechanisms and management of cadmium in vegetables","volume":"182","author":"Rizwan","year":"2017","journal-title":"Chemosphere"},{"key":"2026092513145772900_vgag238-B29","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nature Methods"},{"key":"2026092513145772900_vgag238-B30","doi-asserted-by":"publisher","first-page":"2091","DOI":"10.1002\/etc.5700","article-title":"Uptake of pharmaceuticals by crops: A systematic review and meta-analysis","volume":"42","author":"Sleight","year":"2023","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2026092513145772900_vgag238-B31","doi-asserted-by":"publisher","first-page":"3538","DOI":"10.1002\/ldr.70327","article-title":"Microplastics and co-contaminants in soil: A review of combined ecological impact and emerging remediation strategies","volume":"37","author":"Tariq","year":"2026","journal-title":"Land Degradation & Development"},{"key":"2026092513145772900_vgag238-B32","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1093\/etojnl\/vgae075","article-title":"Spatial occurrence of emerging contaminants in rivers and wastewater. Analysis of environmental and human risks","volume":"44","author":"Teglia","year":"2025","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2026092513145772900_vgag238-B33","doi-asserted-by":"publisher","first-page":"14845","DOI":"10.1007\/s11356-024-32146-y","article-title":"Removal of enrofloxacin using Eichhornia crassipes in microcosm wetlands","volume":"31","author":"Teglia","year":"2024","journal-title":"Environmental Science and Pollution Research International"},{"key":"2026092513145772900_vgag238-B34","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.talanta.2017.02.030","article-title":"Simultaneous multi-residue determination of twenty one veterinary drugs in poultry litter by modeling three-way liquid chromatography with fluorescence and absorption detection data","volume":"167","author":"Teglia","year":"2017","journal-title":"Talanta"},{"key":"2026092513145772900_vgag238-B35","doi-asserted-by":"publisher","first-page":"179865","DOI":"10.1016\/j.scitotenv.2025.179865","article-title":"Assessing the phytotoxicity of emerging pollutants on vegetable crops grown with sewage effluent","volume":"989","author":"Tiwari","year":"2025","journal-title":"Science of the Total Environment"},{"key":"2026092513145772900_vgag238-B36","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1002\/etc.5582","article-title":"Generic model for plant uptake of ionizable pharmaceuticals and personal care products","volume":"42","author":"Trapp","year":"2023","journal-title":"Environmental Toxicology and Chemistry"},{"key":"2026092513145772900_vgag238-B37","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1590\/S0103-90162010000500007","article-title":"Alternative procedure for the cold test for soybean seeds","volume":"67","author":"Vieira","year":"2010","journal-title":"Scientia Agricola"},{"key":"2026092513145772900_vgag238-B38","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1111\/jipb.13631","article-title":"Breeding exceptionally fragrant soybeans for soy milk with strong aroma","volume":"66","author":"Xie","year":"2024","journal-title":"Journal of Integrative Plant Biology"},{"key":"2026092513145772900_vgag238-B39","doi-asserted-by":"publisher","first-page":"3966","DOI":"10.1016\/j.jia.2023.09.010","article-title":"Mapping and identification of QTLs for seed fatty acids in soybean (Glycine max L.)","volume":"23","author":"Zhong","year":"2024","journal-title":"Journal of Integrative Agriculture"}],"container-title":["Environmental Toxicology and Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/etc\/advance-article-pdf\/doi\/10.1093\/etojnl\/vgag238\/70896119\/vgag238.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/advance-article-pdf\/doi\/10.1093\/etojnl\/vgag238\/70896119\/vgag238.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:15:03Z","timestamp":1790356503000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/etc\/advance-article\/doi\/10.1093\/etojnl\/vgag238\/8778773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,1]]},"references-count":39,"URL":"https:\/\/doi.org\/10.1093\/etojnl\/vgag238","relation":{},"ISSN":["0730-7268","1552-8618"],"issn-type":[{"value":"0730-7268","type":"print"},{"value":"1552-8618","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,1]]},"article-number":"vgag238"}}