{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:42:19Z","timestamp":1772120539964,"version":"3.50.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To assess cardiac gene expression in peripheral blood cells of patients with idiopathic dilated cardiomyopathy (IDCM) and its relationship to echocardiographic left ventricular (LV) function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>A complete echocardiographic study and blood sampling were performed in 65 consecutive stable IDCM patients with LV ejection fraction (LVEF) 31.76 \u00b1 10.07% and chronic mild to moderate heart failure (NYHA functional class II to III) for \u22659 months. Blood samples from 19 healthy individuals were included for comparison. Transcript levels of myocardin, GATA4, alpha- and beta-myosin heavy chain (MHC), sarcoplasmic reticulum calcium ATPase 2 (SERCA2), and phospholamban were determined by quantitative real-time reverse transcription-polymerase chain reaction. Myocardin (24.88 \u00b1 4.93 vs. 3.98 \u00b1 1.12, P = 0.0048) and GATA4 (17.85 \u00b1 4.85 vs. 0.45 \u00b1 0.15, P = 0.0069 \u00d7 10\u22125) were upregulated in IDCM patients compared with controls, whereas SERCA2 (5.11 \u00b1 0.42 vs. 8.93 \u00b1 1.07, P = 0.001) was downregulated. In IDCM patients, myocardin (r = 0.279, P = 0.025), GATA4 (r = 0.314, P = 0.011), beta-MHC (r = 0.444, P=0.0002), and alpha-MHC (r = 0.272, P = 0.034) showed positive correlations, whereas SERCA2 (r = \u22120.264, P = 0.034) exhibited a negative correlation with LVEF. Patients with elevated LV filling pressures had lower myocardin (15.06 \u00b1 3.10 vs. 43.12 \u00b1 12.03, P = 0.048), GATA4 (8.96 \u00b1 2.17 vs. 34.38 \u00b1 12.60, P = 0.026), beta-MHC (10.59 \u00b1 4.05 vs. 16.43 \u00b1 4.91, P = 0.013), and alpha-MHC (0.27 \u00b1 0.08 vs. 0.79 \u00b1 0.20, P = 0.033) and higher SERCA2 (5.65 \u00b1 0.54 vs. 3.90 \u00b1 0.61, P = 0.037) levels. Patients with atrial fibrillation (AF) had higher SERCA2 levels compared with sinus rhythm patients (6.75 \u00b1 0.84 vs. 4.54 \u00b1 0.45, P = 0.017).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our data indicate that cardiac gene expression alterations in peripheral blood cells of IDCM patients may reflect alterations in LV function, whereas the presence of AF may be associated with increased SERCA2 levels in these patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/eurjhf\/hfq057","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T20:40:22Z","timestamp":1271191222000},"page":"541-548","source":"Crossref","is-referenced-by-count":21,"title":["Myocardial Gene Expression Alterations in Peripheral Blood Mononuclear Cells of Patients with Idiopathic Dilated Cardiomyopathy"],"prefix":"10.1093","volume":"12","author":[{"given":"Joanna E.","family":"Kontaraki","sequence":"first","affiliation":[{"name":"Molecular Cardiology Laboratory, School of Medicine, University of Crete , Crete ,","place":["Greece"]},{"name":"Department of Cardiology, University Hospital of Heraklion , PO Box 1352, Crete, 71110 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fragiskos I.","family":"Parthenakis","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital of Heraklion , PO Box 1352, Crete, 71110 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva G.","family":"Nyktari","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital of Heraklion , PO Box 1352, Crete, 71110 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandros P.","family":"Patrianakos","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital of Heraklion , PO Box 1352, Crete, 71110 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panos E.","family":"Vardas","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University Hospital of Heraklion , PO Box 1352, Crete, 71110 ,","place":["Greece"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,1,13]]},"reference":[{"key":"2026020203340850400_ejhfhfq057-bib-0001","doi-asserted-by":"crossref","first-page":"1807","DOI":"10.1161\/CIRCULATIONAHA.106.174287","article-title":"Contemporary Definitions and Classification of the Cardiomyopathies: an American Heart Association Scientific Statement From the Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and 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