{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T04:04:36Z","timestamp":1793073876974,"version":"4.5.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Data on ventricular pulsed-field ablation (PFA) are sparse in the setting of chronic myocardial infarction (MI). The objective of this study was to compare the biophysical and histopathologic characteristics of PFA in healthy and MI swine ventricular myocardium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Myocardial infarction swine (n = 8) underwent coronary balloon occlusion and survived for 30 days. We then performed endocardial unipolar, biphasic PFA of the MI border zone and a dense scar with electroanatomic mapping and using an irrigated contact force (CF)-sensing catheter with the CENTAURI System (Galaxy Medical). Lesion and biophysical characteristics were compared with three controls: MI swine undergoing thermal ablation, MI swine undergoing no ablation, and healthy swine undergoing similar PFA applications that included linear lesion sets. Tissues were systematically assessed by gross pathology utilizing 2,3,5-triphenyl-2H-tetrazolium chloride staining and histologically with haematoxylin and eosin and trichrome. Pulsed-field ablation in healthy myocardium generated well-demarcated ellipsoid lesions (7.2 \u00b1 2.1 mm depth) with contraction band necrosis and myocytolysis. Pulsed-field ablation in MI demonstrated slightly smaller lesions (depth 5.3 \u00b1 1.9 mm, P = 0.0002), and lesions infiltrated into the irregular scar border, resulting in contraction band necrosis and myocytolysis of surviving myocytes and extending to the epicardial border of the scar. Coagulative necrosis was present in 75% of thermal ablation controls but only in 16% of PFA lesions. Linear PFA resulted in contiguous linear lesions with no gaps in gross pathology. Neither CF nor local R-wave amplitude reduction correlated with lesion size.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Pulsed-field ablation of a heterogeneous chronic MI scar effectively ablates surviving myocytes within and beyond the scar, demonstrating promise for the clinical ablation of scar-mediated ventricular arrhythmias.<\/jats:p><\/jats:sec>","DOI":"10.1093\/europace\/euac252","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T06:52:05Z","timestamp":1676530325000},"page":"1503-1509","source":"Crossref","is-referenced-by-count":47,"title":["<i>In vivo<\/i>pulsed-field ablation in healthy vs. chronically infarcted ventricular myocardium: biophysical and histologic characterization"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4828-8480","authenticated-orcid":false,"given":"Uday","family":"Sandhu","sequence":"first","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laith","family":"Alkukhun","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1747-2859","authenticated-orcid":false,"given":"Babikir","family":"Kheiri","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8383-585X","authenticated-orcid":false,"given":"James","family":"Hodovan","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirby","family":"Chiang","sequence":"additional","affiliation":[{"name":"Galaxy Medical , 1531 Industrial Road, San Carlos, CA 94070 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Splanger","sequence":"additional","affiliation":[{"name":"VDx Veterinary Diagnostics and Preclinical Research Services , 215 C St #301, Davis, CA 95616 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7095-1181","authenticated-orcid":false,"given":"Quim","family":"Castellvi","sequence":"additional","affiliation":[{"name":"Galaxy Medical , 1531 Industrial Road, San Carlos, CA 94070 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4078-9064","authenticated-orcid":false,"given":"Babak","family":"Nazer","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute, Oregon Health and Science University , 3181 SW Sam Jackson Park Road, Portland, OR 97239 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"2023041606554605600_","doi-asserted-by":"crossref","first-page":"e010086","DOI":"10.1161\/CIRCEP.121.010086","article-title":"Primer on pulsed electrical field ablation: understanding the benefits and limitations","volume":"14","author":"Verma","year":"2021","journal-title":"Circ Arrhythm 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