{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T12:27:05Z","timestamp":1793622425966,"version":"4.5.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Sichuan Provincial \u201c14th Five-Year Plan\u201d Crop and Livestock Breeding Research","award":["2021YFYZ0004"],"award-info":[{"award-number":["2021YFYZ0004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Against the backdrop of agricultural intensification and climate change, bee colonies are increasingly exposed to agrochemical residues and extreme temperatures, posing a growing threat to their survival. Glyphosate (GLY), one of the most widely used herbicides worldwide, and high temperature are two major environmental stressors associated with pollinator decline. However, their combined effects on queen larvae remain poorly understood. In this study, we examined the individual and combined effects of sublethal GLY exposure and 40\u2009\u00b0C heat stress on queen larvae of the honey bee Apis cerana cerana, with a focus on development, hormone levels, detoxification enzyme activities, and related gene expression. The results showed that GLY and high temperature significantly reduced the larval capping, emergence, and eclosion rates and decreased the body weight and abdominal weight of newly emerged queens. Under combined exposure, significant effects were shown for emergence weight, abdominal weight, juvenile hormone levels, glutathione S-transferase, and the expression of heat shock protein 83 and nuclear factor YC transcription factor\u2013related genes. In addition, co-exposure to GLY and high temperature significantly increased ecdysteroid levels and markedly altered the expression patterns of detoxification- and antioxidant-related genes. These results indicate that GLY and high temperature jointly disrupt endocrine regulation, detoxification metabolism, and key molecular responses in queen larvae, leading to more severe developmental impairment than either stressor alone.<\/jats:p>","DOI":"10.1093\/etojnl\/vgag248","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T03:57:07Z","timestamp":1789185427000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of glyphosate and high-temperature stress on queen larvae of the honey bee (\n                    <i>Apis cerana cerana<\/i>\n                    )"],"prefix":"10.1093","author":[{"given":"Bingrong","family":"Liu","sequence":"first","affiliation":[{"name":"Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University , Chengdu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songchuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University , Chengdu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University , Chengdu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiandong","family":"Yang","sequence":"additional","affiliation":[{"name":"Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University , Chengdu,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"2026110206465577300_vgag248-B1","doi-asserted-by":"publisher","first-page":"103784","DOI":"10.1016\/j.jtherbio.2024.103784","article-title":"The response of heat shock proteins in honey bees to abiotic and biotic stressors","volume":"119","author":"Abou-Shaara","year":"2024","journal-title":"Journal of Thermal Biology"},{"key":"2026110206465577300_vgag248-B2","doi-asserted-by":"publisher","first-page":"103194","DOI":"10.1016\/j.jtherbio.2022.103194","article-title":"Dynamics of heat shock proteins and heat shock factor expression during heat stress in daughter workers in pre-heat-treated (rapid heat hardening) Apis mellifera mother queens","volume":"104","author":"Al-Ghzawi","year":"2022","journal-title":"Journal of Thermal 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