{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T22:29:48Z","timestamp":1793485788808,"version":"4.5.0"},"reference-count":52,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:00:00Z","timestamp":1791590400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/V013041\/41"],"award-info":[{"award-number":["NE\/V013041\/41"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Current environmental regulations require the standardized testing of chronic ecotoxicological endpoints as part of their approval processes. However, although such tests tend to be resource intensive, new high throughput methods have not been favored to date due to their lack of standardization. To address this, a high throughput experiment that captured the conditions specified in the standardized Organisation for Economic Co-operation and Development (OECD)\u00a0Test Guideline 211 was carried out. Daphnids (&amp;gt; 400) were individually exposed to five concentrations of each of four polar narcotic chemicals (3,4-dichloroaniline, 2,4,5-trichloroaniline, 1,2-dichlorobenzene, 3-nitroaniline) and four chemicals that are nicotinic acetyl-choline receptor inhibitors (acetamiprid, clothianidin, nitenpyram, and thiacloprid) for a period of 21\u2009days. Growth and reproduction were measured every other day using a spheroid counter. Mortality in controls was low (&amp;lt; 20%) throughout the study period, meeting OECD validity criteria. However, average cumulative reproduction of 52 neonates per daphnid in controls was slightly below the OECD reproduction validity criteria of 60 neonates per daphnid. Despite variations in average observed size of the animals due to their position during measurement, results show a clear dose-response effect for growth and reproduction, enabling effect concentration to be calculated for all chemicals at any time point. The results of this experiment demonstrate a robust methodology that can generate a large volume of time sensitive data for chronic and acute endpoints under conditions consistent with standard OECD test guidelines in a cost and time-efficient manner.<\/jats:p>","DOI":"10.1093\/etojnl\/vgag266","type":"journal-article","created":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T19:54:27Z","timestamp":1791748467000},"source":"Crossref","is-referenced-by-count":0,"title":["High-throughput adaptation of standardized ecotoxicological tests for the model freshwater invertebrate\n                    <i>Daphnia magna<\/i>"],"prefix":"10.1093","author":[{"given":"Angel D","family":"Ceballos-Ramirez","sequence":"first","affiliation":[{"name":"Department of Environment and Geography, University of York , York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E","family":"Hodson","sequence":"additional","affiliation":[{"name":"Department of Environment and Geography, University of York , York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin D","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Environment and Geography, University of York , York,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas G","family":"Preuss","sequence":"additional","affiliation":[{"name":"Bayer AG , Monheim am Rhein,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elma","family":"Lahive","sequence":"additional","affiliation":[{"name":"UK Centre for Ecology and Hydrology , Wallingford,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7661-6242","authenticated-orcid":false,"given":"Daniel","family":"Burkow","sequence":"additional","affiliation":[{"name":"Bayer AG , Monheim am Rhein,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,10]]},"reference":[{"key":"2026103118013678900_vgag266-B1","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1016\/s0045-6535(01)00049-2","article-title":"Embryonic development assay with Daphnia magna: Application to toxicity of aniline derivatives","volume":"45","author":"Abe","year":"2001","journal-title":"Chemosphere"},{"key":"2026103118013678900_vgag266-B2","doi-asserted-by":"publisher","first-page":"2909","DOI":"10.1021\/es304784t","article-title":"Feeding inhibition explains effects of imidacloprid on the growth, maturation, reproduction, and survival of Daphnia magna","volume":"47","author":"Agatz","year":"2013","journal-title":"Environmental Science & Technology"},{"key":"2026103118013678900_vgag266-B3","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1039\/C7EM00328E","article-title":"Physiological modes of action across species and toxicants: The key to predictive ecotoxicology","volume":"20","author":"Ashauer","year":"2018","journal-title":"Environmental Science. 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