{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T11:35:57Z","timestamp":1789472157173,"version":"build-2803163510"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Farapulse Inc."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Pulsed field ablation (PFA) is a novel, non-thermal modality that selectively ablates myocardium with ultra-short electrical impulses while sparing collateral tissues. In a proof-of-concept study, the safety and feasibility of ventricular PFA were assessed using a prototype steerable, endocardial catheter.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods and results<\/jats:title>\n                  <jats:p>Under general anaesthesia, the left and right ventricles of four healthy swine were ablated using the 12-Fr deflectable PFA catheter and a deflectable sheath guided by electroanatomic mapping. Using the study catheter, electrograms were recorded for each site and pre-ablation and post-ablation pacing thresholds (at 2.0\u2009ms pulse width) were recorded in two of four animals. After euthanasia at 35.5\u2009days, the hearts were submitted for histology. The PFA applications (n\u2009=\u200939) resulted in significant electrogram reduction without ventricular arrhythmias. In ablation sites where it was measured, the pacing thresholds increased by &amp;gt;16.8\u2009mA in the right ventricle (3 sites) and &amp;gt;16.1\u2009mA in the left ventricle (7 sites), with non-capture at maximum amplitude (20\u2009mA) observable in 8 of 10 sites. Gross measurements, available for 28 of 30 ablation sites, revealed average lesion dimensions to be 6.5\u2009\u00b1\u20091.7\u2009mm deep by 22.6\u2009\u00b1\u20094.1\u2009mm wide, with a maximum depth and width of 9.4\u2009mm and 28.6\u2009mm, respectively. In the PFA lesions, fibrous tissue homogeneously replaced myocytes with a narrow zone of surrounding myocytolysis and no overlying thrombus. When present, nerve fascicles and vasculature were preserved within surrounding fibrosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>We demonstrate that endocardial PFA can be focally delivered using this prototype catheter to create homogeneous, myocardium-specific lesions.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/europace\/euz341","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T12:43:45Z","timestamp":1575636225000},"page":"434-439","source":"Crossref","is-referenced-by-count":136,"title":["Endocardial ventricular pulsed field ablation: a proof-of-concept preclinical evaluation"],"prefix":"10.1093","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2221-8372","authenticated-orcid":false,"given":"Jacob S","family":"Koruth","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, PO Box 1030, New York, NY 10029, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kuroki","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, PO Box 1030, New York, NY 10029, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Iwasawa","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, PO Box 1030, New York, NY 10029, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raju","family":"Viswanathan","sequence":"first","affiliation":[{"name":"Farapulse Inc., Menlo Park, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Brose","sequence":"first","affiliation":[{"name":"Farapulse Inc., Menlo Park, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric D","family":"Buck","sequence":"first","affiliation":[{"name":"Farapulse Inc., Menlo Park, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elina","family":"Donskoy","sequence":"first","affiliation":[{"name":"Limited liability company\u2014Pathology Consultant"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srinivas R","family":"Dukkipati","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, PO Box 1030, New York, NY 10029, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek Y","family":"Reddy","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, One Gustave L. 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